内质体

Changes of lung ultrastructures: The results of electron microscope showed that in group 1, there were swelling mitochondria was arranged in disorder, less matrix, and hyalomere appeared, thin double-deck membrane in 50% samples: Interalveolar septum stroma was with edema in 60% samples; Polymorphonuclear leukocyte gathered in vessel or emigrated out of vessel in 50% samples; In group 2, double-deck membranes of mitochondria were integral, densely matrix showed micro-granule shape in 90% samples; Pinocytosis in epithelial cells of type Ⅰ lung strengthened, being destroyed, and pinocytosis in endothelial cells strengthened in 10% samples; lnteralveolar septum stroma was with edema, no polymorphonuclear leukocyte gathered in vessel or emigrated out of vessel or corpuscule was empty in type Ⅱ alveolar epithelium in 20% samples.

肺组织超微结构改变观察：纯氧机械通气组50%标本线粒体肿胀、排列紊乱，基质变浅，出现透明区，双层膜变薄，60%标本肺泡隔间质水肿，50%标本多形核白细胞血管内聚集或游出血管外现象；34%氧浓度机械通气组90%线粒体双层膜较完整，基质致密呈细颗粒状；10%标本可观察到Ⅰ型肺上皮细胞有吞饮增强、破坏及内皮细胞吞饮增强；20%标本肺泡隔间质水肿；未见到多形核白细胞血管内聚集或游出血管外现象及Ⅱ型肺泡上皮板层小体排空。

It turned out that many inclusions were found in the nucleolus and the cytoplasm of PK15 cell. The paracrystalline arrays was found in the inclusion in cytoplasm whose electron dense is deep; The nuclear of PK15 cell dissolved and there were margination of multiple chromatin aggregates and suspected intranuclear viral inclusion in PK15 cell.

细胞质中包涵体电子密度深，位于核膜周围，内有呈晶格状排列的病毒粒子，直径12±2nm；细胞核内染色质消散，在细胞核内发现多个电子密度深的、圆形或环状、由大量异染色质组成的包涵体，内有细微的粒状物质。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

Results 1、The CD11b-positive microglia were dyed low brown with small bacilliform cell bodies,ramified morphology,thin and long protuberance in CON and NIC group;In LPS group,the CD11b-positive microglia were dyed chocolate brown with more big roundish cell bodies,thicker and shorter processes;Most CD11b-positive microglia were dyed brown with bacilliform cell bodies,ramified morphology and few cells were dyed deep brown with more big roundish cell bodies,thicker and shorter protuberance in NIC+LPS group.

结果 1、CON组与NIC组CD11b阳性小胶质细胞胞体小，呈杆状，突起细长，染成浅棕色：LPS组小鼠脑内CD11b阳性小胶质细胞胞体增大，呈圆形，突起粗而短，染成棕褐色；NIC+LPS组少量CD11b阳性小胶质细胞胞体增大，圆形，突起变粗变短，染成深棕色，多数呈杆状，突起少、细长，染成浅棕色。

The energy-dispersive X-ray analysis showed that the endodermis with Casparian strips in primary and two-year-old leaves functioned as apoplastic barriers against Na and Cl. However, no blockage of Na and Cl in cotyledon was observed when they passed endodermis.

对相应区域的X射线微区分析表明，子叶内皮层对Na和Cl在质外体运输中不起障碍作用，而初生叶与2 a生针叶内皮层阻碍Na和Cl以质外体途径进入维管组织。

Theurinary IL-6 level positively correlated with density of glomerular matrix membrane, global sclerosis, fiber or fibrocellular crescents and interstitial fibrosis (p. 05). According to the degree of density of glomerular matrix membrane and interstitial fibrosis, urinary Col-IV level had better correlation than urinary TGF-betal and IL-6 levels.In IgAN, Col-IV showed increased expression in diseased renal tissue whereas the site of expression of TGF-betal was mainly localized within the cytoplasm of tubular epithelial cells. Interstial expressionwas also present but glomerular TGF-betal expression was found only in patients with heavy mesangial proliferation. There was a significant correlation between glomerular positivity for Col-IV and severity of histological damage. There was also a significant correlation between positivity for TGF-betal and Col-IV in the tubular epithelial and interstitial lesions. In contrast, there was no ralationship between glomerular positivity for TGF-betal and severity of histological damage.The urinary TGF-betal level paralleled tubular TGF-betal expression.

