内转

The results of these early research works showed that the localization of RNA polymerase I transcription was in the nucleoli and that of RNA polymerase II and Ⅲ in the nucleoplasm.

研究结果显示：RNA聚合酶Ⅰ的转录发生在核仁内，RNA聚合酶Ⅱ和RNA聚合酶Ⅲ的转录发生在核质中。

Lungs of conclusion children heart turns stream operation of orthoptic congenital heart disease can bring about the hurried inflammation He Kangyan such as TNF-α, IL-6, IL-8, IL-10, CRP inside the heart of disease medium release, turn in heart lungs the likelihood in shedding the systemic inflammation that cause to react produces main effect.

结论小儿心肺转流心内直视先天性心脏病手术可导致TNF-α、IL-6、IL-8、IL-10、CRP等促炎症和抗炎症介质的释放，在心肺转流诱发的全身炎症反应中可能发挥重要功能。

Hspa5 belongs to a member of the Hsp70 family. It plays important roles in facilitating fold and assembly of newly synthesized proteins and their translocation across the endoplasmic reticulum membrane as a molecular chaperone, helping refold stress-denatured soluble proteins and regulating signal transduction pathways by modulating the stability and activity of protein kinases and transcriptional factors.

Hspa5为Hsp70家族的成员，它可以作为分子伴侣促进新合成蛋白质的折叠、组装及其跨过内质网膜的转运；使应激变性的可溶性蛋白质重新折叠；通过调节蛋白激酶或转录因子的活性而控制信号转导途径。

On signal transduction of self-incompatibility in Brassica, the signal molecule-SCR ligand is produced by the pollen grain, recognized by SRK receptor in stigmatic papillae, and transduced into a cellular response.

在芸苔属植物的自交不亲和细胞信号转导过程中，信号分子-SCR配体是由花粉粒产生的，被柱头乳突细胞SRK受体识别后，进行细胞内信号转导。

The results shown that:(1) Bufo raddei Strauch tadpole was capable of lens regeneration, which originated from the epithelial cells at dorsal iris margin by depigmentation.(2) Depigmentation of Bufo raddei Strauch during lens regeneration was by two methods: First, pigment granules initially dispersed all over the cytoplasm were moved towards the periphery of the cell, and then were directly taken up by the amoeboid cells. Second, pigment granules in a cell were first crowded in a mass, surrounded by a membranous structure in the cytoplasm, which were eventually discharged as a mass from the cell.

结果表明：花背蟾蜍蝌蚪(1)具有晶状体再生的能力，其晶状体的再生来源于虹膜背缘的上皮细胞；(2)虹膜背缘去色素是转分化的前提，主要有两种方式：一种也是最主要的方式是在晶状体被摘除后虹膜外侧产生巨噬细胞，巨噬细胞吞噬虹膜色素上皮细胞中的色素颗粒；另一种方式是虹膜色素上皮细胞自身释放色素颗粒；(3)电镜观察发现虹膜色素上皮细胞转分化过程中线粒体有明显变化，核糖体增多，粗面内质网增多，晶状体纤维分化过程中细胞核逐渐凝聚。

The venous catheter has an effective anticoagulative function, and is easy to manipulate.

自制腹腔内静脉转流管抗凝性能优良，操作简便，转流过程中动物容易管理。

METHODS AND RESULTS: Using both primary peritoneal macrophages and studies in advanced atheromata in vivo, we introduce signal transducer and activator of transcription-1 (STAT1) as a critical and necessary component of endoplasmic reticulum stress/type A scavenger receptor-induced macrophage apoptosis.

方法和结果：通过使用原代腹膜巨噬细胞研究在体脂质斑块，我们引入信号转导子和转录激活子-1(STAT1)，作为内质网应激/A型清道夫受体诱导的巨噬细胞凋亡中关键和必要组分。

On strength computation,the finite element models of rotating mirror were established according to the real structure of the mirror,and the rotating three-faced beryllium mirror were analysed.Results display that the surface deformation magnitude of the mirror is approximately 0.334 6 μm,and the maximum stress is 0.204 GPa at 600 m/s of edge linear speeed.The regions of maximum stress are on the axial hole and symmetric to symmetrical planes of mirror body,and keep beelines to surfaces.On the modal analysis,the results show that the first and second modals of bending vibration exist around 3.4×105 r/min and 6.6×105 r/min,which appear binate and orthogonal respectively,and another modal belongs to the first torsional vibration that occurs by 5.13×105 r/min.

运用该方法得出转镜镜面变形量、内部应力分布和振动模态参量的定量数值：在边缘线速度600 m/s下，三面体铍转镜的最大变形量为约0.334 6 μm；最大应力0.204 GPa，位于轴孔附近并与镜面距离最短处；两个在互相垂直平面内的一阶弯曲振动模态发生在3.4×105 r/min附近，一阶扭振点位于5.13×105 r/min附近，两个二阶弯曲振动点位于6.6×105 r/min附近。

Methods Westernblot was employed to detect the intracellular expressions of caspase-9, hTERT, Bcl-2 and Bax, and mitochondrial and cytoplastic cytochrome C in HepG2 cells transfected by pSliencer 3.1-H1 neo-shTERT.

收集第20代hTERT基因RNAi真核表达载体论定转染细胞、对照细胞及未转染HepG2细饱，采用Western blot检测线粒体，胞质细胞色素C的表达及细胞内caspase-9、Bcl-2、Bax和hTERT的表达。

To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.

为研究磷酸化应激诱导蛋白的表达、定位和功能，采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段，经产物纯化、与载体连接，分别构建了STIP1基因的原核表达载体和真核表达载体；IPTG诱导原核表达载体，变性聚丙酰胺凝胶电泳与染色后，可见相应大小的蛋白质表达；STIP1基因的真核表达载体转染细胞系后，共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内；用药物筛选STIP1基因转染的细胞后，流式细胞仪检测细胞周期，可见G〓-G〓期和G〓-M期的细胞明显降低，S期细胞明显增多。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。