内质体

In the brain of adult rat, the positive immunohistochemical product of lSL-l (ISL-l-positive) was mainly located in the neuronal nucleus and found in discrete regions except to brain cortex, such as the Purkinje cell layer and the granular cell layer of cerebellum, the granular cell layer and the pyramidal cell layer of hippocampus, the mitral cell layer, the internal and external plexiform layer, the granular cell layer and the granular cell layer of olfactory bulb and so on, and several nuclei of the hypothalamus, midbrain and pons, such as claustrum, anterior olfactory nucleus, accumbens nucleus, caudate-ptamen, pallidum, substantia nigra, striatum, islands of Callaje, mammillary nucleus, anterior pretactal nucleus, habenular nucleus, amygdaloid nucleus, cuneate nucleus, rubral nucleus, gigantocellular reticular nucleus and so on.

在正常成年大鼠脑中，同源框基因islet-1表达产物（ISL-1）免疫组织化学阳性物质广泛分布于除大脑皮层外的神经细胞的细胞核内，ISL-1阳性神经元密集分布于小脑Purkinje细胞层和颗粒细胞层、海马的颗粒细胞层和锥体细胞层、嗅球的内丛层、外丛层、颗粒细胞层及僧帽细胞层等，另外在丘脑、中脑和桥脑的一些重要神经元核团均有分布，如，屏状核、前嗅核、伏核、尾壳核、苍白球、黑质、纹状体、Calleja岛、乳头体核、前顶盖前核、缰核、杏仁核、楔束核、红核网状巨细胞核等。

The thylakoid of chloroplast was lost, the chloroplast was shrinked and composed into several discontinuous parts, which suggest that the function of chloroplast was destroyed;(2) the cell wall was thickened clearly, plasmolysis was found in most of Platymanas subcordiformis cells;(3) the number of mitochondria increased, some cristae of mitochondria were dissolved;(4) the number of vacuolar increased and the size of vacuolar was not uniform, the shape was deformed;(5) the nucleus was deformed and part of the nucleus membrane was dissolved;(6) Golgi dicytosomes accompanied with a loose arrangement of cisternae and the peripheral tips of the dictyosome cisternae are not especially swollen.

叶绿体内的类囊体缺失，叶绿体皱缩，叶绿体被分离成几个不连续的部分，说明叶绿体功能被破坏；(2)细胞壁明显增厚，细胞壁与细胞膜存在质壁分离现象；(3)线粒体数目增多，部分内嵴遭破坏；(4)液泡数目增多，大小不等，形状不规则；(5)细胞核变形，核膜部分解体；(6)UV-B处理的高尔基体有一个排列松弛的潴泡，潴泡的外围顶端并没有特别膨大。

Poptosis-inducing factor is a mitochondrion-localized flavoprotein with NADH oxidoreductase activity, which is encoded by the nuclear gene. Following induction of apoptosis, AIF is released from mitochondria and translocated to cytosol, and then to the nucleus. The mechanism of AIF translocation from mitochondria to nucleus is still unclear. It has been suggested that translocation is due to the presence of nuclear localization signal.

bstract： AIF(apoptosis-inducing factor)为位在粒线体内膜膜上之蛋白质，在正常细胞中，能够藉由维持电子传递链复合体I的稳定性，进而影响细胞能量的产生及自由基的清除，并且也会维持粒线体的构造；反之，当细胞遭受到不当刺激时，粒线体膜通透性改变，使得原本存在於粒腺体间质中之AIF，被释放出来，进入细胞核内造成DNA片段化，进一步促进细胞凋亡。

Neuronal cell bodies and fibers with moderate mGluR7-LI were seen in the olfactory bulb,anterior olfactory nucleus,piriform cortex,septofimbrial nucleus,bed nucleus of the strial terminalis,lateral hypothalamic area,paraventricular hypothalamic nucleus,supramammillary nucleus,medial and lateral mammillary nuclei,most part of the thalamus,medial and lateral geniculate bodies,nucleus of the optic tract,red nucleus,substantia nigra,interpeduncular nucleus,pontine nuclei,lateral parabrachial nucleus,superior olivary complex,nucleus of the trapezoid body,motor nucleus of the trigeminal nerve,facial nucleus,ambiguus nucleus,cochlear nucleus,vestibular nuclei,nucleus of the solitary tract,hypoglossal nucleus,prepositus hypoglossal nucleus,medullary reticular formation,Purkinje cells of the cerebellum,nucleus raphe obscurus,intermediolateral nucleus of the spinal cord,Onuf′s nucleus and lamina X of the spinal cord.

