内转肌

Results All cases became amenorrheic during GnRH-α therapy. The enlarged uteri all decreased to normal or near normal size. Menstruation returned in 80～90 days after cessation of treatment. Three cases conceived within four menstrual periods. One of them resulted in the birth of a healthy 3 150 g male at 38 weeks gestation by cesarean section. The second pregnancy resulting after adenomyomectomy was terminated by emergent cesarean section at 30 weeks gestation because of threatened rupture of uterus. The third is now normal at 28 weeks pregnancy .The fourth has had 2 menstrual periods and is still being followed up.

结果 GnRH-α用药期间4例均闭经，血雌二醇于用药的第3周降到绝经期水平；用药6次后增大的子宫缩小至正常或接近正常。1例子宫后壁局限性腺肌瘤未行手术切除者，用药后腺肌瘤缩小73.7%。3例于腹腔镜术后、停药转经的4个月内妊娠；另1例停药转经2个月，现在随访中。3例妊娠转归：1例足月剖宫产分娩；1例手术切除肌壁间腺肌瘤者，孕30周时因先兆子宫破裂急诊行剖宫产术；1例目前孕22周。

Their fecundity outcome were followed-up after cessation of GnRH-α treatment. Results All cases became amenorrheic during GnRH-α therapy. The enlarged uteri all decreased to normal or near normal size. Menstruation returned in 80～90 days after cessation of treatment. Three cases conceived within four menstrual periods. One of them resulted in the birth of a healthy 3 150 g male at 38 weeks gestation by cesarean section. The second pregnancy resulting after adenomyomectomy was terminated by emergent cesarean section at 30 weeks gestation because of threatened rupture of uterus.

结果 GnRH-α用药期间4例均闭经，血雌二醇于用药的第3周降到绝经期水平；用药6次后增大的子宫缩小至正常或接近正常。1例子宫后壁局限性腺肌瘤未行手术切除者，用药后腺肌瘤缩小73.7%。3例于腹腔镜术后、停药转经的4个月内妊娠；另1例停药转经2个月，现在随访中。3例妊娠转归：1例足月剖宫产分娩；1例手术切除肌壁间腺肌瘤者，孕30周时因先兆子宫破裂急诊行剖宫产术；1例目前孕22周。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

The purpose in the second part of the paper is investigating hANF gene expressions in rabbit myocardial tissue by intramyocardial injection of naked pCR3. hANF in vivo.

本课题第二部分在前期研究的基础上，将pCR3.hANF进行在体转染研究，通过心室肌内直接注射裸pCR3.hANF，观察兔心肌中hANF基因的表达。

Finally, we also examined the diferentiation efect of Ad-GFP on myoblasts.3.Study of the transfection of Ad-GFP to myoblasts which Transplanted in the intrarectal of the immune defect rat.The immune defect rats Were randomized in to 2 groups( 10 in each group):The experimental group transplanted with myoblasts transfected by Ad-GFP; the control group injected PBS.

并观察转染了Ad-GFP后是否会对成肌细胞的分化融合有影响。3、转染Ad-GFP的成肌细胞在免疫缺陷小鼠直肠内移植的实验研究将20只免疫缺陷小鼠随机分成2组(每组10只)，移植组肛管直肠粘膜下层注射转染Ad-GFP的成人成肌细胞，对照组注射PBS。

All internal fixators were removed and re-fixed with half ring sculcus external fixator. For 32 cases with soft tissue defect, 10 were repaired with gastrocnemius musculocutaneous flap, 6 with medial head of gastrocnemius and 16 with fascial flap.

所有病例均取出失效内固定后采用半环槽式外固定治疗。32 例软组织缺损和骨外露，10例经腓肠肌皮瓣转位覆盖，6例用腓肠肌内侧头转位加植皮消除创面，16例采用筋膜皮瓣修复。

Samples were harvested at 6 week and embedded in paraform, and ground sections of the bone tissue were prepared to observe green fluorescence under laser scanning confocal microscope. Propidium iodide staining of the sections was also performed for observation.

将其植入恒河猴背阔肌的肌袋内，以未转染GFP的BMSC用同样的方法接种块状可吸收HA上，作为对照，术后6周取材，4%的中性多聚甲醛固定，塑料包埋，制作骨磨片PI染色在激光共聚焦显微镜下进行观测。

The patients with DRS are treated with appropriate horizontal muscles recession in order to relieve abnormal head position and a significant tropia in primary position, prevent upshoot and downshoot on adduction in patients with DRS.

水平肌大量后徙可以使原在位的斜视得到矫正，代偿头位消失或得到明显改善，患眼内转的时候，眼球后退、睑裂变小明显减轻，上射和下射的现象减轻或消失。

The mechanical contraction of circle muscularis strips, the contractile response of dispersed smooth muscle cells, the change of 〓 stimulated by sennoside with different concentration and that stimulated by Ach after incubated with sennoside were evaluated. The translocation of RhoA by sennoside were measured by laser confocal scanting microscopy too.

检测不同浓度的番泻甙对小肠环行肌条和游离环行平滑肌细胞的收缩作用及对〓的影响，然后测定番泻甙对Ach刺激的小肠环行肌条、游离环行平滑肌细胞收缩反应和细胞内〓变化的影响作用，并通过免疫荧光实验检测番泻甙对RhoA的活化和空间转位的影响。

The procedure performed included 23 crossover saphenous vein bypasses 5 in situ saphenopopliteal bypasses 4 interventional therapies of iliac venous occlusion 2 muscular flap constructions on the popliteal vein 38 varicose vein strippings partly with the ligature of communicating venous and ulceration skingraftings 22 endovenous laser treatments of varicosis partly with ulceration skingraftings 17 simple ulceration skingraftings and 3 free flaps transfer for ulceration.

全组105例患者，共114条患肢，其中23条行大隐静脉交叉转流术， 5条行原位大隐静脉-腘静脉转流术， 4条行髂静脉介入治疗， 2条行腘静脉外肌袢形成术， 38条行曲张浅静脉剥脱术，部分联合交通静脉结扎和溃疡清创植皮术， 22条行曲张浅静脉腔内激光术，部分联合溃疡清创植皮术， 17条行单纯溃疡清创植皮术， 3条行溃疡清创联合游离皮瓣移植术。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。