内质

Between P14 and P21,CIAPIN1 immunoreaction in the brain,heart and liverbecame much lower. However,between P21 and P28,CIAPIN1 immunoreactionin the heart,brain,liver and skeletal muscle became much lower,while with thekidney development,CIAPIN1 immunoreaction in the kidney became higher. Invarious tissues from adult mouse,CIAPIN1 immunoreaction could be seen incardiac muscle cell,brain,hepatocyte,epithelium of renal tubule,skeletal muscle,lung tissue,gastric mucosa and gland,acinus lienalis.2. Distribution of CIAPIN1 in normal fetal and adult human tissuesTo reveal the possible physiological role of CIAPIN1,we examined theexpression and distribution of CIAPIN1 in fetal and adult human tissues usingimmunohistochemistry. We found that CIAPIN1 was ubiquitously distributed infetal and adult tissues,and was localized in both the cytoplasm and the nucleus.

然而，在3个月大的成年鼠中，CIAPIN1阳性反应物在心、脑、肝和肾小管中的表达强度要低于P28小鼠；但CIAPIN1阳性反应物在成年鼠骨骼肌中较P28小鼠高。2、CIAPIN1蛋白在人5个月胚胎及成人多器官组织内的表达在人5月胚胎多器官组织中，CIAPIN1阳性反应物见于心脏、胆囊单层柱状上皮和粘膜、结肠粘膜、小肠粘膜和绒毛、肝脏、直肠腺体、胃粘膜、肾上腺束状带、甲状腺滤泡、脾索、胸腺小叶间隔、皮肤真皮层和汗腺、睾丸白膜和间质、脑组织内神经元和神经胶质、肺小支气管和肺泡、骨骼肌、肾脏皮髓质和肾小管、子宫内膜、胰腺腺泡和胰岛、卵巢、输卵管粘膜等绝大多数组织细胞。

The double membrane vesicle, which was produced by budding of the outer membrane of amyloplast, accumulated starch to form new amyloplast. The double membranes of amyloplast invaginated and the inner membrane dilated to form new amyloplast in the amyloplast.

淀粉质体增殖产生新淀粉质体有多种方式：出芽增殖、缢缩增殖、中间隔板增殖、被膜向内出泡或内陷增殖、被膜形成双层膜小泡再积累淀粉增殖，它们均是淀粉质体被膜的一种膜行为。

Changes of lung ultrastructures: The results of electron microscope showed that in group 1, there were swelling mitochondria was arranged in disorder, less matrix, and hyalomere appeared, thin double-deck membrane in 50% samples: Interalveolar septum stroma was with edema in 60% samples; Polymorphonuclear leukocyte gathered in vessel or emigrated out of vessel in 50% samples; In group 2, double-deck membranes of mitochondria were integral, densely matrix showed micro-granule shape in 90% samples; Pinocytosis in epithelial cells of type Ⅰ lung strengthened, being destroyed, and pinocytosis in endothelial cells strengthened in 10% samples; lnteralveolar septum stroma was with edema, no polymorphonuclear leukocyte gathered in vessel or emigrated out of vessel or corpuscule was empty in type Ⅱ alveolar epithelium in 20% samples.

肺组织超微结构改变观察：纯氧机械通气组50%标本线粒体肿胀、排列紊乱，基质变浅，出现透明区，双层膜变薄，60%标本肺泡隔间质水肿，50%标本多形核白细胞血管内聚集或游出血管外现象；34%氧浓度机械通气组90%线粒体双层膜较完整，基质致密呈细颗粒状；10%标本可观察到Ⅰ型肺上皮细胞有吞饮增强、破坏及内皮细胞吞饮增强；20%标本肺泡隔间质水肿；未见到多形核白细胞血管内聚集或游出血管外现象及Ⅱ型肺泡上皮板层小体排空。

The GFAP is a specific marker of astrocyte, its expression is more higher in the activity astrocyte, and finally the GFAP become the main composition of scar formations. The S -100 is a kind of acidity, dissolubilites, low molecular quantity and calcium hydronium conjugated protein, and it is mainly existed in neuroglial cell and schwann cell. It can promote the growth of axon, glial hyper-plasia and nerve divide and calcium's stability inside of cell, thus regulatin g the shape and metabolism of astrocyte . The quantity of S -100 protein and the degree of ischemia have direct proportion .

胶质纤维酸性蛋白是星形胶质细胞的特异性标记物，在活性星形胶质细胞中GFAP的表达相对更高，且最后GFAP成为胶质疤痕的主要成份。S-100蛋白是一种酸性、可溶性、低分子量的钙离子结合蛋白，主要存在于胶质细胞和雪旺细胞中，它可促进轴突生长、胶质增生、神经分化和细胞内钙的稳定，从而调节星形胶质细胞的形态和代谢。S-100蛋白与缺血的程度是成正比的。

Thirdly,The nuclear chromatin of lung carcinoma cells concentrate to periphery, the electron density of cytoplasm increased, in some cells,the heterochromatin of nucleus were blurry,and the nucleole became uniform in electron density, the formatio reticularis of nucleole disappeared.

