内转

To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性，我们利用原位杂交和免疫组化等方法，研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动，结果表明：（1）热对各阶段的雄性生殖细胞都有损害，粗线期的初级精母细胞尤为敏感，实验性隐睾内的精原细胞尚未完全受到"致命"影响，说明精原细胞对热有一定的耐受性，但即使成为粗线期／双线期初级精母细胞，却未能获得由有丝分裂过渡到减数分裂的能力，呈现不同程度的凋亡，所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞，更谈不上长形精子细胞和精子的形成；（2）腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录，说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的；（3）正常兔睾丸组织中，〓在精原细胞和粗线期／双线期精母细胞中均有表达：cyclin B1蛋白在减数分裂前期的粗线期／双线期初级精母细胞中的表达量增加，于变态末期的精子细胞中达高峰。

The results showed that 1 transport of Gly-Pro was slightly quicker when Na+ replaced by K+ during the first period of 20 min;2 Na+ concentration gradient stimulated 4.6-fold of L-alanine transport, but had no effect on transport of Gly-Pro. Harmaline reduced transoport of L-alanine significantly but had no effect on Gly-Pro transport.

结果表明：(1)用K+替代Na+，并没有降低Gly-Pro在BBMV中的转运量，在孵育前20 min时间内，Gly-Pro的转运量稍稍提高；(2)Na+可使BBMV对L-丙氨酸的转运量提高到4.6倍，但不影响Gly-Pro的转运；二氢骆驼蓬碱显著地降低L-丙氨酸的转运，但对Gly-Pro的转运无影响。

By retrovirus vector the foreign mdr1 gene could be efficiently transferred into bone marrow mononuclear cells of mouse in vitro, and it was confirmed that mdr1 gene was expressing stably and effectively in cells; in short terms there is no significant influence on the bone marrow MNC by transfection of mdr1 gene,but in the long run(in our study we had surveyed 2 months),there is a decline in the expression of apoptosis factor Bax in the MNC which transfected by mdr1 gene,in favour of the recovery of the receptor mouse;on the other hand it also poses a blance problem between multiplication and apoptosis in the process of receptor hematopoiesis after transplanting HSC which was transfected with mdr1 gene.

通过逆转录病毒载体可在体外将外源性mdr1基因转入小鼠骨髓单个核细胞中，转染的mdr1基因能整合到骨髓单个核细胞基因组中并有功能性表达；mdr1基因转入小鼠骨髓单个核细胞在移植后短期内对受体造血细胞没有显著影响，但从长期看来(本研究观察2个月)转染了mdr1基因的造血干细胞的凋亡因子Bax表达降低，更有利于受体鼠造血的恢复；另一个方面它也同时提出一个问题，即mdr1基因转染造血干细胞在回输受体后造血过程的增殖凋亡平衡。

Thirdly, theoretical stress analysis of the drum is made, and a finite element stress and model analysis of LW500×1200 basket is made too. The result shows that the drum intension satisfied the demand, and sympathetic vibration will not happen in normal rotate speed. Comparing the real situation, the decanter centrifuge of this type meets the safe demand.

再次，介绍了高速离心机转鼓的理论强度计算公式，并应用有限元分析软件ANSYS对LW500 1200离心机转鼓做了有限元强度分析和振动模态分析，结果表明离心机转鼓满足强度要求，而且在正常转速范围内不会发生共振现象，对照该型号离心机实际现场应用的情况，本文所研究的钻井液离心机确实能够满足工作安全要求。

Third, in the aspect of influence of knowledge transaction cost factors and knowledge transfer mechanisms on knowledge transfer performances, knowledge transaction cost factors such as "trust","task uncertainty","information completeness","information asymmetry", and "knowledge tacitness" had direct and indirect influence on knowledge transfer performances.

在知识交易成本因子与知识移转机制其对知识移转绩效之影响方面，「信任」、「任务不确定性」、「资讯完备性」、「资讯不对称性」与「知识内隐性」等知识交易成本因子，则对知识移转绩效同时存在有直接与间接影响。

Results All cases became amenorrheic during GnRH-α therapy. The enlarged uteri all decreased to normal or near normal size. Menstruation returned in 80～90 days after cessation of treatment. Three cases conceived within four menstrual periods. One of them resulted in the birth of a healthy 3 150 g male at 38 weeks gestation by cesarean section. The second pregnancy resulting after adenomyomectomy was terminated by emergent cesarean section at 30 weeks gestation because of threatened rupture of uterus. The third is now normal at 28 weeks pregnancy .The fourth has had 2 menstrual periods and is still being followed up.

