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The patterns of amyloplast proliferation behaved "amyloplast envelop dilating, evaginating, and budding to form new amyloplast","amyloplast envelop constricting to form new amyloplast","form new amyloplast as the middle clapboard" and "amyloplast envelope dilating and invaginating to form new amyloplast".

玉米胚乳细胞淀粉质体增殖有"被膜出泡、外凸,以出芽方式形成淀粉质体"、"淀粉质体被膜缢缩形成淀粉质体"、"以中间隔板形式形成淀粉质体"及"淀粉质体被膜向内出泡,在淀粉质体内形成新的淀粉质体"等几种方式。

The patterns of amyloplast proliferation behaved "amyloplast envelop dilating, evaginating, and budding to form new amyloplast","amyloplast envelop constricting to form new amyloplast","form new amyloplast as the middle clapboard" and "amyloplast envelope dilating and invigilating to form new amyloplast".

玉米胚乳细胞淀粉质体增殖有被膜出泡、外凸,以出芽方式形成淀粉质体;淀粉质体被膜缢缩形成淀粉质体;以中间隔板形式形成淀粉质体;淀粉质体被膜向内出泡,在淀粉质体内形成新的淀粉质体等几种方式。

The double membrane vesicle, which was produced by budding of the outer membrane of amyloplast, accumulated starch to form new amyloplast. The double membranes of amyloplast invaginated and the inner membrane dilated to form new amyloplast in the amyloplast.

淀粉质体增殖产生新淀粉质体有多种方式:出芽增殖、缢缩增殖、中间隔板增殖、被膜向内出泡或内陷增殖、被膜形成双层膜小泡再积累淀粉增殖,它们均是淀粉质体被膜的一种膜行为。

Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有按摩明显的阻抑作用,加药48h后增殖的抑制率可达43%以上;(2)ATP处理的U937细胞周期发生改变,G1期细胞数按摩明显增多,ATP作用24h时G1期前出现亚二倍体峰——凋亡峰,凋亡细胞数为3.4%,作用48h时凋亡细胞数增多为22.7%;(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜,逐渐向核膜外移动,进入胞浆内再移向质膜内外面,紧贴质膜外面再逐步脱离细胞体,进入细胞基质中,成为游离的凋亡小体。

Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used. Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有明显的阻抑作用,加药48h后增殖的抑制率可达43%以上;(2)ATP处理的U937细胞周期发生改变,G1期细胞数明显增多,ATP作用24h时G1期前出现亚二倍体峰——凋亡峰,凋亡细胞数为3.4%,作用48h时凋亡细胞数增多为22.7%;(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜,逐渐向核膜外移动,进入胞浆内再移向质膜内外面,紧贴质膜外面再逐步脱离细胞体,进入细胞基质中,成为游离的凋亡小体。

Thirdly,The nuclear chromatin of lung carcinoma cells concentrate to periphery, the electron density of cytoplasm increased, in some cells,the heterochromatin of nucleus were blurry,and the nucleole became uniform in electron density, the formatio reticularis of nucleole disappeared.

细胞核的改变:细胞核内染色质边集,基质电子密度增高。有些细胞核内异染色质分界不清,核仁呈均一电子密度,网状结构消失。

Results Among the three groups,the children's rib cartilage had the most blood vessels,the most chondrocytes,well-distributed stain of matrixes,and the type Ⅱ collagen was expressed actively and highest in photedensity.The rib cartilage of teenager group had less blood vessels,unhomogeny distributed stain of matrixes,the enlarged and separated cartilage lacunas.The rib cartilage in adult group showed the least blood vessels,the least chondrocytes.the hyalinization of perichondium,the most deposition of calcium salt,and the type II collagen was expressed at the lowest level in photodensity.

结果 儿童组肋软骨膜血管最丰富,软骨基质染色均匀,软骨细胞数目最多,Ⅱ型胶原蛋白表达最活跃,平均积分光密度值最高;青少年组软骨膜内血管减少,软骨基质染色出现明显的不均质状,软骨陷窝体积变大,并呈分隔状,陷窝内软骨细胞数目减少,II型胶原蛋白表达较儿童组减弱;成人组软骨膜血管、细胞成分明显减少,软骨膜内的纤维成分明显玻璃样变,钙盐沉积较青少年组时明显增多,Ⅱ型胶原蛋白表达较青少年组减弱。

After 45 days of having tissued, the record showed that the three parts flake of L.davidiivar Cotton did not have the same differentiation ability in the same matrices.The alive rate in each matrices is: the middle is the best, the outer is the worst and the innner is better.

培养45天时统计了各种基质中的成苗情况,结果表明,在16种基质中,每一种基质都证明了兰州百合内、中、外三层鳞片在同一基质中的分化能力不同,每一种基质中中层鳞片成苗率最高,内层次之,外层最差。

The patterns of amyloplast proliferation behaved "amyloplast envelop dilating, evaginating, and budding to form new amyloplast","amyloplast envelop constricting to form new amyloplast","form new amyloplast as the middle clapboard" and "amyloplast envelope dilating and invaginating to form new amyloplast".

玉米胚乳细胞淀粉质体增殖有&被膜出泡、外凸,以出芽方式形成淀粉质体&、&淀粉质体被膜缢缩形成淀粉质体&、&以中间隔板形式形成淀粉质体&及&淀粉质体被膜向内出泡,在淀粉质体内形成新的淀粉质体&等几种方式。

The result using inhibitors of Ca〓 channels supported above observation. When Ca〓 channels in plasma membrane were blocked by LaCl〓, verapamil or nifedipine, and the Ca〓 channels in endomembranes opened naturally, extracellular CaM couldn't induce 〓 increase. When Ca〓 channels in endomembranes were blocked by ruthenium red and the Ca〓 channels in plasma membrane opened naturally, extracellular CaM induced Ca〓 increase in guard cells, but the extent of Ca〓 increase was lower than that of control.

使用质膜Ca〓通道抑制剂LaCl〓、异博定、尼群地平及细胞内Ca〓通道抑制剂钌红的结果支持这种推测:在质膜Ca〓通道被阻断、细胞内Ca〓通道仍正常开放的情况下,保卫细胞〓不能被CaM诱导增加;细胞内Ca〓通道被抑制、质膜Ca〓通道正常开放的条件下,细胞外CaM诱导保卫细胞内〓升高的幅度不能达到不使用Ca〓通道抑制剂的水平。

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