内质的

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

In the present study changes of NOS positive cells after injection of LPS and apoptotic ependymal cells, the relationship between expression of immediate early gene c-fos and astrocytes, and comparison of changes between SFO and rest regions of the brain in different time-points were investigated with NADPH-d histochemistry, apoptosis in SITU detection of TUNEL, TEM and SEM, fluorescein double labeling of immunohistochemistry, and laser scanning confocal microscope .

为了证实穹窿下器在脑内免疫调节系统中的作用，在本研究当中，应用了NADPH-d的组化方法、TUNEL末端标记法、扫描及透射电镜方法、免疫组织化学的荧光双标法及激光扫描共聚焦显微镜技术，来研究在腹腔内给予大肠杆菌内毒素后，脑内的一氧化氮合酶细胞的变化、室管膜细胞的凋亡、早期即刻基因c-fos的表达与星形胶质细胞之间的关系，以及穹窿下器与其它脑区相比较时的特异性改变。

In the present study changes of NOS positive cells after injection of LPS and apoptotic ependymal cells, the relationship between expression of immediate early gene c-fos and astrocytes, and comparison of changes between SFO and rest regions of the brain in different time-points were investigated with NADPH-d histochemistry, apoptosis in SITU detection of TUNEL, TEM and SEM, fluorescein double labeling of immunohistochemistry, and laser scanning confocal microscope.

为了证实穹窿下器在脑内免疫调节系统中的作用，在本研究当中，应用了NADPH刁的组化方法、TUNEL末端标记法、扫描及透射电镜方法、免疫组织化学的荧光双标法及激光扫描共聚焦显微镜技术，来研究在腹腔内给予大肠杆菌内毒素后，脑内的一氧化氮合酶细胞的变化、室管膜细胞的凋亡、早期即刻基因（immediate early genes沁－fos的表达与星形胶质细胞之间的关系，以及穹窿下器与其它脑区相比较时的特异性改变。

Substance P distributes nonuniformly in the striatum and globus pallidus.The postnatal development of this neurotransmitter has significant difference with other neurotransmitters in the brain.

P物质非均匀性地分布在纹状体和苍白球内，纹状体和苍白球内P物质的生后发育与其他神经递质有显著的差异。

Yantai Normal College,264025,ChinaABSTRACT Most epithelial nuclei of Nuttallia olivacea are elliptic in shape and the chromatin is filiform.Most nuclei of connective tissue are round and have Feulgen positive granules.

摘 要 紫彩血蛤的鳃上皮细胞核多为椭圆形，核内染色质呈丝状；结缔组织细胞的核多为圆形，部分核内有块状Feulgen阳性颗粒。

Western blotting confirmed that the low activity of b-lactamase in E. coli Top10 pcs+ resulted from a lower amount of b-lactamase in its periplasm.

由此可见，周质内低含量的b-内酰胺酶是导致E.coli Top10 pcs+细菌氨苄青霉素抗性降低的原因。

In order to clear heterogeneous reservoirs waterflooding performance, and decreased interlayer and within-layer contradictions, and improved the water flooding recovery, experimental study should be conducted first, This paper based on Pucheng Sasan reservoir sandstone cores to study object. Multi-point pressure measurement of water flooding oil experimental apparatus was established. Through the three-layer water flooding experiment to study injection strategy, interlayer interference and recovery factors. True sandstone micro-model water flooding used newly developed color visual image analysis system researched water-drive types, forms of residual oil and waterflood efficiency. Percolation mathematical model was established, black oil simulation program was developed, long-core displacement experimental results were matched, pressure and saturation change were researched by the simulation matching results.

为了明确非均质储层注水开发动态过程，减轻由于层间和层内非均质造成的开发矛盾，提高水驱采收率，本文从实验研究出发，以濮城沙三储层岩心为研究对象，建立了一套带多测压点的多层长岩心水驱油实验装置，通过开展三层水驱油实验，研究注水方式、层间干扰以及采收率影响因素；真实砂岩微观模型水驱油实验采用最新研制的彩色可视化图像分析系统研究水驱类型、残余油形成类型以及水驱油效率；建立并求解渗流数学模型，编制黑油模拟程序，对长岩心驱替实验进行拟合，通过拟合结果研究驱替过程中油水饱和度和压力的变化情况等研究工作。

One of them was identified as aldehyde dehydrogenase 2, and the others were both identified as mouse RIKEN cDNA 2900053E13, suggesting that the protein might be post translationally modified.

通过对差异蛋白的胶内酶解和高效液相色谱／离子阱质谱鉴定，发现其中一个蛋白点为乙醛脱氢酶2（aldehyde dehydrogenase 2），另外两个差异点鉴定结果为同一个蛋白RIKEN cDNA 2900053E13，提示该蛋白在正常和心肌缺血心肌的线粒体内修饰状态发生了变化。

Moreover, overpression of Wnt10b in the organ culture of vibrissae HF has been manipulated by infected the recombinant adenovirus expressed Wnt10b protein(Adwnt10b).

Ki67在毛母质中的表达变化规律以及在毛母质中的表达部位与Wnt10b具有一致性。2、我们对Wnt10b重组腺病毒进行了逐级扩增并应用氯化铯超高速密度梯度离心法纯化，病毒最终滴度达到109 PFU/ml；3、首次发现加入Wnt10b重组腺病毒可明显促进体外器官培养的触须毛囊毛干和内根鞘生长，这与本课题组在在体动物实验中发现的现象一致。

The percentage of active transmission of small brown plant hopper varied with the infection stage of RBSDV in maize cell while the nymph acquiring feeding. There were 2.93% and 7.83% of viruliferous rate resp

用灰飞虱无毒若虫在细胞内病毒基质出现和病毒增殖期饲毒的，到成虫时分别有2.93%和7.83%个体传毒率；在细胞内病毒成串分布于管状结构和晶格状聚集排列期饲毒的，到成虫时均不能传毒。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。