内质体

Results The amaranthine nuclei had a distinct outline and distributed evenly on the slide. The area of nuclei in liquidbased preparation was much smaller and chromatin was more condensed in comparison with those in conventional smear. The nuclei in conventional smears had the largest value of area and the value of AOD, and CV of IOD, the IOD ratio among diploid, tetraploid and octaploid DNA was most close to 2 and 4, and the CV of IOD exceeded 6%.

结果 三种涂片内肝细胞核分布均匀，轮廓清晰，呈紫红色，与传统涂片法相比，液基法内肝细胞核面积明显减小，染色质高度浓缩；相同倍体的肝细胞核在传统涂片中面积最大，平均光密度最低，各倍体间的比值最接近2和4，积分光密度CV值最低（。5）。

Under the electronic microscope,lamellar body and cellular organs such as bioblast in endochylema of the four treatment groups were more obvious compared with the control group.

透射电镜观察，地塞米松治疗组Ⅱ型肺泡上皮内多见板层小体且染色深？致密；胞质内线粒体等细胞器多见，而生理盐水组内难见板层小体？

Under the electronic microscope, lamellar body and cellular organs such as bioblast in endochylema of the two treatment groups were more common compared with the control group. And lamellar body in the two treatment groups was more anachromasis and condensed compared with the control group.

透射电镜观察，沐舒坦治疗组、地塞米松治疗组Ⅱ型肺泡上皮内多见板层小体且染色深、致密，胞质内线粒体等细胞器多见，而对照组内难见板层小体、少见细胞器。

Purposes: To observe intakes of 〓p-sodium-orthophosphate and 〓P-chromium- orthophosphate at various concentrations by brain gliomas cell; To make thorough inquiry of the inhibition to the SHG44 cell by 〓P-sodium-orthophosphate and 〓P-chromium-orthophosphate at various concentrations; To find out the regularities about the accumulation and elimination in the major organs of rats after inter-brain injection with 〓P-sodium-orthophosphate and 〓P-chromic-orthophosphate; To investigate the therapeutic effects of intert-injection with 〓p-chromium-orthophosphate on the neoplasia transplanted in the brain and hypoderma; To estimate the absorbed dose of the neoplasia body in rats after internal radiation; To study the pathological changes in the rat cells and tissues caused by the radiation exposure from 〓p.

目的：观察人脑胶质瘤SHG44细胞对不同浓度的〓P-磷酸钠和〓P-磷酸铬的摄取；探讨不同浓度〓p-磷酸钠和〓p-磷酸铬对SHG44细胞生长的抑制；揭示〓p-磷酸钠和〓p-磷酸铬在大鼠颅内注射在时全身主要脏器的蓄积与排除规律；观察〓p-磷酸铬大鼠颅内瘤体、皮下瘤体内注射的治疗效果；估算大鼠瘤体组织的吸收剂量；观察〓p照射引起的细胞及组织的病理学改变，为临床脑胶质瘤内照射治疗提供实验依据和吸收剂量计算方法。

The typical apoptotic morphological features appeared in MUTZ1 cells treated with 4 mmol/L VPA for 72 hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dosedependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dosedependent manner, the cells were arrested at G0/G1 phase.

结果显示： VPA对MUTZ1细胞的生长抑制作用呈现时间和剂量依赖性；经4 mmol/L VPA处理MUTZ1细胞72小时后，细胞呈现典型的凋亡形态特征，光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体；透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加，胞浆内大小不规则的染色质团块；流式细胞术结果表明，细胞凋亡率随着VPA浓度的增加而逐步增高，G0/G1期细胞比例随着VPA浓度的增加而逐渐增多，S期细胞比例逐渐减低，细胞被阻滞在G0/G1期。

The typical apoptotic morphological features appeared in MUTZ-1 cells treated with 4mmol/L VPA for 72hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dose-dependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dose-dependent manner, the cells were arrested at G0/G1 phase.

