内转

Metonymic expressions of emotional conception result from replacing abstract category with concrete one within a cognitive domain. Therefore attention should be paid to specific context for better understanding of metonymy.

情感概念转喻是在某一认知框架内以具体代替抽象的结果，同时对转喻的理解离不开特定的语境。

We got the result: Bufo Raddei Strauch Tadpole (1) Bufo Raddei Strauch Tadpole was capable of lens regeneration, which originated from the epithelial cells at iris dorsal margin by dipigmentation.(2) Dipigmentation of Bufo Raddei Strauch by two methods: one was licked up by amoebocyte, the other one was flowed from the pigment cells.(3) In the lens regeneration cells of Bufo Raddei Strauch profiles of the mitochondria became complicated ribosome manifold, and the endoplasmic reticulum was cisternal dilation. During the process of deferentiation of lens fiber the cell nucleolus became little and chromatin shrinked.(4)The crystalline electrophoresis of Bufo Raddei Strauch Tadpole lens regeneration showed that during lens regeneration P-crystalline was expressed earliest then was γ-crystalline and α-crystallin, this result is consistent with newt.

结果表明：花背蟾蜍蝌蚪(1)具有晶状体再生的能力，其晶状体的再生来源于虹膜背缘的上皮细胞；(2)虹膜背缘去色素是转分化的前提，主要有两种方式：一种也是最主要的方式是在晶状体被摘除后虹膜外侧产生巨噬细胞，巨噬细胞吞噬虹膜色素上皮细胞中的色素颗粒；另一种方式是虹膜色素上皮细胞自身释放色素颗粒；(3)电镜观察发现虹膜色素上皮细胞转分化过程中线粒体有明显变化，核糖体增多，粗面内质网增多，晶状体纤维分化过程中细胞核逐渐凝聚；(4)SDS-PAGE电泳发现，花背蟾蜍蝌蚪晶状体再生中相关蛋白表达顺序与有尾类蝾螈相似，蛋白表达顺序依次是β-晶状体蛋白、γ-晶状体蛋白和α-晶状体蛋白，并且后两种蛋白几乎同时表达。

BACKGROUND: Preliminary study shows that the FHL1 gene expression is down-regulated in muscle tissue of patients with clubfoot, and the gel retardation experiments have verified HOXD13 and FHL1 gene promoter region transcription factor predicted binding sites in vitro, but the experimental results are not enough to truly reflect in vivo transcriptional regulatory protein and DNA binding conditions.

摘要背景：前期研究表明，在马蹄内翻足患者肌肉组织中存在FHL1基因表达下调，并利用凝胶阻滞实验在体外初步验证了HOXD13和FHL1基因启动子区转录因子预测位点的结合作用，但凝胶阻滞实验结果不一定能真实地反映体内转录调控蛋白和DNA结合的状况。

ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.

ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子，在许多同种的真核生物有它的同源基因，这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关，而这个家族的蛋白质和DNA的结合能，各种ARID蛋白质的专一性尽相同，过大致上偏好於和AT-rich的序结合；我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因，分别是garid1和garid2，我们首先对於garid1做分析；将AU1标记接到gARID1转染形鞭毛虫，用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下，过其阳性染色和蛋白质表现有明显增加；EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合，经由突变序分析，也显示gARID1的结合序为AGATC和AATAAAATA，随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。

The method comprises: utilizing arctiin as the precursor compound, and converting arctiin into 4, 4'-dihydroxy enterolactone by intestinal bacteria in an anaerobic condition. 4, 4'-dihydroxy enterolactone is lignin compound, and can be converted into phytoestrogen in human intestinal canals.

本发明是以牛蒡苷为先导化合物，在厌氧条件下，以肠道细菌将牛蒡苷转化为4，4′-二羟基肠内酯。4，4′-二羟基肠内酯为木脂素类化合物，在人的肠道中能被转化为植物雌激素―肠内酯而发挥药理作用。

Oncogenes, existing widely in eukaryotic cells, take part in the processes of cell prolification and differentiation. c-fos and c-jun are two important oncogenes in CNS, and can be rapidly expressed in response to stimulations and injuries of nervous system, Oncogene proteins play very important rules as "nuclear third messengers"during signal transduction in CNS.