呈中等强度染色的神经元胞体和纤维见于嗅结节、前嗅核、梨状皮质、隔伞核、终纹床核、下丘脑外侧区、下丘脑室旁核、乳头体上核、乳头体内、外核、丘脑大部分核团、内外侧膝状体、视束核、红核、黑质、脚间核、桥核、臂旁外侧核、上橄榄复合体、斜方体核、三叉神经运动核、面神经核、疑核、耳蜗核、前庭核簇、楔束外核、孤束核、舌下神经核、舌下神经前置核、中缝隐核、延髓网状结构、小脑蒲肯野细胞层、脊髓中间带外侧核、Onuf核和中央管周围灰质。

The porine is the polyembryony animal, many obtains the ovocyte from the porine ovary although but the maturity quite to be low; Because the ROSI technology is does not have to grow the mature sole round sperm cell to pour into directly completely in the ovicell nature, jumped over the spermatozoon in to pass through the physiology and the biochemistry, if the ovicell nature mature or the activation degree were insufficient, added the round immature sperm cell maturity quite inferior reason, very possibly Causes the ROSI micro fertilization defeat.

猪是多胎动物，从猪卵巢上获得的卵母细胞数量虽然较多，但成熟度比较低；由于ROSI技术是将没有完全发育成熟的单一圆形精细胞直接注入卵胞质内，跳越了精子在穿过透明带和卵质膜等过程中所发生的生理和生化反应；如果卵胞质成熟或活化程度不够，加之圆形未成熟精细胞成熟度比较差等原因，很可能造成ROSI受精的卵母细胞内部激活因子蓄积减少和生成不足，引起精核解聚困难、精圆核不能形成、第二极体不能排出、卵母细胞孤雌发育率增加等，使ROSI显微受精失败。

Result 1 Magnetic nanoparticles, magnetic nanoparticles modified with antisense oligodeoxynucleotide of human telomerase reverse transcriptase induced HL-60 tumor cells to apoptosis, we could see typical morphologic change of apoptosis cells: karyopyknosis, chromation"s condensing and aggregation in nuclear, forming crescent-shaped or annulus structures to lean on edge of cell nucleus"s membrane and posing apoptosis body by Atomic Force Microscope, Fluorescence microscope, transmission electron Microscope 2 There was a significant difference compared with control group(p.01), inhibition ratio had significant positive correlation with medication dosage and time ;during 0.8-8μM dosage amplitude, inhibition ratio accrescenced by dosages increasing. However, the inhibition ratio would decrease when dosage over 8-80μM.

结果 1 磁性纳米粒子、修饰有端粒酶反义寡核苷酸的磁性纳米粒子诱导HL-60细胞发生凋亡，原子力显微镜、光学显微镜、荧光显微镜和透射电镜下均观察到HL-60细胞呈现典型的凋亡细胞的形态变化：细胞核固缩，核内染色质浓缩、凝聚、形成新月形或环状结构紧靠在细胞核膜边缘，并形成凋亡小体。2 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子对HL-60肿瘤细胞的生长和增殖有明显的抑制作用，与对照组相比有显著性差异(p＜0.01)，在剂量为0.8-8μmol／L范围内，抑制率随剂量的增加而增加，当剂量超过8μmol／L时，抑制率反而下降；3 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子可增强p53基因的表达活性，引起DNA降解损伤，反向调节细胞周期活动，促使细胞从G0期进入G1期，抑制肿瘤细胞的生长。4 修饰有端粒酶反义寡聚脱氧核苷酸的量子点能通过内吞作用进入HL-60肿瘤细胞的细胞核，可以在细胞内进行定位和促进HL-60肿瘤细胞的凋亡。

Substance P distributes nonuniformly in the striatum and globus pallidus.The postnatal development of this neurotransmitter has significant difference with other neurotransmitters in the brain.

