内质

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

Positive rate was relatively high, and the foreign gene could also maintain in testicular tissue for a long time, it could still be detected in the tissue in 7 months after being injected. However. Deferent results could be gained when the detection were made with different primers on different sites of the recombinant plasmid. This could be explained by transgene lost when the foreign gene recombinated and integrated in heterogenetic cell, or transgene degradated by cell nucleases.

然而，我们也看到，在后代检测中，利用不同的引物扩增重组质粒不同部位时，检测结果存在差异，转基因阳性率不同，这种差异说明当外源基因进入到异源细胞内，在重组整合时发生了外源基因丢失的现象，并由此导致外源基因的整合率下降，也可能是受细胞内核酸酶的作用，使得外源基因遭到降解。

To find out about technologies that are truly "over the horizon" in refractive surgery, we introduce several surgical methods and new trend in the future. These include intracorneal rings, laser thermal keratoplasty, phakic in traocular lens, pico-second intrastromal laser, conductive keratoplasty,scleric expansion devices and customized LASIK.

本文整理介绍数种手术方法：包括有角膜内环扣、高温雷射角膜塑型术、眼内镜片植入术、角膜基质中光雷射、传导式角膜塑形术、巩膜扩张术、完全雷射层状角膜型术以及未来雷射角膜屈光手术之趋势。

Leucocytic differentiation antigen 40 sign-al access participates in regulation of inflammatory reaction accommodation of major cell component (vascular endothelial cell、vascular smooth muscle cell and macrophage), it is playing critical role in AS process, participates ather-osclerotic occurrence and development ,reside in atherosclerotic plaque cell thro-ugh CD40-CD40L interaction, induce activate by itself , express and secrete profit to happen immune response、inflammatory reaction、hemagglutination t-hrombogenic proteo-cytokine.

白细胞分化抗原40 (CD40)信号通路参与了动脉粥样硬化斑块内主要细胞成分(血管内皮细胞、血管平滑肌细胞以及巨噬细胞)炎症反应的调节，其在AS进程中扮演着关键性的角色，参与了动脉粥样硬化的发生和发展；存在于AS斑块内的细胞通过CD40-CD40L相互作用，导致自身活化，表达和分泌了有利于免疫应答发生、炎症反应、血凝和血栓形成的蛋白质细胞因子，如激活在AS斑块中的关键性细胞成分如黏附分子、炎症因子、基质金属蛋白酶等的产生，导致斑块的不稳定和破裂，而最终导致粥样硬化斑块病变的恶化。

BACKGROUND: Lipopexia induced by glucocorticoid is fat precipitation in marrow due to abnormal lipometabolism, or differentiation of cells resulting from hormone-affected bone marrow mesenchymal cells.

摘要背景：糖皮质激素引起的髓内脂肪蓄积是由机体脂肪代谢异常在髓内形成的脂肪沉淀，还是由于激素直接作用于骨髓间充质细胞使细胞发生了分化，以及具体生成的过程目前还没有定论。

BACKGROUND: Lipopexia induced by glucocorticoid is fat precipitation in marrow due to abnormal lipometabolism, or differentiation of cells resulting from hormone-affected bone marrow mesenchymal cells. The precise generating procedure remains unclear. OBJECTIVE: To investigate effects of various concentrations of dexamethasone on adipogenic differentiation of bone marrow mesenchymal cells.

背景：糖皮质激素引起的髓内脂肪蓄积是由机体脂肪代谢异常在髓内形成的脂肪沉淀，还是由于激素直接作用于骨髓间充质细胞使细胞发生了分化，以及具体生成的过程目前还没有定论。

Capsule ovoid, oblong, or linear-fusiform, included in marcescent perianth tube; pericarp membranous.

蒴果卵球形，长方形，或线形纺锤形，内藏于在凋存的花被筒内；果皮膜质。

Substance P distributes nonuniformly in the striatum and globus pallidus.The postnatal development of this neurotransmitter has significant difference with other neurotransmitters in the brain.

P物质非均匀性地分布在纹状体和苍白球内，纹状体和苍白球内P物质的生后发育与其他神经递质有显著的差异。

Yantai Normal College,264025,ChinaABSTRACT Most epithelial nuclei of Nuttallia olivacea are elliptic in shape and the chromatin is filiform.Most nuclei of connective tissue are round and have Feulgen positive granules.

摘 要 紫彩血蛤的鳃上皮细胞核多为椭圆形，核内染色质呈丝状；结缔组织细胞的核多为圆形，部分核内有块状Feulgen阳性颗粒。

Cystatin C may be involved in the astroglia-mediated neural plasticity events in the hippocampus following perforant path transections.

Cystatin C可能和去内嗅皮层支配海马内星型胶质细胞介导的神经可塑性事件有关。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。