内质体

The data shoewed that esculetin, resveratrol enhanced LPL expression up to about 1.2 ~3.5 fold compared with the control group. In addition to fibrates, however, no effect on LPL expression by curcumin was observed. We concluded that esculetin and resveratrol enhanced LPL expression on CHO-K1 cell. We hope that we can apply these drugs to mutant form LPL, furthermore apply theses drugs into clinical treatment.

利用药物处理有转染带有完整人类脂蛋白解脂酶启动子和脂蛋白解脂酶cDNA 序列(-1715 ~+1643)质体的CHO-K1细胞，从细胞培养液以及细胞溶解物中发现，除了curcumin之外所有药物都可以使得细胞培养液以及细胞溶解液内的脂蛋白解脂酶活性有1.2至3.5倍的上升，由此结果可知除了curcumin外，esculetin和白藜芦醇以及其他fibrate药物可降低三酸甘油脂血症。

MSCs were cultured in a conditional medium in subculture including: dexamethasone, vitamin C, and β-glycerophosphate. At the same time, we constructed the eukaryotic expression vector containing VEGF165gene. VEGF165 gene was transfected into MSCs by means of LipofectAMINE?2000. The stably gene expressive cells were screened with G-418 for 14 days.

在脂质体介导下将构建成功的真核表达载体pcDNA3.1- VEGF165转染到诱导培养的MSCs，G-418筛选阳性细胞克隆，将阳性克隆细胞和未转染的MSCs共同接种于β-磷酸三钙上，体外培养7天后，植入原大鼠肌肉内。

The immature stage pointed to the development of SE before vacuole membrane was ruptured.In this stage,the cell radial expanding,cell wall incrassation,sieve element plastid and P-protein producing of SE occurred.

未成熟期是指筛管液泡膜破裂之前筛管的发育阶段，在这一时期内出现了细胞的径向扩展、壁的增厚以及筛管质体和P-蛋白质的产生。

In group B, a large amount of inflammatory cell infiltration in liver tissues were noted and hepatic cells showed spotty necrosis and bleeding under the light microscope; the electron microscope showed a significant decrease of mitochondria cristae, the broadness of endoplasmic reticula, hepatic cell necrosis and karyopyknosis.

B组光镜下可见肝组织大量炎性细胞浸润，肝细胞点状坏死及出血；电镜下可见线粒体嵴明显减少，内质网扩张，出现凋亡小体及核固缩。C组上述病理变化明显减轻。

Cytolysosomes increased,cellular nucleus became twisted,rough endoplasmic reticulums swelled,and chondrosome became vacuolization.Dead intervertebral disk cells were located in ?nidi? which were made of multilayer degenerative collagen fibers.

术后8周实验组椎间盘开始出现大量死亡细胞，表现为溶酶体明显增多，细胞核扭曲，粗面内质网扩张，线粒体空泡化，死亡细胞外周的&巢&增厚呈多层排列，致密颗粒增多。

RESULTS: Neuronal degeneration and necrosis, mitochondria swelling, polyribosomes disaggregation from endoplasmic reticula, and golgiosomes dilation in the hippocampal CA1 area in the two FDP intervention groups were less severe compared with the control group.

结果：果糖干预组在神经元变性坏死、线粒体肿胀、内质网脱颗粒及高尔基体扩张等方面较FS组减轻，差异有显著性。

At the age of 19 weeks, we have observed ciliated-mucous cells, some cells presented with mucous granules in the supranuclear cytoplasm and a ciliating border with short growing shafts, while the other cells presented with large mucous granules as well as compound cilia.

小粘液颗粒细胞呈柱状，胞浆顶部含有少量球形的粘液颗粒，细胞表面有短小的微绒毛，细胞核大呈椭圆形，胞浆电子密度高，胞浆下部有粗面内质网和线粒体，核上部有高尔基体。

Preparation and exosyndrome of liposomal Gd-DTPA 1.1 Preparation small unilamellar vesicles were prepared by combining 0.92 g of distearoylphosphatidylcholinc with 23 mL of distilled watcr.

Gd-DTPA脂质体的制备和表征 1.1制备工艺精密称取一定量二硬脂酰磷脂酰胆碱乙醇胺溶于双蒸水中，置于恒温磁力搅拌器内，在69～72℃恒温条件下超声分散1小时，取一定量Gd-DTPA溶液、蒸馏水和无水乙醇与上述产物混合，获得半透明胶体状物，在室温下过夜，第二天在69～72℃恒温条件下水浴孵化1小时，同时用低流速氮气吹拂液体表面除去有机溶剂无水乙醇。

ResultsThere was no expression of Caspase-3 in group A. The expression of Caspase-3 in group C was decreased compared with that in group B（P＜0.01. In groups B and C, expansion and vacuolization of mitochondria, swollen neurons and apoptotic bodies appeared, which suggested that WBH could induce neuron injury, and the neuron injury in group C was more severe than that in group B.

结果A组大鼠海马区神经元无Caspase-3表达，B、C两组大鼠海马区神经元有Caspase-3表达，B组较C组Caspase-3表达平均灰度值减小。B、C两组大鼠海马神经元超微结构发生改变、细胞器水肿，部分线粒体空化，内质网稀疏，突触前膜、后膜结构不完整，可见自噬体结构；B组大鼠的神经元超微结构损害较C组轻。

Objective Fluorescent-labeled antisense oligomer that is hybridized to 18-bp sequences next to the start coden of human proliferating cell nucleus antigen gene was used to assess the intracellular distribution and stability of the oligonucleotides in the presence or absence of liposome.

目的 观察硫代修饰型及天然型PCNA反义寡核苷酸在脂质体介导下或直接转染人膀胱癌细胞BIU-87后在细胞内的分布及其稳定性。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。