内质的

The colloid in follicles with low activity usually shows more intense eosinophilia compared with colloid in follicles with a high level of hormone synthesis and release.

功能较弱滤泡内的胶质与激素合成和释放增多滤泡内胶质相比，通常显示更强的嗜伊红性。

We also have observed some organs pathological changes of juvenile Jian Carp, such as pancreas acinous gland and zymogen granules of acinous gland cell disappearing, intercellular substance hyperplasia and inflammatory cells soakage, hepatatrophia, liver cell granular or vacuolar degeneration and necrosis, karyolysis or pyknosis, glycogen granules decreasing, metanephros atrophy, metanephric canaliculus epithelium granular or vacuolar degeneration and necrosis, mitochondrion swelling and mitochondrion cristae disappearing, karyolysis, distal convolutal tubule microvilli desquamating, spleen marrow cell degeneration and necrosis, intercellular substance of spleen hyperplasia, spleen atresia, blood corpuscle disappearing.

后肾土黄色、淡褐色或苍白色，肾小管上皮细胞肿胀、颗粒或水泡变性、坏死，细胞内有大量血细胞流出，线粒体肿胀，嵴结构消失，细胞核溶解，肾间质甲状腺滤泡和拟淋巴细胞增生，远曲小管微绒毛脱落、管道细胞界限不清。心脏肌纤维肿胀、颗粒或空泡变性，严重的肌纤维溶解、变细或断裂，肌纤维间水肿、炎性细胞浸润，部分心肌细胞核浓缩。脾脏暗褐色，脾髓质细胞变性、坏死，拟淋巴细胞明显减少，淋巴细胞岛少见、岛中细胞成份减少，黑素巨噬细胞中心减少、体积缩小，脾脏网状基质水肿，脾窦闭锁，血细胞减少。

Adipocytes in bone marrow cavity could decrease the activity of ALP, weaken the expression of Collagen Ⅰ mRNA, inhabit osteogenic potential of bone marrow stromal cell, and induce apoptosis of bone marrow stromal cells.

髓内脂肪细胞可降低骨髓基质细胞内ALP活性，减弱CollagenⅠ mRNA表达，干扰其成骨能力，并能诱导骨髓基质细胞发生凋亡，在骨质疏松症的发病中有着重要的意义。

The N protein concentration in the supernatants of virus-infected cells were reduced at different levels in all ER and cytoplasma localized intracellular antibody expressing cell lines, however, the intracellular N protein synthesis in above cell lines was not significantly affected.

利用病毒微量滴定免疫荧光方法，对细胞内抗体与病毒的增殖关系进行了研究，证实无论在内质网还是在胞质，抗核蛋白细胞内抗体都能够抑制病毒的增殖，具有抗病毒活性。

The results show:(1) In testis, PNA receptors sites were firstly observed in the cytoplasm and plasma membrane of primary spermatocyte, and greatly increased in the plasma membrane of second spermatocyte, then decreased and were accumulated in the tail of the round spermatid. The same reaction was observed in sperms of testis and spermatheca, which shows stronger reaction in head and weaker in tail. A universal dyeing was observed on the plasma membrane of sertoli cells.

结果表明：(1)在中华蚱蜢的精子发生中，PNA受体是由初级精母细胞合成，然后转移到膜上，在次级精母细胞，由于量的积累其质膜出现了强阳性标记；从早期精子细胞到成熟精子时期糖蛋白的分布发生了明显的修饰变化，这些变化与精子获得与卵子进行识别、粘附、结合等受精能力密切相关：受精囊内与精巢内的精子表面的标记是相同的，说明质膜糖蛋白的修饰作用在精巢内就已完成。

Cytomixis was observed at synizesis of prophase Ⅰ in this experiment, and MN in cytoplasm and in nucleus was noticed to distribute in these granules which migrated from one PMC into its neighboring cell.

在凝线期的花粉母细胞中观察到细胞融合现象，有胞质或核内微梁骨架与穿壁转移的胞质小球和核小球内骨架相连。

Positive staining of oxytocin and vasopressin were in the cytosol of the neurons and the former gave less intensity,many positive varicosities for vasopressin were also noticed in neurite.

催产素的免疫阳性产物主要在细胞核周围的胞浆即核周质内，而加压素的免疫阳性物质除了在核周质内有很强的反应外，在突起内也可见很强的免疫反应。

The reservoir rock types in the region of interest are grain limestone, algal limestone, cryptite, siltstone, muddy limestone and mudstone. The thesis identified as a result of changes in the deposition causing the heterogeneity in plane, and then the geological oil reserves have been calculated in accordance with the areal heterogeneity.3、Based on the data of core analysis, bore logging, array induction imaging log and FMI, porosity and permeability model were built using regression analysis. Through testing 45 rock samples, a, b, m, n value were obtained and used to calculate single-layer oil saturation value using the Archie formula. According to the theory of laterolog and inductolog, resistivity ratio and cross plot were used to identification effective thickness. Effective thickness was identified delicately in 103 wells. Based on the over work, active porosity and oil saturation was calculated accurately.4、According to observation of 100 pieces of the thin slices, cast thin slices and 383m core in three wells, the chief diagenesis in this area were compaction, cementation, denudation and cataclasis. Cementation and denudation were universally influenced active porosity this area. The intergranular pores, residual pores and secondary pores were formed in thin carbonated reservoir of lacustrine facies.5、The influence of reservoir heterogeneity to reservoir calculation was analyzed systematically using actual data. And the conclusions were as follows:①The principal reason leading to oil distribution unequal is interior structural feature of reservoir in structural hydrocarbon reservoir.

