内质

Staining extent and intensity were evaluated semiquantitatively and mean values for each parameter were calculated. Immunostaining with D2-40 showed positivity in 100% of skeletal myxoid chondrosarcomas, 96% of enchondromas, 95% of low-grade chondrosarcomas, 80% of chordoid meningiomas, and 75% of chordoid gliomas. Staining with S100 demonstrated diffuse, strong positivity in all (100%) chordoid gliomas, skeletal myxoid chondrosarcomas, low-grade chondrosarcomas, and enchondromas, 94% of chordomas, and 81% of extraskeletal myxoid chondrosarcomas, with focal, moderate staining in 40% of chordoid meningiomas.

我们半定量地评估了这些免疫染色的广度和强度，并且计算了它们各自的平均值。D2-40阳性表达于100%例骨的黏液样软骨肉瘤、96%例内生性软骨瘤、95%例低级别软骨肉瘤、80%例脊索样脑膜瘤和75%例脊索样胶质瘤。S100染色弥漫且强烈地表达于所有的（100%）脊索样胶质瘤、骨的黏液样软骨肉瘤、低级别软骨肉瘤和内生性软骨瘤，94%例脊索瘤，81%例骨外黏液样软骨肉瘤，还有，局灶性、中度表达于40%例黏液样脑膜瘤。

The results show that the extraction rate of hyaluronic acid is proportional to the concentration and the electric current.

结果表明，透明质酸透过膜的速度在一定范围内与料液的浓度和电流强度成正比，在pH7~9范围内透明质酸的电迁移率基本保持恒定。

Except for control group, rats in the other groups were intratracheally administered with bleomycin. Animals in pulmonary fibrosis model groups were sacrificed on day 3, 7, 14, 28 and 56. The sections of the right lung were stained by HE, Masson and sirius red. The left lung was weighed and its hydroxyproline content was assayed. The mRNAs of TGF-β1, MMP-9 and TIMP-1 in the lung homogenate were measured by semi-quantitative RT-PCR.

除N组外，其余各组采用气管内注入BLM致大鼠肺纤维化模型，分别于3、7、14、28、56d处死各组大鼠，右肺行苏木精－依红、Masson胶原及天狼猩红染色，测定左肺经脯氨酸的含量RT-PCR法半定量测定转化生长因子－1(TGF-β1)、基质金属蛋白酶－9(MMP-9)和基质金属蛋白酶组织抑制物－1(nMP-1)mRNA在肺内的表达。

Microglia is always considered to be a kind of most representative immune cell in the central nervous system.

小胶质细胞一向被认为是中枢神经系统中最有代表性的免疫细胞，生理情况下小胶质细胞占脑细胞总数的10％左右，其生理功能以吞噬清除细胞残骸为主，保证脑内内环境的稳定。

The lipid peroxidation of S. maxima cultured in the presence of 4 to 100mg Se/L analysed showed that the contents of free radicals by EPR and malondialdehyde increased, of which the latter was the products of lipid peroxidation. These resulted in the decrease of the contents of liposoluble matter and percentage of polyunsaturated fatty acids, and the increase of percentage of saturated fatty acids. Using dimethyl sulfoxide , which was oxidized by OH·to form the stable and nonradical compound, methane sulfinic acid , as a molecular probe, we found that the contents of MSA from S. maxima growing in the presence of selenium were higher than those without selenium added, i. e. selenium in vivo was able to scavenge OH. in this alga.

对4～100mg Se/L范围内培养的S.maxima进行脂质过氧化的分析结果表明，加硒条件下藻细胞EPR自由基的含量与脂质过氧化产物的含量增加，造成细胞脂溶性物质含量和不饱和脂肪酸百分含量下降，以及饱和脂肪酸的百分含量相对上升；使用DMSO作分子探针，用它与OH·的反应产物MSA来表示藻细胞OH·含量，发现加硒条件下藻细胞MSA含量低于未加硒的，即硒在藻细胞内具有清除OH·作用。

Methods Ampicillin inhibitionof E. coli Top10 pcs+ was tested at first, and then b-lactamase activity in periplasm was examined.

然后再使用抗生素抗性分析、b-内酰胺酶的酶活测定以及Western blot杂交技术，分析质粒编码的b-内酰胺酶从细胞质到细胞间质的分泌情况。

For a relatively longer intense short-pulse laser, especially when local phase matching in the laser pulse between the ponderomotive force and the wake plasma oscillation occurs, there is strong electron acceleration in the laser-excited wake field and weak acceleration inside the laser pulse.

