内质

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

A series of profile numerical simulation are based on the datas of core flooding experiment, including the homogeneous profile positive rhythm section, the rhythm section, interlining profile, interlayer heterogeneity section, intraformational heterogeneity of reservoir section, the actual reservoir simulation. The the section of displacement mechanism of core flow method and the datas of core flooding experiments take into account, the EOR scheme of later stage is CO2 preslug followed by the N2 slug flooding, at last is the waterflooding or depletion type of drive..

为了进一步分析注CO2／N2段塞驱对扫油效率的影响，在岩芯驱替实验的基础上对N2推进CO2段塞驱进行了均质剖面、正韵律剖面、反韵律剖面、隔层剖面、层间非均质剖面、层内非均质剖面、实际油藏剖面的数值模拟研究，通过对比研究，初步认为高11油田在开发中后期提高采收率技术上可选取在适当高的压力条件下，先注前置CO2段塞驱，后续再注N2段塞顶替驱，然后再转注水或衰竭开采的驱替方式。

He type of TGF β1 expression was related with lymph node metastasis and prognosis of patients. The positivity rate of TGF β1 expression only in mesenchyma in the group on lymph node metastasis was significantly lower than that in no lymph node metastasis, but the positivity rate of TGF β1 expression only in cytoplasma was higher. The prognosis of TGF β1 expression only in mesenchyma was better, but the prognosis of TGF β1 expression only in cytoplasma or both mesenchyma and cytoplasma was poor.

GF β1的阳性表达类型与肺癌的淋巴结转移及预后有关，淋巴结转移阳性组TGF β1的单一间质阳性表达率显著低于淋巴结阴性组，而单一胞浆阳性表达率高于淋巴结阴性组；半年内死亡组的TGF β1单一间质阳性表达率低于5年以上生存组，而间质胞浆均阳性的表达率及单一胞浆阳性表达率则高于5年以上生存组(P均＜0.05)。

A statistics in world range indicates that the Sr/Eu ratios in the peraluminous granites show similar variation trend to those in pegmatitic apatites. This paper suggests that the high Sr/Eu ratio (＞300) in apatites or peraluminous granites is due to the strong partition of Eu〓 into the aqueous fluid, and this ratio can be used as an excellent geochemical indicator of fluid exsolution from magmatic melts.

对世界范围内的过铝质花岗岩统计表明，过铝质岩浆岩的全岩样品中的Sr/Eu也呈现二歧变化，由此本文提出Sr/Eu比值是指示过铝质岩浆体系中岩浆流体相出溶的有效地球化学参数，大于300的Sr/Eu比值是Eu〓强烈分配进入流体相的地球化学标志。

This pattern is characterized by the combination of pro-MMP-2 and membrane type 1 (MT1)-MMP expression, which drie pericellular generation of actie MMP-2 and local degradation of normal lier matrix. In addition there is a marked increase in expression of TIMP-1 leading to a more global inhibition of degradation of fibrillar lier collagens by interstitial collagenases (MMP-1/MMP-13). These pathways play a significant role in the progression of lier fibrosis.

其降解肝正常基质，同时抑制引起肝纤维化的纤维胶原降解，这种情况的特点是前明胶酶A合成和膜型1－MMP表达，使得小叶内细胞激活MMP-2和降解当处的正常肝基质，此外血浆1型组织基质金属蛋白酶抑制剂（TIMP-1）明显升高引起广泛的抑制间隙胶原酶(MMP-1/MMP-13)对纤维肝脏胶原的降解。

The successful transfection of perifollicular cells was quantified by elisa.

制备得到的质粒脂质体制剂能够将质粒转染进皮肤细胞并介导体内瞬时表达。

The human cartilages are composed of chondrocyte and extracellular matrix , the form of chondrocytes are hypertrophy and the quantity are less; the ECM of cartilage are compised of type Ⅱ collagen and proteoglycan. Articular cartilages are all hyaline with little fibers. Trauma and arthritis are the main cause of cartilage injury, the ommilayer injury ofcartilage can be recovered by marrow, but because of without stimulation mechanism, the new tissues are merely fibrocartilages, they can not be coincide with hyaline cartilage in menchanics; the purely damage of articular cartilage can not stimulate chondrocyte to regenerate because of without blood circulation, thus, the plerosis of articular catilage can not depend on the proliferation of local chondrocyte. Ever since, people tried their best to find a way to reconstruct articular cartilage.