结果显示：VPA对MUTZ-1细胞的生长抑制作用呈现时间和剂量依赖性；经4mmol/LVPA处理MUTZ-1细胞72小时后，细胞呈现典型的凋亡形态特征，光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体；透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加，胞装内大小不规则的染色质团块；流式细胞术结果表明，细胞凋亡率随着VPA浓度的增加而逐步增高，G0/G1期细胞比例随着VPA浓度的增加而逐渐增多，S期细胞比例逐渐减低，细胞被阻滞在G0/G1期。

Grew on the normal temperature, it was shown that the deposits of calcium antimonate being the indicator for Ca(superscript 2+) localization mainly concentrated within the vacuoles and intercellular spaces and there was also some Ca(superscript 2+) deposits in cell walls. But when Garyota urens L. was treated by the temperature of 2℃ for 48 h, the level of Ca(superscript 2+) increased in cytoplasm and plasma membrane, but decreased in vacuoles and intercellular spaces considerably. At the same time, the ultrastructure of chloroplasts suffered from chilling: the membrane of chloroplasts had been damaged, the layer of thylakoids was exiguous and unclear, the photosynthetic rate decreased evidently. And when Garyota urens L. was treated by the temperature of 2℃ for 120 h, the deposits of Ca(superscript 2+) mainly concentrated within the cytoplasm, nucleus and plasma membrane and there was also some Ca(superscript 2+) deposits in vacuoles, and the ultrastructure of some cells was simultaneously damaged severely: Chloroplasts structure, vacuole membrane and nuclear membrane had been damaged fully, the structure within the cell had become unclear, and the cell only have respiration.

研究结果表明，未经低温处理的董棕幼苗叶肉细胞，焦锑酸钙沉淀颗粒大量出现在液泡和细胞间隙中，细胞壁中也可见少量沉淀，而细胞基质中则看不到焦锑酸钙沉淀；经2℃ 48 h低温处理后，细胞基质和细胞膜上焦锑酸钙沉淀增加，而液泡和细胞间隙中的焦锑酸钙沉淀则显著减少，并且超微结构已初步显示出寒害的特徵，叶绿体外膜部分破损，类囊体片层稀疏且排列不规则，光合速率明显下降等；经2℃ 120 h低温处理后，细胞间隙内的焦锑酸钙沉淀极少，有的也紧贴在细胞外壁上，而细胞基质和细胞膜上则分布有非常多的焦锑酸钙沉淀，在核基质和液泡中也可见到少量的焦锑酸钙沉淀，并且超微结构遭到了显著破坏，叶绿体结构完全被破坏，核膜与液泡膜严重破损，内部结构模糊，细胞只表现为呼吸作用，不进行光合作用。

Results①The Rab9 and NPC1 colocalized in cytoplasm of microglia.②Rab9 expressed in lysosome,Golgi apparatus and late endosome.

结果Rab9和NPC1共定位于小胶质细胞胞浆内，Rab9表达于溶酶体、高尔基体和晚期内体。

The reservoir rock types in the region of interest are grain limestone, algal limestone, cryptite, siltstone, muddy limestone and mudstone. The thesis identified as a result of changes in the deposition causing the heterogeneity in plane, and then the geological oil reserves have been calculated in accordance with the areal heterogeneity.3、Based on the data of core analysis, bore logging, array induction imaging log and FMI, porosity and permeability model were built using regression analysis. Through testing 45 rock samples, a, b, m, n value were obtained and used to calculate single-layer oil saturation value using the Archie formula. According to the theory of laterolog and inductolog, resistivity ratio and cross plot were used to identification effective thickness. Effective thickness was identified delicately in 103 wells. Based on the over work, active porosity and oil saturation was calculated accurately.4、According to observation of 100 pieces of the thin slices, cast thin slices and 383m core in three wells, the chief diagenesis in this area were compaction, cementation, denudation and cataclasis. Cementation and denudation were universally influenced active porosity this area. The intergranular pores, residual pores and secondary pores were formed in thin carbonated reservoir of lacustrine facies.5、The influence of reservoir heterogeneity to reservoir calculation was analyzed systematically using actual data. And the conclusions were as follows:①The principal reason leading to oil distribution unequal is interior structural feature of reservoir in structural hydrocarbon reservoir.