结果 GnRH-α用药期间4例均闭经，血雌二醇于用药的第3周降到绝经期水平；用药6次后增大的子宫缩小至正常或接近正常。1例子宫后壁局限性腺肌瘤未行手术切除者，用药后腺肌瘤缩小73.7%。3例于腹腔镜术后、停药转经的4个月内妊娠；另1例停药转经2个月，现在随访中。3例妊娠转归：1例足月剖宫产分娩；1例手术切除肌壁间腺肌瘤者，孕30周时因先兆子宫破裂急诊行剖宫产术；1例目前孕22周。

Their fecundity outcome were followed-up after cessation of GnRH-α treatment. Results All cases became amenorrheic during GnRH-α therapy. The enlarged uteri all decreased to normal or near normal size. Menstruation returned in 80～90 days after cessation of treatment. Three cases conceived within four menstrual periods. One of them resulted in the birth of a healthy 3 150 g male at 38 weeks gestation by cesarean section. The second pregnancy resulting after adenomyomectomy was terminated by emergent cesarean section at 30 weeks gestation because of threatened rupture of uterus.

结果 GnRH-α用药期间4例均闭经，血雌二醇于用药的第3周降到绝经期水平；用药6次后增大的子宫缩小至正常或接近正常。1例子宫后壁局限性腺肌瘤未行手术切除者，用药后腺肌瘤缩小73.7%。3例于腹腔镜术后、停药转经的4个月内妊娠；另1例停药转经2个月，现在随访中。3例妊娠转归：1例足月剖宫产分娩；1例手术切除肌壁间腺肌瘤者，孕30周时因先兆子宫破裂急诊行剖宫产术；1例目前孕22周。

Elastic fibers were well-distributed,and vascular endothelial cell did not immigrate.We suggested that expression of ectogenic VEGF triggered paracrine and autocrine of VEGF of chondrocyte and co-acted with VEGF receptor 2 to enhance permeability of chondrocyte and improve internal construct of engineering cartilage,and prevent vascularize proceed.

转VEGF基因软骨细胞作为组织工程的种子细胞与pluronic F-127复合后可于裸鼠体内形成转基因组织工程软骨，与对照组相比，转VEGF基因组织工程软骨具良好的生物学特性，结构均一且与正常软骨组织相似，软骨ECM的GAG、COLⅡ、COLⅩ增多，RunX2、Sox9表达增高，细胞处于增生期的肥大状态，初步分析其原因可能是转染后外源性的VEGF持续表达触发了软骨细胞VEGF自分泌，并通过VEGFR-2作用于软骨细胞，提高了软骨细胞活性，促进其存活与增殖，但未在软骨组织内引起血管内皮细胞的迁移及小血管形成。

Finally, we also examined the diferentiation efect of Ad-GFP on myoblasts.3.Study of the transfection of Ad-GFP to myoblasts which Transplanted in the intrarectal of the immune defect rat.The immune defect rats Were randomized in to 2 groups( 10 in each group):The experimental group transplanted with myoblasts transfected by Ad-GFP; the control group injected PBS.

并观察转染了Ad-GFP后是否会对成肌细胞的分化融合有影响。3、转染Ad-GFP的成肌细胞在免疫缺陷小鼠直肠内移植的实验研究将20只免疫缺陷小鼠随机分成2组(每组10只)，移植组肛管直肠粘膜下层注射转染Ad-GFP的成人成肌细胞，对照组注射PBS。

It is the first time to give the formulae of non diagonal matrix elements of tensor operator s which can be employed to describe the ro vibrational interaction for linear tetratomic molecules.

利用Lie代数方法研究了四原子分子振转相互作用，在代数框架内首次给出四原子分子振转相互作用的张量算子非对角矩阵元的表达式，利用这些表达式对线型四原子分子HCCF振转相互作用的l doubling进行了计

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。