确定了由于沉积微相的变化而导致的平面非均质性，进而在平面上按照平面非均质单元计算了石油地质储量。3、利用岩心分析、常规测井曲线与阵列感应、成像测井资料相结合，分岩性利用回归分析方法建立了储层孔隙度、渗透率模型；通过对45块岩样进行岩电试验，获取了a、b、m、n值，利用阿尔奇公式计算了各单层含油饱和度值；根据侧向电阻率和感应电阻率串并联特性，利用电阻率比值法与常规交会图法相结合重建了有效厚度图版，对全区103口井目的层段进行了有效厚度精细划分，通过以上工作，准确求取了储量计算单元内有效厚度参数、有效孔隙度参数以及含油饱和度参数。4、通过对研究区3口井383m岩心观察，100余块薄片、铸体薄片镜下鉴定得出：油泉子油田上、下油砂山组储层主要成岩作用是压实作用、胶结作用、溶蚀作用和破裂作用，其中溶蚀作用、胶结作用最普遍，这两种作用对有效孔隙度影响最大，以上成岩作用形成了原生粒间孔、粒间残余孔和次生孔隙(如粒间溶孔、粒内溶孔、铸模孔、晶间孔等)为主的薄层湖相碳酸盐岩储层。5、利用实际资料，系统分析了油藏非均质对储量计算的影响，认为：①对于构造油气藏而言，油气藏构造及断裂特征是造成油气分布不均的首要原因，储集条件相同的条件下，构造高部位有利于储存油气，油气在垂向的重力分异体现了油气藏内部构造的不均一特征。

Using maximum flooding surface and local explosure erosion surface as the correlation framework surface in the 4-th hierarchical base-level cycles, sand-body in the 3-th hierarchical base-level cycles formed in the 4-th hierarchical base-level cycles may be correlated layer by layer. The law of the sand-body and the characters of reservoir heterogeneity formed in the 4-th hierarchical base-level cycles are discussed. The study shows:①During early period of the 4-th hierarchical base-level cycle rising, subaqueous distributary channel formed, continuously stacked vertically, and migrated in limit range laterally, and channel sand-body stretches farther longitudinally, and lacks interbeds of mud rock and siltsand in sand rock, reservoir heterogeneity is relatively weak.②During mid-period of the 4-th hierarchical base-level cycle rising, subsaqueous distributary channel migrated actively, channel divided and converged strongly to form netted sand-body and sand sheet mainly, but interbeds of mud rock and siltsand increases gradually in sand-body, reservoir heterogeneity increased.③During later period of the 4-th hierarchical base-level rising and early period of the 4-th hierarchical base-level falling, reservoir sand-body was poor, and only form thin sand-body of subsaqueous distributary channel, subsaqeuous creveas and distal mouth bar, sand-bodys are surrounded by mud and silt rock.

进一步采用以第4层次基准面旋回中相当最大湖泛面的相转换面和具有区域性暴露侵蚀作用的层序边界面为等时地层对比框架的边界，对发育于MSC2—MSC4三个第4层次基准面旋回的第3层次基准面旋回内砂体进行了逐层等时对比，探讨第4层次等时地层对比格架内储集砂体发育分布规律，及储集砂体的非均质性特点，研究发现：①第4层次基准面上升初期发育的水下分流河道，砂体呈连续叠置、侧向迁移受限，纵向上呈长距离延伸的带状产出，砂体间泥、粉砂岩夹层少，储层非均质性相对较弱；②在第4层次基准面上升中期发育的水下分流河道侧向迁移活跃，纵向延伸过程中分流汇合作用强烈，以形成网状或席状连片砂体为主，但砂体间泥、粉砂岩夹层逐渐增多，储层非均质性增强；③第4层次基准面上升晚期和下降早期，不利于储集砂体发育，仅出现少量薄的水下分流河道、水下决口扇、远—河口砂坝砂体。

The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法，研究丘脑网状核(nucleus reticularis thalami，RT)中γ-氨基丁酸(gamma-amino butyric acid ，GABA)能神经元、一氧化氮(nitrogen monoxidum，NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate，cAMP)及中缝背核(nucleus raphes dorsalis，DRN)至RT的5-羟色胺(5-hydroxytryptamine，5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP，5μg)，注射当天睡眠时间略有减少，第二日睡眠时间显著减少，觉醒时间明显增多，第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后，与生理盐水组比较，睡眠时间增加，觉醒时间减少；而RT内微量注射L-谷氨酸(glutamic acid， L-Glu， 0.2μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline，BIC，1.0μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen，BAC，1.0μg)后，产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg，0.5μg)后，与生理盐水组对比，睡眠时间略有减少，但无显著性意义；而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside，SNP，0.2μg)后可明显增加觉醒时间，缩短睡眠时间；微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine，L-NNA，2.0μg)后，引起睡眠时间增多，觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用；RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后，与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠，其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate，MOR，1.0μg)后与生理盐水组对比，睡眠时间减少而觉醒时间增加； RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride，NAL，1.0μg)后与生理盐水组比较，睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后，与生理盐水组对比，原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较，睡眠时间增多而觉醒时间减少；RT内微量注射亚甲蓝(methylene blue，MB，1.0μg)后，与NS组比较，睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 0.2μg)比较，睡眠时间减少，觉醒时间增多；大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射二甲基麦角新碱(methysergide， MS， 1.0μg )后，与对照组(DRN注射L-Glu 0.2μg，双侧RT注射NS 1.0μg)比较，睡眠时间增多，觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine，PCPA，10μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 1.0μg)比较，睡眠时间增多，觉醒时间减少；大鼠DRN内微量注射PCPA(10μg)，产生睡眠增多效应后，在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan ， 5-HTP， 1.0μg )后，与对照组(DRN注射PCPA 10μg，双侧RT注射NS 1.0μg)比较，睡眠时间减少，觉醒时间增多。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。