内质

With DGD method, the electron microscopy observation has provided new information on the process of chromatin migration. That is: Firstly, the nucleus moved toward cell wall and a picture characteristic of synizesis stage of meiosis appeared; Secdonly, the transmigration of chromatin occured through CC, and a more extensive region constituted of nuclear skeleton left behind chromatin, which was named as"clear spaces" under light microscopy; Thirdly, at the late stage of synizesis, most of chromatin had gone into adjacent cells through CC and fused into a whole, and the nuclear skeleton left in the former cell gradually mixed with cytoskeleton in cytoplasm.

DGD—包埋去包埋电镜观察表明：百合花粉母细胞中有类似核纤层的结构存在；在染色质穿壁运动过程中，首先是细胞核向细胞壁靠拢，并可留下瞬间的运行轨迹——细胞核后方出现只有细胞骨架而少有细胞器的区域；其后是染色质开始穿壁，在穿壁染色质后方细胞核内出现无染色质仅有密集的核骨架的区域，到了染色质穿壁后期，大部分核物质都已穿至相邻细胞，并彼此融合，而残余在原穿出细胞中的核骨架己逐渐与原细胞的胞质骨架融为一体。

HRP was coupled with the fluorochrome FITC and encapsulated with liposome . pEGFP-N1 plasmid (expressing green fluorescent protein) with encapsulation in commercially available liposome was prepared. The eyes were enucleated 1, 4 and 8 weeks after zonule rupture and anterior segments comprising lenses were incubated in medium containing one of these components. Cryo-sections were made and translocation of fluorescent macromolecule from the medium into the lens and green fluorescent protein expression in the epithelium were observed by fluorescent microscopy.

制备FITC标记的HRP脂质体；制备pEGFP-N1质粒（表达绿色荧光蛋白的质粒），并用脂质体包裹；豚鼠悬韧带部分离断后1周、4周、8周取含晶状体的眼前段标本分别在含有FITC-HRP复合物、pEGFP-N1质粒的培养基中孵育，冰冻切片，荧光显微镜观察FITC-HRP复合物进入晶状体和pEGFP-N1质粒在晶状体内表达的情况，比较悬韧带离断侧与非离断侧的差异。3。

M/s. The following dimensionless relationships were obtained: Sh=2+0.073Re〓Sc〓〓〓 The desulphurization process by powder injection and blowing in RH refining of molten steel and its mechanism have been considered and analyzed. Based on the tworesistance mass transfer theory and the mass balance of sulphur in the system, a kinetic model for the process has been developed. The related parameters of the model have been reasonably determined. Modeling and calculations for the process by injecting and blowing the lime based powder flux under the assumed operating modes in a RH degasser of 300t capacity have been carried out using the model.

考虑颗粒与液流的相对速度，RH—PTB条件下熔池传质特性可由下式表征： Sh=2+0.073Re〓Sc〓考虑紊流中脉动速度引起的能量耗散，RH—PTB条件下熔池传质过程服从如下关系：〓将粉剂颗粒与钢液间的传质当作刚性的气泡与钢液间的传质处理，对RH—PTB过程有：〓考察和分析了RH喷粉脱硫过程及机理，基于双重阻力传质理论和体系内硫的质量衡算，建立了RH喷粉脱硫过程的动力学模型，确定了模型各有关参数，包括各传质系数和脱硫处理过程中钢液内参与脱硫反应的粉剂量等。

Results: ApoD was observed positively expressed in type2 astrocytes and oligodendrocytes from O2A lineage, while it was not expressed in type1 astrocytes from T1A lineage. Accordingly, we can verify the phenomenon found in the study of T1A and T2A gene different expression profile that ApoD mRNA was highly expressed in T2A and lowly expressed in T1A. Conclusion: It can be concluded that lipid metabolism related genes such as ApoD play important roles in the generation and differentiation of O2A lineage, which closely correlate with internal mechanism of lipid metabolism in the brain and myelogenesis.［Key words］astrocyte(T1A, T2A); oligodendrocyte; O2A lineage; apolipoproteinApoD

我们已采用基因芯片技术在mRNA水平研究两型星形胶质细胞的基因表达谱差异，发现ApoD在2型星形胶质细胞中差异高表达，故本文拟用激光共聚焦双重免疫荧光标记技术进一步在蛋白质水平观察ApoD在体外分化成熟的两型星形胶质细胞以及少突胶质细胞中的表达情况，以期为我们深入研究ApoD等脂代谢相关基因在脑内脂质代谢和神经髓鞘发育机制中所起的作用提供有用的实验依据，依此探讨胶质细胞发生和分化的信号机制。

This article meliorates the traditional substances, and especially selects a dozen of vitamin essence, amino acid, moisture factors and other substances extracted from natural plant tea tree oil. It can efficiently protect hair, help hair inner cell fiber to renew its vigor while dyeing, make nutrient elements to permeate into hair scales in-depth, strengthen hair metabolism, improve gradually hair inner structure, force pigment particles into hair inner rapidly and cover white hair efficiently. It can create a generation of new and healthy color.

