内酰胺

Thus, patients who are intolerant of penicillin may also be intolerant of cephalosporins, and further, cephalosporins may induce anaphylaxis in patients with a history of penicillin anaphylaxis.

内酰胺类产品是一类具有β内酰胺环化学结构的产品，具有潜在过敏性可导致人过敏，由于对β内酰胺类的不耐受性，超敏性在此类产品性也可能存在而引起过敏，有例子表明在青霉素和头孢类之间存在交叉污染，因此，不能耐受青霉素的病人也可有耐受头孢产品，而且，头孢也可诱发有青霉素过敏史的人过敏。

Understand the therapeutic applications of β-lactam antibiotics and β-lactamase inhibitors.

了解β-内酰胺类、β-内酰胺酶抑制剂的临床应用。

We also observed the interaction between outer membrane permeability rate,β-lactamase and clinical effects of 5β-lactam antibiotics.

而且，进一步观察外膜通透率及β-内酰胺酶与5种β-内酰胺类抗生素临床疗效的关系。

The Staudinger reaction is one of the most important methods in synthesis of, 8-lactams. 3-(Thiophen-2-yl)-β-lactam is one class of important β-lactam derivatives.

Staudinger反应是合成β-内酰胺类化合物最重要的方法之一。3-（噻吩－2-基）井内酰胺是一类重要的β-内酰胺类衍生物。

Ketoheptamethylenimine was prepared by one-pot reaction of cycloheptanone with concentrated sulfuric acid. In this step, oximation and rearrangement occurred .

制备庚内酰胺时，我们采用了&一锅反应&的方法，环庚酮、浓硫酸和硫酸羟胺混合在一起，经过肟化、重排一次得到庚内酰胺。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

The pathogenic bacterium of prepartum and intrapartum infectious pneumonia mostly were G- bacilli,the pathogenic bacterium of postpartum infectious pneumonia mostly were G- bacilli and staphylococcus aureus.6.The drug resistant rates of G- bacteria to penicillins,cephalosporins,and monocyclicβ-lactam antibiotic were high,However,their compound of addingβ-lactamase inhibitor agents was relatively low resistance,and all G- bacteria were sensitive to carbapenem antibiotics.

产前及产时感染性肺炎的病原菌以G-杆菌多见，而产后感染性肺炎的病原菌主要为G-杆菌和金葡菌。6.G-菌对青霉素类、头孢菌素类及单环类的β内酰胺类抗生素耐药率高，但对其添加β内酰胺酶抑制剂的复合制剂耐药率较低，对碳青霉烯类抗生素均敏感；金葡菌对苯唑西林耐药率低，对万古霉素均敏感。7。

Methods Ampicillin inhibitionof E. coli Top10 pcs+ was tested at first, and then b-lactamase activity in periplasm was examined.

然后再使用抗生素抗性分析、b-内酰胺酶的酶活测定以及Western blot杂交技术，分析质粒编码的b-内酰胺酶从细胞质到细胞间质的分泌情况。

85 And 119486/79. They are a novel type of antibiotic having both the wide antibacterial spectrum and high safety of penicillin and cephem antibiotics belonging to beta -lactam antibiotics, as well as the potent antibacterial activity and high beta -lactamase stability of carbapenem antibiotics.Sodium-(5R, 6S)-6-[-1-hydroxyethyl]-7-oxo-3- [-2-tetrahydrofuryl]-4-thia-1-azabicyclo [3.2.0] hept-2-ene-2-carboxylate 5/2 hydrate (faropenem sodium, hereinafter referred to as compound 1) is currently used as an oral drug for various infectious diseases and is reported to show potent antibacterial activity against not only methicillin-sensitive Staphylococcus aureus, Streptococcus pyrogenes and Streptococcus pneumoniae but also gram-positive bacteria for which conventional beta -lactam drugs have proved ineffective such as penicillin-resistant pneumococci, oral staphylococci and enterococci, also showing a wide antibacterial spectrum covering gram-negative bacteria such as Haemophilus influenzae and anaerobic bacteria such as the genus Bacteroides, which activity is due to its novel skeleton penem ring (Kagaku Ryoho no Ryoiki The Field of Chemotherapy, Vol.

他们是一种新型的抗生素都具有广泛的抗菌谱和高度的安全青霉素和cephem抗生素，属于β-内酰胺类抗生素，以及作为强大的抗菌活性和高β-内酰胺酶稳定的碳青霉烯类antibiotics.sodium -（ 5 R ， 6 S ）-6 -[ - 1 -羟乙基] - 7 -氧- 3 - [ - 2 - t etrahydrofuryl] - 4 -硫杂- 1 -氮杂双环[ 3 。2.0]庚- 2 -节能- 2 -羧酸5 / 2水合物（法罗培南钠，以下简称为化合物1 ）是目前用来作为口服药物，为各种传染病和报道，以显示强大的抗菌活性，不仅对甲氧敏感的金黄色葡萄球菌，链球菌pyrogenes和肺炎链球菌，但也克阳性菌为常规β-内酰胺类药物已证明无效，如青霉素耐药pneumococci ，口腔葡萄球菌和肠球菌，也显示出广泛的抗菌谱，包括革兰阴性菌如流感嗜血杆菌和厌氧菌如属杆菌，这活动是由于其新颖的骨架青霉烯环（（化学ryoho没有ryoiki领域的化疗），第13卷，第10号，第74-80 ， 1997年）。

The therapeutic problems posed by class D beta-lactamases, a family of serine enzymes that hydrolyse beta-lactam antibiotics following an acylation-deacylation mechanism, are increased by the very low level of sensitivity of these enzymes to beta-lactamase inhibitors.

丝氨酸酶一个科能水解β-内酰胺抗生素和随后进行酰化和脱酰化机理， D类β-内酰胺酶引起的治疗问题，随着这些对β-内酰胺酶抑制剂的酶的非常低敏感性而不断增加。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。