内质的

Methods Eukaryotic expression plasmid pCMVCD was constructed , and identified by re2 striction endoenzyme digestion. CD gene was transfected into NSCs from new2born Wistar rats using Lipofec2tamine2000. Positive clones (named NSCs/ CD cells) were screened by G418 presence. 52Fluorocytosine (52FC) ad2ministration of different concentrations were incubated with NSCs/ CD cells. NSCs/ CD cells viability ratios were mea2 sured by MTT assay.

通过构建真核表达质粒pCMVCD ，限制性内切酶消化鉴定后，采用Lipofectamine 2000 脂质体介导法转染新生大鼠室管膜下区神经干细胞(Neural stemcells ， NSCs)，G418 筛选阳性克隆，加入不同浓度的52氟胞嘧啶(52Flourocytosine ， 52FC)，MTT比色法测定NSCs的生存率。

Test results show that the bed material＇ s density is the main influencing factor. When sand is taken as the bed material, light and easily inflammable objects such as wood, paraffin, rubber etc can get evenly mixed in the bed, while heavy incombustible matter can adequately be segregated.

结果表明：密度是主要影响因素；以砂子为床料的内循环流化床，能够实现木块、石蜡、橡胶等轻质可烯物的均匀混合，同时灰渣等重质不可燃物能够充分分离。

During the secondary wall formation of fiber, H+-ATPase, Ca2+-ATPase and APase also sustainablely remained in plasma membrane, plasma membrane invagination, plasmodesmata, transfer vesicles and agglutinated nucleus.

在次生壁形成的整个过程中，ATP酶、Ca2+-ATPase和酸性磷酸酶在运输小泡、细胞膜、质膜内陷、胞间连丝和凝聚的染色质中将持续存在。

Keeping［element］/［Mg］concentration ratio lower than 0.05 can minimize isobaric interference and matrix effects.

控制［元素］/［Mg］的浓度比在0.05范围内，可以忽略同质异位素的干扰和基质效应。

When the value of m is on a neighbourhood of 2, the value of η can achieve maximum, on this condition, strength-size effect is very obvious.

在1.65≤m≤3范围内变化最为剧烈，所以当非均质参数m在此范围内时，岩石材料的强度尺寸效应在明显和不明显之间变化幅度最大。

The method can be used in all kinds of domains since it calculates the global minimum of a constrained multi-extrema function to get high-quality centroidal Voronoi tessellations for the region.

该算法结合k-means方法和模拟退火算法求解约束条件下的多峰值函数全局最小值，在待求计算域内和边界自动生成无网格计算节点，且节点为计算域对应质心Voronoi结构的质心点。

Astrocytes constitute nearly half of the cell in brain, but its funtion is a long-standing neurobiological mystery.

星形胶质细胞是脑内数量最多的一类胶质细胞，与神经系统生长发育，功能活动及疾病的形成有密切关系。

Brain injury in rat activated NF-κB for long time. During the first 1-2hours after injury, activated NF-κB existed mainly in axon, and then appeared inastrocyte in the next 24 hours, lasted for 1 year in pallium.

鼠脑损伤后可致NF-κB的长时间激活，创伤后1～2小时内，激活的NF-κB主要存在于轴突，在创伤24小时后激活的NF-κB主要存在于受伤皮质内的小神经细胞和星形胶质细胞内，这种激活可在脑皮质中至少持续1年。

Prussian blue staining showed numerous blue stained fine particles in the cytoplasm of 99% BMSCs.

普鲁士蓝染色结果显示99%以上骨髓基质细胞的胞质内出现细小的蓝色铁颗粒。

The open technique included open removal of the broken nail, reinsertion of a stable intramedullary nail or plate, and cancellous bone graft supplementation.

开放技术包括开放取出髓内钉，重新置入稳定的髓内钉或钢板，并取松质骨植骨加强。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。