结果 ①IgAN患者尿TGF-β1、IL-6、Col-Ⅳ水平较健康人明显增高(P＜0.01)，该变化与血中的浓度无关(P＞0.05)；②尿TGF-β1水平与小管间质TGF-β1阳性表达呈正相关(P=0.000)，而与小球TGF-β1阳性表达无关(P＞0.05)，尿Col-Ⅳ水平与小球和小管间质Col-Ⅳ阳性表达均呈良好的相关性(P＜0.01)，还与小管间质TGF-β1阳性表达呈正相关(P＜0.05)：③小球Col-Ⅳ阳性表达与肾组织慢性病变密切相关(P＜0.05)，小管间质Col-Ⅳ和TGF-β1阳性表达均与肾小管间质病变呈良好的相关性(P＜0.01)，而小球TGF-β1阳性表达与肾组织损伤无关(P＞0.05)；④尿TGF-β1、Col-Ⅳ水平与肾小球基质基底膜面密度、小管间质病变呈正相关(P＜0.01)，与小球内细胞数呈负相关(P＜0.05)，该结果与其在组织中的表达一致；尿IL-6水平浙江大学硕士学位论文尿TGF一B一、IL一6和Col一IV在IgA肾病中的应用价值与基质基底膜面密度、球性硬化、纤维或细胞纤维新月体所占肾小球百分数及小管间质病变均有显著的相关性(F.05)；在轻度肾病理损伤时，尿'l'G卜pl、I卜6、Col一IV水平即升高，而尿Col一W在反映细胞外基质积聚和间质纤维化程度上比尿TGF一pl和IL一6有更好的相关性。

According to the variation in the components of intermediateacid igneous rocks coming from the lower crust, it is possible to restrain the depth of the source area and the minimum thickness of the crust, and thus to provide important information for the study of the deep process of the continental intraplate mineralizationGlobally, many world level porphyry copper deposits and metallogenic systems formed by hypabyssal hot liquid have their close relationship with the synchronous adakite in spacetime and genesis; domestically, the adakitelike rocks have been identified to be related to the metallogenesis in the main metallogenic areas in ChinaThe recognition of adakitelike rocks having no relationship with the process of subduction makes it possible to construct a metallogenic model of continental intraplate porphyry metal deposits by combining other geological evidences, and this model is totally different from the metallogenic model of porphyry copper deposits with Bsubduction setting constructed by Sillitoe (1972)The existence of adakitelike rocks may be the necessary condition but not the sufficient one for forming the largescale porphyry deposits and the hypabyssal hotliquid deposits, whose metallogenic elements mainly came from the mantleThe metallogenic potential of adakitelike rocks is achieved by the entering of the mantle material, and the metallogenic specialization of adakitelike rocks is decided by the distribution characteristics of the metallogenic elements in the upper mantleAn important reason for the adakitelike magma related to subduction being advantageous to mineralization is that there were abundant high pressure and high temperature liquid coming from the subducted platepieces and the magma of high fO2 coming from the melting of subducted platepiecesHowever, for those adakitelike rocks, whose occurrence has continental plate background but does not relate to the subduction, their metallogenic mechanism is not clear yetBased on the concept of modern mineral exploration and combined with the analysis of integrated geological information, we may better realize the practical prospecting significance of the adakitelike rocks