对于脉冲相对较长的短脉冲激光，尤其是在激光脉冲内等离子体尾波振荡和有质动力发生局域的相位匹配时，在激光激发的尾波场电子有很强的能量，这时在激光脉冲内电子的有质动力加速效率很低。

Methods: BMSC were separated from bone marrow of rabbits. Three groups were divided as Group A: CPC combined with BMSC (3ml) and bFGF (100μg/ml), Groups B: CPC with BMSC (3 ml), and Groups C: CPC. Each group was implanted into the sacrospinalis muscle of rabbits after cultured for 24 h.

取兔骨髓，分离骨髓基质细胞，选取松质骨粒型自骨化磷酸钙置於板孔内， A 组复合 BMSC (3ml)和bFGF(100μg/ml)；B组仅复合BMSC(3ml)；C组仅为CPC；置於培养箱中，培养24h后植入兔骶棘肌内。

Results The mortality rate of mice in 80 mg/kg, day cyclophosphamide group was 16.7%, and T level [ at 30th day :( 1.38 ± 0.31 );45th day:( 1.15 ± 0.26 ) ] and T/LH ratio [ at 30th day:(0.163 ± 0.014); 45th day:(0.127 ± 0.023 ) ] were significantly decreased (all P＜0.05) at 30th day after induction;The concentration of MDA [at 15th day:(2.70 ± 0.41);30th day:(2.710.36);45th day:(2.67 ±0.43) ] was maintained at a high level (all P＜0.05) during the 45 days ; Number of Leydig's cells [ at 15th day:(9.65 ± 0.75 ); 30th day:( 14.05 ± 0.67 ); 45th day:(8.49 ± 072)] and layers of spermatogenetic epithelia [ at 15th day:(4.75 ± 0.82);30th day:(3.60 ± 0.49);45th day:(3.74 ± 0.43 ) ] were significantly decreased ( all P ＜ 0.01 ) and stabilized in a low level. The induced model was stable and the mortality rate was acceptable. In the 60 mg/kg, day cyelophosphamide group, the T level and T/LH ratio had no significant change (P ＞ 0.05 ), and the concentration of MDA ,number of Leydig' s cell and layers of spermatogenetic epithelia recovered at 30th day after induction. The induced model was unstable.

结果 剂量每日为80 mg/kg体重小鼠成模后死亡率为16.7%，血清T[30 d：(1.38±0.31)；45 d：(1.15±0.26)]及T/LH比值[30 d：(0.163±0.014)；45 d：(0.127±0.023)]于诱导后第30天出现显著下降(P均＜0.05)，而诱导后睾丸组织内MDA含量[15 d：(2.70±0.41)；30 d(2.71±0.36)；45 d：(2.67±0.43)]维持高水平(P均＜0.05)，生精上皮层次[15 d：(4.75±0.82)；30 d：(3.60±0.49)；45 d：(3.74±0.43)]和间质细胞[15 d：(9.65±0.75)；30 d：(14.05±0.67)；45 d：(8.49±0.72)]均显著减少(P均＜0.01)并稳定于低水平，模型稳定，死亡率适当；每日60mg/kg体重组小鼠血清T及T/LH比值于不同时段并未出现明显变化(P＞0.05)，且睾丸组织内MDA含量、生精上皮层次和间质细胞计数在30 d后有所恢复，模型不稳定；每日100 rag/ks体重组死亡率为30.0%，死亡率过高。

Then rAAV-DJ-1 was transfected into HEK-293 cells for packaging. AAV vectors encoding human DJ-1 protein were stereotactically injected into the rat SN 4 weeks prior to rotenone lesion in SN. Tyrosine hydroxylase protein was determined using immunohistochemical staining. Ethovison software allowed measurement of movement distance in 30 minutes.

并转染HEK-293细胞，进行腺相关假病毒颗粒包装，所得病毒颗粒注射到大鼠脑内感染黑质神经细胞；4周后，注射鱼藤酮于大鼠黑质相同脑区；通过免疫组织化学方法鉴定TH蛋白表达情况，采用动物行为轨迹分析软件计算大鼠30 min内运动距离。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。