内酰胺

The antibodies in plasma were also detected by indirect antiglobulin test. The result showed that levels of WBC,TB,DB,Glu and reticulocyte in six patients treated with β-lactam antibiotics were raised remarkably. DAT was strongly positive,but no RBC antibodies were detected in the plasma by IAT. The binding of red cells by monocytes or lymphocytes was great,and the hemolysis of red cells by alexin not appeared. After stopping above-mentioned β-lactam antibiotic administration,patient's WBC,TB,DB,and Glu levels returned to normal values.

结果发现：6例患者的临床治疗的药物都属于β内酰胺类；住院期间患者的WBC、TB、DB、Glu的检测结果出现异常，红细胞的DAT试验结果均为阳性，IAT试验结果均为阴性；将DAT阳性的红细胞进行补体结合实验时，结果为阴性；细胞涂片见部分红细胞表面有颗粒状物质附着，细胞培养见部分红细胞被白细胞识别、黏附；改用其它抗生素后，患者的上述化验指标恢复到正常值范围内，DAT试验结果转为阴性。

The result showed that levels of WBC,TB,DB,Glu and reticulocyte in six patients treated with β-lactam antibiotics were raised remarkably. DAT was strongly positive,but no RBC antibodies were detected in the plasma by IAT. The binding of red cells by monocytes or lymphocytes was great,and the hemolysis of red cells by alexin not appeared. After stopping above-mentioned β-lactam antibiotic administration,patient's WBC,TB,DB,and Glu levels returned to normal values.

结果发现：6例患者的临床治疗的药物都属于β内酰胺类；住院期间患者的WBC、TB、DB、Glu的检测结果出现异常，红细胞的DAT试验结果均为阳性，IAT试验结果均为阴性；将DAT阳性的红细胞进行补体结合实验时，结果为阴性；细胞涂片见部分红细胞表面有颗粒状物质附着，细胞培养见部分红细胞被白细胞识别、黏附；改用其它抗生素后，患者的上述化验指标恢复到正常值范围内，DAT试验结果转为阴性。

Modification of the side chain radical of β-lactam class antibiotic is a possible effective way,while the mother nuclide maintain the activity, but the different side chain radical alternating the drug tolerance of bacterium.

改造β-内酰胺类抗生素的侧链，不同侧链改变了细菌的耐药性，而其母核仍保持抗菌活性，这可能是克服抗生素使用剂量不断增大的一条有效途径。

The model has a good prediction at lower temperatures, while it is bad at higher temperatures where the solid phase was no existing and peptide product would be hydrolyzed because of the presence of amidase activity.

这是因为该模型未能考虑在偏高温度范围内酰胺酶活性对肽产物的水解作用，但这并不影响该模型对低温下肽合成产率变化的良好的预测性能。

Methods Retrospective analysis of the clinical data of 116 cases with hospital acquired respiratory tract infection by Haemophilus parainfluenzae during the period from Aug. 2002 to Jan. 2005 had been done. Used VITEK-60 microorganism autoanalysis system to appraise bacteria and its resistance against various antibiotics was carried out. Nirtocefin test was used to detect β-lactamase.

对2002年8月至2005年1月温州医学院附属第一医院住院的116例医院获得性副流感嗜血杆菌呼吸道感染的临床资料进行统计分析，对送检的痰或咽拭子标本用嗜血杆菌专用巧克力培养基进行分离培养，VITEK-60微生物自动分析系统进行鉴定以及药敏试验，用Nitrocefin纸片法测定被试菌的β-内酰胺酶。

This indicated that, with the different pH values in solution, the adsorption site of biliverdin could be changed.

在酸性溶液中，胆绿素分子环C上的氮原子和内酰胺环A上的氧原子发生了质子化。

Results bla and ADC genes were positive in the two strains of Acinetobacter baumannii.

结果 2株鲍曼不动杆菌中2种基因bla和β-内酰胺酶ADC基因呈阳性。

We selected mecA gene and femA factor asβ-lactam antibiotic-resistant genes, ermA, ermC, msrAgene as macrolides-lincosamids antibiotic-resistant genes, norA, grlA, gyrA, gene as Quinolonesantibiotic-resistant genes, and tetM gene as Tetracycline antibiotic-resistant genes on the basis of the latestresearch. We designed primers with GenBank enunciable gene sequence, cloned the drug resistantgenes, and sequenced these genes.

根据国内外最新文献报道，筛选出对β-内酰胺类抗生素耐药的mecA基因及辅助因子femA；对大环内酯类及林可霉素类抗生素耐药的ermA、ermC、msrA基因，对喹诺酮类抗菌药物耐药的norA、grlA和gyrA基因及对四环素类抗生素耐药的tetM基因作为拟选的主要耐药基因，分别设计PCR扩增的特异性引物，进行克隆。

Western blotting confirmed that the low activity of b-lactamase in E. coli Top10 pcs+ resulted from a lower amount of b-lactamase in its periplasm.

由此可见，周质内低含量的b-内酰胺酶是导致E.coli Top10 pcs+细菌氨苄青霉素抗性降低的原因。

Using the readily available optically active propargylic amines, the corresponding -α-chloroalkylidene-β-lactams with high ee values can be synthesized conveniently.

同时我们实现了由光学活性的炔丙基胺一步法生成光学活性的β-内酰胺化合物，这不仅在学术上而且在工业合成上都将具有非常重要的意义。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。