根据起源于下地壳的中酸性岩浆岩的成分变化，可以约束其源区深度以及地壳最小厚度，为大陆板内成矿作用的深部过程研究提供重要信息。全球范围内，许多世界级斑岩铜矿和浅成热液矿化系统与同期的埃达克质岩存在密切的时空与成因联系，在国内主要成矿区带也识别出与金属成矿作用有关的埃达克岩。与俯冲过程无关的埃达克质岩的识别，使我们有可能结合其他地质证据构建完全不同于Sillitoe(1972)B型俯冲环境的斑岩铜矿成矿模式的大陆板内斑岩型金属矿床成矿模式。对于规模巨大、矿质主要源自地幔的热液矿床的形成，埃达克岩可能是必要条件，但不是充分条件。埃达克岩的成矿潜力通过地幔物质加入而获取，埃达克岩的成矿专属性由上地幔成矿元素分布特征决定。与俯冲有关的埃达克质岩浆之所以有利于成矿，重要的原因是存在大量来自俯冲板片的高压、高温流体以及俯冲板片熔融形成高氧逸度(fO2)的熔体，但产出在大陆板内背景、与俯冲无关的埃达克岩的成矿机制还不清楚。根据现代资源勘查理念，结合综合地质信息分析，埃达克质岩具有实际找矿意义。

Therefore,tapping potencial of remaining oil to improving oil recovery,the recognition of reservoir intraformational heterogeneity in channel sandbodies is proposed and the main targets of reservoir IOR in channel sandbodies of mature oilfields are pointed out as follows:①shutting off strongly-waterflooded intervals to control ineffective circulation of injected water;②strengthening water washing in medium-waterflooded intervals in order to achieve the aimed waterflood efficiency and even to exceed it;③enhancing injection-production consistency and injector-producer ratio of non-waterflooded or weakly-waterflooded intervals for the propose of realizing effective displacement and imporving conforma nce.

根据我国东部主力油田储层绝大多数都属于河道砂体，注水开发进入高含水阶段后，层内剩余油超过层间剩余油，层内矛盾上升为主要矛盾这一特点，从挖掘层内剩余油潜力出发，提出要重新认识河流砂体储层层内非均质性，并指出高含水期河流砂体储层改善采油目标主要有：①封堵强水洗段，控制注入水无效循环；②加大中水洗段注水冲洗强度，达到目标水驱效率，并有可能超过目标效率；③提高未、弱水洗段的注采对应程度和注采井数比，达到有效驱替，提高波及程度。

The mature egg cell was an inactive cell with only a few polysomes. At the early zygote stage, a large number of ribosomal precursors were produced by the nucleolus, and many polysomes appeared in the cytoplasm, which suggests a high level of metabolism. Zygote at the dormancy stage had a small nucleolus and marked decrease in ribosomes, as shown by a few polysomes, which suggests decreased metabolism. Zygotes in the prophase of mitosis and two-celled proembryo became active again in metabolism, for a prominent nucleolus, high density of ribosomes and increased number of polysomes in the cytoplasm.

结果如下：在成熟卵细胞中多聚核糖体数量不多，且细胞代谢活性较弱；初期合子内，核仁大量合成核糖体前体物质，胞质中多聚核糖体数目众多，细胞代谢活性较强；休眠期合子的核仁变小，胞质中核糖体数量急剧减少，仅有少量多聚核糖体，细胞代谢活性较弱；合子分裂前期和二细胞原胚期，核仁显著，胞质中核糖体的密度增加，出现大量多聚核糖体，细胞代谢活性较强。

The sand bodies of the inner delta front are from under water distributary channels and under water crevasse splays, the former is good at the physical property and has a good single pass connectivity, and the latter has a high independence and can form a suit of fake- connected sand body.

内前缘主要发育水下分流河道和水下决口扇砂体，前者物性好、单向连通性好，后者单期独立性强，多期叠置后可形成砂体连片假象；外前缘砂体连片性非常强，但是砂体内部发育大量水平或近水平泥质薄层，储层的垂向非均质性较强。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。