确定了由于沉积微相的变化而导致的平面非均质性，进而在平面上按照平面非均质单元计算了石油地质储量。3、利用岩心分析、常规测井曲线与阵列感应、成像测井资料相结合，分岩性利用回归分析方法建立了储层孔隙度、渗透率模型；通过对45块岩样进行岩电试验，获取了a、b、m、n值，利用阿尔奇公式计算了各单层含油饱和度值；根据侧向电阻率和感应电阻率串并联特性，利用电阻率比值法与常规交会图法相结合重建了有效厚度图版，对全区103口井目的层段进行了有效厚度精细划分，通过以上工作，准确求取了储量计算单元内有效厚度参数、有效孔隙度参数以及含油饱和度参数。4、通过对研究区3口井383m岩心观察，100余块薄片、铸体薄片镜下鉴定得出：油泉子油田上、下油砂山组储层主要成岩作用是压实作用、胶结作用、溶蚀作用和破裂作用，其中溶蚀作用、胶结作用最普遍，这两种作用对有效孔隙度影响最大，以上成岩作用形成了原生粒间孔、粒间残余孔和次生孔隙(如粒间溶孔、粒内溶孔、铸模孔、晶间孔等)为主的薄层湖相碳酸盐岩储层。5、利用实际资料，系统分析了油藏非均质对储量计算的影响，认为：①对于构造油气藏而言，油气藏构造及断裂特征是造成油气分布不均的首要原因，储集条件相同的条件下，构造高部位有利于储存油气，油气在垂向的重力分异体现了油气藏内部构造的不均一特征。

The animal model of this method system have, the characteristics of the high fat, high leptin, high Ins, and match with the characteristic of clinical and the pathologic of simple obesity. This studies showed: The acupuncture can obviously reduce the vaule of intaking food and water of the fat rats, and lower the body weight and fat; The acupuncture can urge the diameter, area, physical volume of white fat cell contract, Brown fat tissue of fat rat group surroundings vascular containing large quantity of white fat cell, the acupuncture group was less. this showed: The acupuncture enhanced the white fat cell metabolism, and promotesed the white fat cell to convert to brown fat cell; The frequency of spontaneous electric of nerve cell of pvn of the fat rat is high, the acupuncture counld depress the spontaneous electric of nerve cell of pvn, This showed: the acupuncture can depress the appetite passing to adjust function of pvn nerve; The monoaminergic neurotransmitter of inside of the fat rat brain is disfunction, the acupuncture can rectify this disfunction, and adjust level of 5-HT, and affect appetite, and improve metabolism; The level of leptin and Ins of fat rat increased high, The level of it of acupuncture group decreased, compared with the fat group p.001, This showed: the fat rats have the defeat of leptin and Ins in the body, The acupuncture can lowering this defeat, The level of leptin and Ins of the fat rat in the brain is less than the normal group, The level of leptin of the acupuncture group is high than the fat group, This showed: the acupuncture can increase the transportation of leptin from blood to brain.

本方法所制肥胖大鼠模型具有高体重、高脂、高leptin和高Ins血症的特点，符合人类单纯性肥胖病的临床和病理特征；实验研究显示：针灸能明显减少肥胖大鼠的摄水、摄食量及降低其体重、体脂；针灸能促使白色脂肪细胞直径、面积、体积缩小，减少肥胖大鼠棕色脂肪组织血管周围白色脂肪细胞数量，显示：针灸增强了白色脂肪细胞的代谢，促进了白色脂肪细胞向棕色脂肪细胞的转化：肥胖大鼠PVN神经细胞自发放电频率增加，脑内单胺递质紊乱，针灸通过抑制PVN亢奋的自发放电，调整5-HT水平，达到影响食欲，改善代谢的作用；肥胖大鼠血中leptin及Ins水平增高，与正常组大鼠比有明显差异，提示大鼠体内存在leptin及Ins抵抗，针灸后肥胖大鼠随着体重体脂的下降，血中leptin、Ins水平降低，与肥胖组比均有显著性差异，而针灸后大鼠脑内leptin增加，与肥胖组比P.05，提示：针灸具有改善leptin、Ins抵抗及增加leptin脑内转运的作用。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。