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DNA damage did not increase levels of the amiloride-sensitive sodium-hydrogen exchanger isoform 1 (NHE-1), intracellular pH, Bcl-x deamidation, and apoptosis.

DNA损伤并不增加细胞的阿米洛利敏感的钠-氢交换蛋白-1 (NHE-1)、细胞内pH值、Bcl -×脱酰胺作用及细胞凋亡水平。

Studing on samarium diiodide promoted asymmetric intramolecular reducing coupling reactions, we have made the resolution of the racemic biaryl dials by using enantiomeric N-tert-butanesulfinamine as the resolving reagent successfully and thus obtained a series of axially chiral biaryl dials with high enantiomeric excess.

中文摘要在二碘化钐促进的分子内不对称还原偶联反应中,我们不仅对联芳基二醛底物的合成路线进行了优化,还发现N-叔丁基亚磺酰胺辅基可以作为手性试剂,成功地对联芳基骨架二醛化合物进行拆分,得到高对映体过量的轴手性二醛类化合物。

To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.

为研究磷酸化应激诱导蛋白的表达、定位和功能,采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段,经产物纯化、与载体连接,分别构建了STIP1基因的原核表达载体和真核表达载体;IPTG诱导原核表达载体,变性聚丙酰胺凝胶电泳与染色后,可见相应大小的蛋白质表达;STIP1基因的真核表达载体转染细胞系后,共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内;用药物筛选STIP1基因转染的细胞后,流式细胞仪检测细胞周期,可见G〓-G〓期和G〓-M期的细胞明显降低,S期细胞明显增多。

Among the various electrophoresis technologies, the electrophoresis with a supporting media of polyacrylamide gel has become a common separate analysis method in lab and other fields, such as biochemistry research and clinical test, due to its steady chemical properties, excellent repeatability, small electroendosmosis, easy operation and high sharpness of separation.

在众多的电泳方法中,以高分子材料—聚丙烯酰胺凝胶为支持介质的电泳因其化学性质稳定、重复性好、电内渗小、操作简单及分辨率高等优点,已成为实验室常用的分离分析手段,在生化研究和临床检验等领域日益显示出其强大的生命力。

The treatment with abdominal cavity drainage,continuous gastrointestinal decompression,parenteral nutrition combined with enteral nutrition,glutamine,somatostatin and recombinant human growth hormone are the important factors for healing of anastomotic leakage following esophagogastrostomy or esophagojejunostomy after gastrectomy for gastric carcinoma.

保证腹腔引流的通畅、持续胃肠减压,特别是肠外结合肠内营养支持、强化谷氨酰胺、生长抑素、生长激素等措施,是促进胃癌切除术后食管胃或食管空肠吻合口漏愈合的重要手段。

To explore the safety, feasibility and the nutrition and immunonutrition effect of early postoperative enteral nutrition with glutamine in patients with gastroenteric tumor.

目的 探讨胃肠道肿瘤术后早期应用谷氨酰胺强化的肠内营养的可行性、安全性和对营养及免疫的影响。

Using early postoperative enteral nutrition with glutamine in patients with gastroenteric tumor is safe and effective. It is beneficial to the recovery of gastrointestinal function and effective after gastrointestinal operation and has a positive role in improving the patientg nutritional status.

胃肠肿瘤术后早期谷氨酰胺强化肠内营养是安全有效的,有利于胃肠功能的恢复减少住院时间和费用,对改善患者机体的营养及免疫状况具有积极意义。

In this paper,we used hexamethylene bisacetamide as a inducer to induce human mucoepidermoid carcinoma cell line MEC-1 cells to differentiate in vitro and vivo.The nucleolus organizer regions was investigated by means of the argyrophil technique.

本实验以六亚甲基二乙酰胺作为分化诱导剂,对涎腺粘液表皮样癌MEC-1细胞进行了体内外诱导,并通过银染技术对对照组和HMBA诱导组癌细胞核内核仁组成区的数目、大小及分布特征进行了观察。

Methods: The rats were injected with thioacetamide 600mg/kg twice at same time for two days to induce the model of acute hepatic failure rat except the normal group injected with the normal saline hypodermically.

按600mg/kg的剂量经皮下注射硫代乙酰胺1次,24小时后按相同剂量重复注射1次,造成大鼠急性肝衰竭模型,于实验前3天至实验结束分别给予各组大鼠生理盐水,不同剂量丹黄方及促肝细胞生长素,观察造模后48小时内大鼠病死率,并于造模48小时后经腹主动脉采血测定ALT、AST、TBil、TNF-α、IL-6,取肝组织作病检。

Methods The amides were prepared from tryptophan and different acid t1erivaties by the catalytic dehydration of dicyclohexyl carbodiimide. The characteristic heterocyclic ring system of 5-(3'-indolyl)-oxazoles was constructed by oxidative cyclization of amide, using dicholorodicyanoquinone.

以色氨酸为原料,与取代的苯甲酸在DCC催化下脱水缩合得到酰胺,再通过DDQ芐位氧化和分子内环合件成5-(3'-吲哚基)-恶唑。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。