本品改良传统基质，特别精选天然植物茶树提取物及十多种维生素精华、氨基酸和保湿锁水因子，有效保护发质，能在染发的同时，帮助发丝内细胞纤维恢复生机，使其营养成份渗透毛发鳞片深层，强化毛发的自然生理运作，逐步改善头发的内部结构，使色素粒子迅速进入发质内层，并能有效遮盖白发，为您缔造新一代的健康色彩。

Based on the detailed study of seismic tomography image in South China, and according to the geotectonic environment and shape and heat of asthenosphere upwelling, we divided the structure of lithosphereasthenosphere at the Mesozoic upper mantle of Eastern China into three types.(1)Continental platform area (North China platform and Yangtze platform): asthenosphere upwelled along paleorift; over the plume head, the granitic rocks of crust and mantle mixed and correspondingly, Au, Cu, Mo, PbZn, etc. mineralizationconcentrated regions were formed; and at the steep contact belt of asthenosphere and the thick area of lithosphere, mediatebasic complex was formed.(2)Central area of fold zone: asthenosphere was "recumbent" at suitable depth, with adequate heat and in a great extent; by heat conduction, the interior of partial crust remelted, and the crustsource granitic rocks, and correspondingly, W, Sn and rare elements mineralizationconcentrated regions were formed.

在深入研究华南地震层析成像的基础上，按照大地构造环境和软流圈上涌形状和热度，将中国东部中生代上地幔中岩石圈软流圈构造划分为3类：(1)陆台区，软流圈沿古裂陷上涌，其柱头上方形成幔壳混熔花岗质岩及相应Au、Cu、Mo、PbZn等矿集区，并于软流圈与岩石圈厚区之陡接触带形成中基性杂岩及相应Fe矿集区；(2)褶皱带中心区，软流圈在适当深度、热量充足、较大范围内&平卧&，因热传导而致使地壳内物质部分重熔，形成壳源型花岗质岩及相应的W、Sn、稀有元素矿集区；(3)褶皱带边缘区(大兴安岭南部及华南南缘)，在软流圈上涌柱上方形成幔源或幔壳混熔的花岗质岩，相应为Cu、Au、PbZn、Mo、Ag矿集区。

The reservoir rock types in the region of interest are grain limestone, algal limestone, cryptite, siltstone, muddy limestone and mudstone. The thesis identified as a result of changes in the deposition causing the heterogeneity in plane, and then the geological oil reserves have been calculated in accordance with the areal heterogeneity.3、Based on the data of core analysis, bore logging, array induction imaging log and FMI, porosity and permeability model were built using regression analysis. Through testing 45 rock samples, a, b, m, n value were obtained and used to calculate single-layer oil saturation value using the Archie formula. According to the theory of laterolog and inductolog, resistivity ratio and cross plot were used to identification effective thickness. Effective thickness was identified delicately in 103 wells. Based on the over work, active porosity and oil saturation was calculated accurately.4、According to observation of 100 pieces of the thin slices, cast thin slices and 383m core in three wells, the chief diagenesis in this area were compaction, cementation, denudation and cataclasis. Cementation and denudation were universally influenced active porosity this area. The intergranular pores, residual pores and secondary pores were formed in thin carbonated reservoir of lacustrine facies.5、The influence of reservoir heterogeneity to reservoir calculation was analyzed systematically using actual data. And the conclusions were as follows:①The principal reason leading to oil distribution unequal is interior structural feature of reservoir in structural hydrocarbon reservoir.

确定了由于沉积微相的变化而导致的平面非均质性，进而在平面上按照平面非均质单元计算了石油地质储量。3、利用岩心分析、常规测井曲线与阵列感应、成像测井资料相结合，分岩性利用回归分析方法建立了储层孔隙度、渗透率模型；通过对45块岩样进行岩电试验，获取了a、b、m、n值，利用阿尔奇公式计算了各单层含油饱和度值；根据侧向电阻率和感应电阻率串并联特性，利用电阻率比值法与常规交会图法相结合重建了有效厚度图版，对全区103口井目的层段进行了有效厚度精细划分，通过以上工作，准确求取了储量计算单元内有效厚度参数、有效孔隙度参数以及含油饱和度参数。4、通过对研究区3口井383m岩心观察，100余块薄片、铸体薄片镜下鉴定得出：油泉子油田上、下油砂山组储层主要成岩作用是压实作用、胶结作用、溶蚀作用和破裂作用，其中溶蚀作用、胶结作用最普遍，这两种作用对有效孔隙度影响最大，以上成岩作用形成了原生粒间孔、粒间残余孔和次生孔隙(如粒间溶孔、粒内溶孔、铸模孔、晶间孔等)为主的薄层湖相碳酸盐岩储层。5、利用实际资料，系统分析了油藏非均质对储量计算的影响，认为：①对于构造油气藏而言，油气藏构造及断裂特征是造成油气分布不均的首要原因，储集条件相同的条件下，构造高部位有利于储存油气，油气在垂向的重力分异体现了油气藏内部构造的不均一特征。