癌基因广泛存在于真核细胞内，参与细胞的增殖分化等活动。c-fos和c-jun是神经系统内两种十分重要的癌基因，受到外来刺激或神经系统损伤时，可以有快速的表达，其编码的蛋白产物作为一种&核内第三信使&参与调节细胞的信号转导过程。

The remaining eighteen pigs, served as three viral administered groups, underwent coronary artery ligation and received intramyocardial viral injection. rAAV- GFP group (n=6), rAAV-CD151 group (n=6) and rAAV-antiCD151 group (n=6) animals respectively received direct intra- myocardial injection of rAAV-GFP, rAAV-CD151 and rAAV-antiCD151 1×10~(12 viron particles per pig, at 10 sites correspondingly.

采用三质粒共转染法包装CD151、antiCD151和GFP重组腺相关病毒(recombinant adeno-associated virus，rAAV)。2.1月龄小型猪22头，随机分为4组，分别为正常对照组4头(不予冠脉结扎和注射病毒，正常喂养)，rAAV-GFP组6头结扎冠状动脉左前降支，心肌内分10点注射1×10~(12viron particles的rAAV-GFP病毒，rAAV-CD151组6头结扎冠状动脉左前降支，心肌内分10点注射1×10~(12viron particles的rAAV-CD151病毒，rAAV-antiCD151组6头结扎冠状动脉左前降支，心肌内分10点注射1×10~(12vironparticles的rAAV-antiCD151病毒。

Test tasks: to check whether the loose connector; hand plate coupling the rotor to move a few laps to see whether rotation and flexible unit, whether there is uneven sound and light and heavy feeling, to determine whether pump or foreign body axis is curved seals, such as the installation is correct; inspection seal on the 20th cavity clean lubricants are added 1 / 2 cavity space; pump unit surface is clean; unit no-load test shift.

试车工作：检查连接件是否松动；用手盘动联轴器使转子转数圈，看机组转动是否灵活，是否有响声和轻重不匀的感觉，以判断泵内有否异物或轴是否弯曲，密封件安装正不正等；检查密封腔内的清洁20号润滑油是否加注1/2腔内空间；泵机组表面是否干净；机组转向空载测试。

Test job: to check whether the connections loose; hand plate coupling the rotor to move a few laps, watch crew rotation is flexible, it has the sound and feeling of the severity of Unevenness to determine whether there is foreign body pump or shaft is bending, sealing the installation is not correct, etc.; inspection seal cavity clean lubricants on the 20th whether or not add 1 / 2 cavity space; pump unit surface is clean; crew turned no-load test.

试车工作：检查连接件是否松动；用手盘动联轴器使转子转数圈，看机组转动是否灵活，是否有响声和轻重不匀的感觉，以判断泵内有否异物或轴是否弯曲，密封件安装正不正等；检查密封腔内的清洁20号润滑油是否加注1/2腔内空间；泵机组表面是否干净；机组转向空载测试。

"Working with mice, we found that glucose levels were elevated and there was glucose production from a 'precursor', a source not normally metabolised."Other results in the study show that to some extent ARNT is regulated by insulin, so that insulin resistance in itself will contribute to a decrease in ARNT. If liver cells are treated with insulin, there will be a small increase in ARNT protein. The insulin will also help move the ARNT into the nucleus of the cell, where it does its job as a master regulator.

胰岛素绝对或相对不足时，肝脏内一种称之为 ARNT 的转录因子减少达90%，而 ARNT 与控制肝脏生产和释放葡萄糖的基因表达有关， ARNT 减少，肝脏生产和释放葡萄糖增加。2型糖尿病时，胰岛素相对不足不仅使肝脏内 ARNT 减少，也导致胰岛β细胞内 ARNT 减少，使得胰岛素生成减少和释放延迟。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。