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Results The amaranthine nuclei had a distinct outline and distributed evenly on the slide. The area of nuclei in liquidbased preparation was much smaller and chromatin was more condensed in comparison with those in conventional smear. The nuclei in conventional smears had the largest value of area and the value of AOD, and CV of IOD, the IOD ratio among diploid, tetraploid and octaploid DNA was most close to 2 and 4, and the CV of IOD exceeded 6%.

结果 三种涂片内肝细胞核分布均匀,轮廓清晰,呈紫红色,与传统涂片法相比,液基法内肝细胞核面积明显减小,染色质高度浓缩;相同倍体的肝细胞核在传统涂片中面积最大,平均光密度最低,各倍体间的比值最接近2和4,积分光密度CV值最低(。5)。

In addition, spermiogenesis includes chromatin condensation, migration of the cytoplasm and mitochondria to the caudal pole of the nucleus, formation of a moderate axial nuclear fossa containing the proximal centriole and part of the distal centriole and presence of a short cytoplasmic canal separating mitochondria from the flagellum.

另外,精子发生过程中还包括染色质浓缩,细胞质和线粒体向细胞核的尾端迁移,在核的后端中轴位置上形成中等大小的核后凹,近端中心粒和远端中心粒的一部分嵌在核后凹之内,短的胞质内陷管将线粒体与鞭毛分隔开。

Histological observation was performed. Results The peak of serum CINC concentrations occurred 3 h after reperfusion with the difference between the groups with and without ONO-5046 being significant. Expression peak of CINC mRNA in the pancreatic graft occurred 3 h after reperfusion with the expression level in the ONO-5046 treated group significantly lower than in the ONO-5046 untreated group. The activity of MPO in the treated group was obviously decreased as compared with in the untreated group.

结果 CINC于再灌注后3小时出现峰值,治疗组为(7.24±1.46)μg/L,对照组为(28.4±1.85)μg/L,差异显著;移植物中CINC mRNA于再灌注后3小时表达最强,治疗组的表达水平明显低于对照组;MPO的活性,治疗组显著低于对照组;治疗组的胰小叶间质水肿及胰小叶间质和胰小叶内的中性粒细胞浸润较轻。

The Colburn heat transfer factors Jh correlation could describe 96.6% of experimental data and the Colburn mass transfer factors Jm correlation could describe 81.4% of experimental data with an error of ±15%.

开发了反映空气侧传热特性的Colburn传热因子Jh和反映传质特性的Colburn传质因子Jm的关联式,所开发关联式在±15%误差范围内分别能涵盖96.6%和81.4%的实验数据,其平均误差分别为5.3%和8.5%。

METHODS: The level of intracellular calcium of human microglia grown on coverslip,which was loaded by calcium-probe,Fluo-4,and then treated in various experimental processing,was detected by confocal microscopy with time resolution mode.The binding of gp120 to human microglia was determined with confocal microscopy or flow cytometry after treatment with gp120 and stained with anti-gp120-FITC antibody.Phosphorylation of ERK within human microglia with or without gp120 stimulation was analyzed with confocal microscopy following the direct immuno-staining with anti-phosphorylated ERK antibody.

用钙离子探针Fluo-4标记粘附在盖玻片上的人小胶质细胞,运用共聚焦显微镜以荧光强度为指标实时观察各种条件下的细胞内钙离子水平的变化;用gp120处理并用anti-gp120-FITC进行染色,运用共聚焦显微镜术和流式细胞术分析人小胶质细胞与gp120结合情况;用抗磷酸化ERK 抗体免疫荧光方法进行染色,运用共聚焦显微镜术和流式细胞术进行ERK磷酸化水平分析。

Methods The immunofluorescency, Western blotting and RT-PCR were performed on mouse liver tissue obtained from 4 experimens pcDNA3.1(+-HBX and 1 normal control, 24 h after delivery the HBX gene eukaryon expression vector pcDNA3.1-HBX into mouse by the means of hydrodynamic injection.

实验动物分成模型组和对照组,模型组利用已构建的含有HBX基因的真核表达质粒pcDNA3.1-HBX,对照组以等量生理盐水代替,采用流体动力学法将质粒经尾静脉高压注入小鼠体内,24h后取小鼠肝组织,行免疫荧光、RT-PCR和Western blot法从不同水平检测HBX在小鼠肝组织内的表达情况。

The flumes hall has a sediment characteristic analysis room and seven advanced glass flumes with automatic controlling and measuring systems, where researches can be conducted on flow structure in curved channel, movement of suspend load, movement of bed load, movement of hyper concentrated sediment flow, movement rules of sediment caused by wave and current flow together, or by the wind, channel pattern transformation and the settling characteristics of sediment etc.

水槽实验大厅拥有本学科内国内外自动化程度最高、测控技术最先进、规模最大的浑水变坡玻璃实验系列水槽共七座以及泥沙基本特性分析室,可以进行弯道水流结构、悬移质泥沙运动、推移质泥沙运动、高含沙水流运动、波流、径流共同作用下泥沙运动规律、河型转化、风作用下泥沙运动规律的研究、泥沙的沉降特性等。

Hyphal growth rate method was used to determine the inhibitory activities of chitooligosancharide against the pathogenic fungus of tobacco brown spot, Alternaria alternate Keissler, in the laboratory, and tobacco plants were applied with COS and its combinations like COS + Trichoderma + chitin with 5 treatments to control the disease under conditions of pot culture and inoculation in the greenhouse. Activities of 4 defensive enzymes, superoxide dismutase, polyphenol oxidase, peroxidase and chitinase, in tobacco plants were determined systematically at the same time within 10 days after application of COS and inoculation with the pathogenic fungus.

在实验室条件下,采用菌丝生长速率法测定了几丁寡糖对烟草赤星病菌的抑制活性,继而在温室盆栽和人工接种条件下,分别研究了几丁寡糖、几丁寡糖+木霉+几丁质等5种方法处理对烟草赤星病的防治效果,同时分别系统测定了烟草植株施用几丁寡糖及人工接种赤星病菌后10 d内SOD、POD、PPO和几丁质酶等4种防御酶的活性。

A series of thermodynamic parameters were obtained by third-order polynomial fitting Van't Hoff plots. The thermodynamic partitioning behavior of those solutes was investigated.

在288~323 K范围内测定了中性芳香族溶质在脂质体电动色谱中的分配系数,通过三项式拟合Van't Hoff图获得了一系列的热力学参数,研究了溶质在脂质体电动色谱中的热力学分配行为。

Partition coefficients of neutral aromatic solutes in LEKC between 288~323 K were measured. A series of thermodynamic parameters were obtained by third-order polynomial fitting Van't Hoff plots. The thermodynamic partitioning behavior of those solutes was investigated. The results showed that the partition coefficient became greater with the increment of temperature and the number of CH2 functional groups on benzenering.

在288~323K范围内测定了中性芳香族溶质在脂质体电动色谱中的分配系数,通过三项式拟合Van't Hoff图获得了一系列的热力学参数,研究了溶质在脂质体电动色谱中的热力学分配行为。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。