Using maximum flooding surface and local explosure erosion surface as the correlation framework surface in the 4-th hierarchical base-level cycles, sand-body in the 3-th hierarchical base-level cycles formed in the 4-th hierarchical base-level cycles may be correlated layer by layer. The law of the sand-body and the characters of reservoir heterogeneity formed in the 4-th hierarchical base-level cycles are discussed. The study shows:①During early period of the 4-th hierarchical base-level cycle rising, subaqueous distributary channel formed, continuously stacked vertically, and migrated in limit range laterally, and channel sand-body stretches farther longitudinally, and lacks interbeds of mud rock and siltsand in sand rock, reservoir heterogeneity is relatively weak.②During mid-period of the 4-th hierarchical base-level cycle rising, subsaqueous distributary channel migrated actively, channel divided and converged strongly to form netted sand-body and sand sheet mainly, but interbeds of mud rock and siltsand increases gradually in sand-body, reservoir heterogeneity increased.③During later period of the 4-th hierarchical base-level rising and early period of the 4-th hierarchical base-level falling, reservoir sand-body was poor, and only form thin sand-body of subsaqueous distributary channel, subsaqeuous creveas and distal mouth bar, sand-bodys are surrounded by mud and silt rock.

进一步采用以第4层次基准面旋回中相当最大湖泛面的相转换面和具有区域性暴露侵蚀作用的层序边界面为等时地层对比框架的边界，对发育于MSC2—MSC4三个第4层次基准面旋回的第3层次基准面旋回内砂体进行了逐层等时对比，探讨第4层次等时地层对比格架内储集砂体发育分布规律，及储集砂体的非均质性特点，研究发现：①第4层次基准面上升初期发育的水下分流河道，砂体呈连续叠置、侧向迁移受限，纵向上呈长距离延伸的带状产出，砂体间泥、粉砂岩夹层少，储层非均质性相对较弱；②在第4层次基准面上升中期发育的水下分流河道侧向迁移活跃，纵向延伸过程中分流汇合作用强烈，以形成网状或席状连片砂体为主，但砂体间泥、粉砂岩夹层逐渐增多，储层非均质性增强；③第4层次基准面上升晚期和下降早期，不利于储集砂体发育，仅出现少量薄的水下分流河道、水下决口扇、远—河口砂坝砂体。

The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法，研究丘脑网状核(nucleus reticularis thalami，RT)中γ-氨基丁酸(gamma-amino butyric acid ，GABA)能神经元、一氧化氮(nitrogen monoxidum，NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate，cAMP)及中缝背核(nucleus raphes dorsalis，DRN)至RT的5-羟色胺(5-hydroxytryptamine，5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP，5μg)，注射当天睡眠时间略有减少，第二日睡眠时间显著减少，觉醒时间明显增多，第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后，与生理盐水组比较，睡眠时间增加，觉醒时间减少；而RT内微量注射L-谷氨酸(glutamic acid， L-Glu， 0.2μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline，BIC，1.0μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen，BAC，1.0μg)后，产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg，0.5μg)后，与生理盐水组对比，睡眠时间略有减少，但无显著性意义；而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside，SNP，0.2μg)后可明显增加觉醒时间，缩短睡眠时间；微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine，L-NNA，2.0μg)后，引起睡眠时间增多，觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用；RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后，与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠，其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate，MOR，1.0μg)后与生理盐水组对比，睡眠时间减少而觉醒时间增加； RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride，NAL，1.0μg)后与生理盐水组比较，睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后，与生理盐水组对比，原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较，睡眠时间增多而觉醒时间减少；RT内微量注射亚甲蓝(methylene blue，MB，1.0μg)后，与NS组比较，睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 0.2μg)比较，睡眠时间减少，觉醒时间增多；大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射二甲基麦角新碱(methysergide， MS， 1.0μg )后，与对照组(DRN注射L-Glu 0.2μg，双侧RT注射NS 1.0μg)比较，睡眠时间增多，觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine，PCPA，10μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 1.0μg)比较，睡眠时间增多，觉醒时间减少；大鼠DRN内微量注射PCPA(10μg)，产生睡眠增多效应后，在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan ， 5-HTP， 1.0μg )后，与对照组(DRN注射PCPA 10μg，双侧RT注射NS 1.0μg)比较，睡眠时间减少，觉醒时间增多。

If the slope＇s rockmass is low counter-inclined or thick-interbedded with soft interlayer, its failure model is inclined to be tilting-collapsing; if the slope＇s rockmass is homogeneous and thin-bedded with hard discontinuity, its failure model is inclined to be splitting-collapsing; if the slope＇s rockmass is low sequence-inclined with densely deep tectonic joints and weathering fissures, its failure model is inclined to be sliding-collapsing.

当缓倾内层状岩质边坡的岩层较厚，岩性呈软硬互层状产出，或岩层间软弱夹层较厚时，常发生倾倒-崩落式破坏；当缓倾内层状岩质边坡的岩层较薄，且岩性较均一，或层间结构面力学性质较好时，常发生拉裂-崩落式破坏；当缓倾角层状岩质边坡岩层倾向坡外时，在陡倾构造节理和风化卸荷裂隙切割下，常发生滑移-崩落式破坏。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。