中文题名人骨髓基质干细胞成软骨诱导及多孔复合材料作为细胞载体的体外实验研究副题名外文题名 Cartilage induction of human mesenchymal stem cells and experiment on compound porous materials as cells' scaffold in vitro 论文作者刘晓岚导师周江南学科专业外科学研究领域\研究方向学位级别博士学位授予单位中南大学学位授予日期2003 论文页码总数68页关键词骨组织工程软骨细胞骨髓基质干细胞壳聚糖高分子外消旋聚乳酸馆藏号BSLW /2003 /R68 /10 造成人体关节软骨损伤的原因主要为创伤和关节炎，关节软骨全层损伤可由于骨髓中间充质干细胞的高速增殖修复，但这种修复由于缺乏相应的刺激机制，只能形成纤维软骨，而不能形成符合关节生理、力学要求的透明软骨；单纯软骨部分损伤软骨组织内无血管，软骨细胞迁移迟缓，无法使损伤区域软骨细胞再生，因此，关节炎及关节创伤后的软骨修复不能依赖于软骨细胞的增殖和迁移。

The mature egg cell was an inactive cell with only a few polysomes. At the early zygote stage, a large number of ribosomal precursors were produced by the nucleolus, and many polysomes appeared in the cytoplasm, which suggests a high level of metabolism. Zygote at the dormancy stage had a small nucleolus and marked decrease in ribosomes, as shown by a few polysomes, which suggests decreased metabolism. Zygotes in the prophase of mitosis and two-celled proembryo became active again in metabolism, for a prominent nucleolus, high density of ribosomes and increased number of polysomes in the cytoplasm.

结果如下：在成熟卵细胞中多聚核糖体数量不多，且细胞代谢活性较弱；初期合子内，核仁大量合成核糖体前体物质，胞质中多聚核糖体数目众多，细胞代谢活性较强；休眠期合子的核仁变小，胞质中核糖体数量急剧减少，仅有少量多聚核糖体，细胞代谢活性较弱；合子分裂前期和二细胞原胚期，核仁显著，胞质中核糖体的密度增加，出现大量多聚核糖体，细胞代谢活性较强。

Chromatic plastic film and light conversion film has been applied in protected cultivation, and plastic films with different light quality may serve as a non-chemical approach regulating growth and thermoperiodic responses of plants. A few studies showed that light quality affected growth and yield of strawberry, but little is known about its effects on photosynthesis and chlorophyll fluorescence in strawberry leaves, and fruit quality. So it is beneficial to scientific regulating growth and fruiting for strawberry protected cultivation to study on those effects and the interactions between CO2 and N supply on photosynthesis.

而低氮影响植株生长、叶片黄化，进而影响光合作用，导致抗性减弱和产量品质下降；③CO_2供应问题：目前我国草莓栽培常在设施环境下进行，在封闭性强的设施中空气流通不畅，常导致设施内部CO_2供应不足而影响光合作用，进而影响产量和品质；④光不足与光质问题：设施栽培中，光通过玻璃和塑料棚膜后使设施内的光强与光质均发生改变，这些变化特别是光质变化对草莓生长与结果的影响程度尚无明确认识。

Expression of Bilirubin Oxidase cDNA from Myrothecium Verrucaria in E. coliThe 1. 6kb bilirubin oxidase cDNA from Myrothecium Verrucaria was excised from plasmid pGEM-T/BOX, and inserted into expression vector, pET-3a which were under the control of a T7 promoter.

用限制性内切酶将1.6kb疣疱漆斑菌胆红素氧化酶cDNA从质粒pGEM-T／BOX切下，插入到表达质粒pET-3a的T7启动子下游，构建成疣疱漆斑菌胆红素氧化酶表达质粒pET-3a／BOX。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。