内质体

In the normal group, electron microscopy showed normal chondrocytes were oval, and cells and cell membrane were intact. In cytoplasm, there were abundant rough endoplasmic reticulum, dictyosome, and mitochondria with intact nucleus, and even chromatin. In the model group, chondrocytes exhibited obvious pyknosis and their shape was irregular. The areolae around cells disappeared; cell organs in cytoplasm coagulated to flaps which showed electron dense and was uneasy to observe; the shape of nucleus showed irregularity, and caryotin gathered densely.

电镜下正常对照组软骨细胞呈椭圆形，细胞及胞膜完整，包质内可见丰富的粗面内质网、高尔基体、线粒体，细胞核完整，染色质分布均匀；模型对照组软骨细胞明显固缩且外形不规则，细胞周晕消失，胞浆内细胞器凝成高电子密度的片状物不易分辨，细胞核不规则，染色质浓聚，散裂于核中。

It was also found that at experimental concentrations there was no regularity for the change of activity of copper salicylate encapsulated in negatively charged liposomes, and copper salicylate encapsulated in liposomes with more negative charges could promote the dismutation of superoxide anion free radicals, however, so could copper salicylate encapsulated in liposomes with fewer negative charges only in lower concentrations.

并发现在实验浓度范围内，负电性脂质体包裹的水杨酸铜，活性变化无规则，且荷负电量多的脂质体包裹的水杨酸铜促进超氧自由基的歧化，而荷负电量少的脂质体仅在低浓度区对超氧的歧化表现为促进作用。

Result:After removal of the bilateral lower molars, the remodeling and degenerative activities in different layers of the condyles increased, such as vesiculation of endoplasmic reticulum, distortion of mitochondria and increase of lysosome. Furthermore, the sequential arrangement of collagen fibrils was disturbed, the collagen network broke down and the proteoglycan granules decreased.

结果：细胞发生了不同程度的改建及退行性反应，如粗面内质网的扩张，线粒体肿胀，溶酶体增多等；基质中胶原原纤维的有序排列结构消失，胶原网受到破坏，同时蛋白多糖颗粒减少；基质中脂质小体及基质小囊等成分增多；负重区较非负重区的改变更显著。

HRP was coupled with the fluorochrome FITC and encapsulated with liposome . pEGFP-N1 plasmid (expressing green fluorescent protein) with encapsulation in commercially available liposome was prepared. The eyes were enucleated 1, 4 and 8 weeks after zonule rupture and anterior segments comprising lenses were incubated in medium containing one of these components. Cryo-sections were made and translocation of fluorescent macromolecule from the medium into the lens and green fluorescent protein expression in the epithelium were observed by fluorescent microscopy.

制备FITC标记的HRP脂质体；制备pEGFP-N1质粒（表达绿色荧光蛋白的质粒），并用脂质体包裹；豚鼠悬韧带部分离断后1周、4周、8周取含晶状体的眼前段标本分别在含有FITC-HRP复合物、pEGFP-N1质粒的培养基中孵育，冰冻切片，荧光显微镜观察FITC-HRP复合物进入晶状体和pEGFP-N1质粒在晶状体内表达的情况，比较悬韧带离断侧与非离断侧的差异。3。

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果：倒置显微镜和透射电镜观察显示，经SAHA处理的细胞增殖速度显著减慢，细胞体积增大，细胞核较小，形状较为规则，核仁数量减少、体积变小，核内常染色质增多而异染色质减少，核质比例减小，细胞质内线粒体数量增多、线粒体嵴发达，高尔基体较为典型，粗糙型内质网增多，呈现出与正常上皮细胞相似的形态变化；MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用，并有明显的剂量依赖和时间依赖关系；免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达；流式细胞仪检测结果显示，SMMC-7721细胞经SAHA处理后，G0/G1期细胞明显增加，S期细胞则明显减少，细胞被阻滞于G0/G1期；RT-PCR检测结果表明，SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加，24h后更为明显。

The results showed that ACP positive reactions were mainly deposited in lysosome, nucellar cell, cellular endomembrane, mitochondriurn, and endoplasmic reticulum in four tissues of the healthy shrimp. Furthermore, ACP activity was located in microvilli, and around partly lipid droplet in liver. AKP positive reactions were mainly deposited in cell membrane, nucellar cell, lysosome. endoplasmic reticulum and cellular endomembrane in four tissues.

结果显示，在健康的对虾体内，ACP依次出现在各组织中的溶酶体、细胞核、细胞内膜、线粒体、内质网以及肝脏组织中的部分脂滴周围及微绒毛中AKP依次出现在各组织中的细胞膜、细胞核、溶酶体、内质网以及肝脏组织中的脂滴周围及微绒毛中。

Observed with transmission electronic microscope,ES-like cell have obvious nucleolus and a little heterochromatin,and many ribosomes in the cytoplasm with much endoplasmic reticulum and mitochondria. A part of cells appeared alveoli on the nuclear envelope,and chromatin was pycnosis and the .

在透射电镜下细胞核仁明显、核异染色质少；细胞质中核糖体较多，内质网、线粒体发达，部分细胞的细胞核膜上出现小泡，染色质边集，染色质形成凋亡小体。

However, the membrane of toxopalsma in liver tissues was stickled out and broken, vacuole lied in cytoplasma, mitochondrion and endoplasic reticulum were swollen or broken, and dense granules were disappearing TEM ×10000

肝组织内弓形虫随扁桃酸作用时间的延长，其受损程度明显加重。在治疗早期(24、72h)肝组织内虫体胞膜突出、破裂，胞质中出现空泡，线粒体和内质网肿胀或破裂，致密颗粒消失，虫体电子密度降低(图2B)。

RESULTS: In the verapamil group, the fibroblast were round or oval, and there were tiny cell process, less alpha substance, atrophic and decreased rough endoplasmic reticulum in kytoplasm and abundant vesica. In control group, there were fusiform or polygon fibroblast, and much matrix among cells, abundant rough endoplasmic reticulum and ribosome in cytoplasm and less vesica were found.

结果：维拉帕米组神经瘢痕成纤维细胞呈球形或椭圆形，细胞突起细小，胞间网状物质少，胞浆中粗面内质网萎缩且数量减少，囊泡丰富；对照组成纤维细胞呈梭形或多角形，细胞间基质较多，细胞浆内粗面内质网及核糖体丰富，囊泡少见。

The cell in acidic situation presented a degenerative chang-ment,such as an increased lipid drop and empty cavity in the cell plasma,a py-knotic nuclei,the dilatation of the endoplasmic reticulum and Golgi saccules,mi-tochondrial swelling the number of ribose decrease,and the ribose dissociated inthe plasma,a lot of swallowing cavity,the fibric material decreased accompany-ing with the acid being stronger.

随培养基pH逐渐下降，上述过程因受酸性阻抑而延缓，细胞增殖速度也受影响，处于酸性环境中的软骨细胞呈退行性变化，表现为细胞浆内脂质颗粒与小泡增多、细胞核皱缩、核仁浓染、内质网线粒体膨胀、核糖体数目减少，且与粗面内质网结合的少，游离在胞浆中的多。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。