P物质非均匀性地分布在纹状体和苍白球内，纹状体和苍白球内P物质的生后发育与其他神经递质有显著的差异。

Results C/EBPαand C/EBPβexpressed differently in the outer root sheath,follicular papilla,hair matrix and sebaceous gland in embryonic period.The positive signals were chiefly distributed in the nucleˉuses.The expression of C/EBPαand C/EBPβwere found in the basal cell layer of outer root sheath and sebaceous gland in the new born and maturation phase,The expression in nucleuses of outer root sheat cells decreased with the differentiation but enhanced oppositely in the cytoplasm.

结果 C/EBPα和C/EBPβ分别在胚胎期毛囊外根鞘，毛乳头，毛母质及皮脂腺细胞的胞核内强表达；新生儿毛囊峡部下端外根鞘基底层细胞的胞核内也存在强表达，基底内层胞核阳性信号逐渐减弱；而成体毛囊核内阳性信号仅定位于峡部下端外根鞘基底层及皮脂腺基底层。

Aimed at the complexity of fluvial strata, such measures as well cyclical comparison, step control and geological analysis of reference beds have been applied. Moreover the paper has developed a set of methods the techniques to extract the parameters controlling the formation of intra-sandbody thin interbeds. The present study has examined how thin interbeds affect the frame property and heterogeneity of individual sandbodies; revealed the permeability space distribution mode of meandering distributary channel's single side lithosomic body and straight distributary channel's aggrading lithosomic body. Through applying the distributary channel sandbody structural unit classifying and identifying method achievement, some horizontal wells have been deployed to dig the residual oil in the top of hick pay zone in the xing8-9 area, which shows better effect after they have been put into production.

针对分流河流砂体沉积特点采用了旋回对比、分级控制、不同相带区别对待的单油层对比方法；利用取心井、对子井等资料研究形成了分流河道砂体内部结构单元的倾角、倾向、规模等参数的提取方法；搞清了内部结构单元对单一砂体内非均质性的控制作用，建立了分流平原相单一点坝侧积体及顺直型分流河道单一加积体层内渗透率分布模式及剩余油分布模式，应用分流河道砂体结构单元划分方法识别研究成果，在杏树岗油田杏八九区纯油区部署了水平井，投产后见到了较好的厚油层顶部剩余油挖潜效果。

The results showed compared with the native state the shear strength of rockmass under the saturation state decreased by 11.17%, cohesion force decreased by 16.09%, internal frictional angle decreased by 10.02%.(3) 3D-σFEM numerical simulation software is used to simulate the slope stability on the native state and saturation state. Compared with the nature state the displacement of the discontinuity and stress concentration in the saturation state is largened clearly, the results showed slope stability under the rainfall is weakened.

更多含有天然结构面的岩体试件进行了不同含水量下的强度试验，试验结果证明在岩体试件处于饱水状态时的结构面抗剪强度比天然状态下平均下降了11.17%、粘聚力平均下降了16.09%、内摩擦角平均下降了10.02%；(3)采用3D-σ有限元数值软件对K86+180～K86+470段岩质边坡进行了三维有限元数值模拟，并且分天然状态分阶段开挖和饱水状态分阶段开挖两种不同状况进行了稳定性分析，从分析结果可以看出与天然状态相比，饱水状态下软弱层水平方向位移明显增大，坡脚及软弱层特别是软弱层临空面附近的剪应力值显著增大，并且其安全系数与天然状态相比也显著减小，说明在有大的降雨发生当岩体处于饱水状态时边坡整体稳定性被削弱，边坡失稳可能性增大。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。