细胞核的改变：细胞核内染色质边集，基质电子密度增高。有些细胞核内异染色质分界不清，核仁呈均一电子密度，网状结构消失。

Methods Porcine bile duct and ureter were treated using detergent sodium dodecylsulphate and 1% Triton X-100 to prepare the acellular lumens matrix. The toxic effects of different concentrations of acellular lumens matrix extract were tested by MTT to assess the proliferation of human scarfskin fibroblasts. The cytotoxicity of the target biomaterial was graded according to the national standards. The growth manner of the human intrahepatic bile duct endothelial cells seeded on the acellular lumens matrix was studied after 20 d under scanning electron microscopy.

采用十二烷基磺酸钠、Triton X-100处理封闭群小香猪输尿管和胆管，获得去细胞管腔基质支架材料； MTT法检测基质支架材料的不同浓度浸提液对细胞增殖的影响，并按医用生物材料细胞毒性分级标准进行评级；在基质支架材料上种植人肝内胆管上皮细胞，于接种后20 d通过扫描电镜观察人肝内胆管上皮细胞在支架上的生长情况。

Following PAS staining, glycogen in hepatoma carcinoma cells mainly expressed near to unilateral cytoplasm surrounding nuclei, showing strong positive expression in the positive control group. Big karyoplasmic ratio and weak expression of glycogen in cytoplasm were visible in the embryonic hepatic progenitor cell group.

PAS染色后阳性对照组肝癌细胞的糖原主要表达在靠近一侧的核周边胞质内，呈强阳性；而胚胎肝前体细胞诱导组，核浆比例大，部分细胞胞质内可见较弱的糖原表达，充满胞质。

Three of them were diagnosed as sarcoidosis. Two were pulmonary alveolar proteinosis. One with usual interstitial pneumonia, one with bronchialitis obliterous and organizing pneumonia, one with nonspecific pneumonia, one with Castleman's disease, one with pulmonary lymph angioleiomyomatosis, two with bronchioalveolar carcinoma, one as rheumatoid pulmonary fibrosis complicated with pulmonary squamatous cancer and locally metastas is in lungs, two as pulmonary lymphangitic carcinomatosis, one as lung squamatous cancer with pulmonary lymphangitic metastasis, and one with primary pulmonary paraganglioma.

结果 17例患者均获病理确诊，其中结节病3例，肺泡蛋白沉积症2例，普通型间质性肺炎1例，闭塞性细支气管炎伴机化性肺炎1例，非特异性间质性肺炎1例，Castleman病1例，淋巴管平滑肌瘤病1例，肺泡细胞癌2例，类风湿性关节炎肺间质纤维化合并肺鳞癌伴肺内多发转移1例，肺淋巴管癌2例，肺鳞癌伴肺内淋巴管转移1例，肺原发性副神经节细胞瘤1例。

Methods At first, retrovirus vectors encoding IFN-γ or IL-4 gene were constructed. They were transfected into PA317 packaging cells by lipofectamine, PA317IFN-γ and PA317IL-4 cells were obtained. C6 glioma cells were infected with replication deficiency retrovius containing IFN-γ or IL-4 gene, cell morphology、cell growth curve and cloning efficiency assay were examined. The intracranial C6 glioma animal model was established in immunocompetent Wistar rats. PA317IFN-γ and PA317IL-4 cells were stereotactically implanted into the tumor areas alone or together. The survival of tumor bearing rats were examined, tumor volumes were measured and immunohistochemical analyses of CD4〓 and CD8〓 T lymphocyte infiltration were performed.

构建和鉴定携带IL-4或IFN-γ基因的逆转录病毒载体，将其导入逆转录病毒包装细胞PA317，通过G418抗性筛选，得到携带目的基因的包装细胞并鉴定；应用携带IL-4或IFN-γ基因的复制缺陷型逆转录病毒感染C6胶质瘤细胞，观察对C6胶质瘤细胞形态、细胞生长曲线和克隆形成率的影响；建立C6胶质瘤大鼠脑内移植动物模型，将携带IL-4和IFN-γ基因的逆转录病毒包装细胞分别或联合注射到脑内荷瘤大鼠的肿瘤组织中，观察其治疗作用，并初步探讨其机制。

It show that interstitial fluid pressure changes its sig n twice within one arteriolar vasomotion period and it is therefor not important that interstitial fluid pressure is a little higher or lower than atmospheric p ressure; arteriolar vasomotion can periodically result in lymph formation and in terstitial total pressure plays an important role in this procedure; local regu l ation of microcirculation can meet metabolic need to some extent in the form of dynamic equilibrium.

利用解析法与有限元法相结合的&影响线算法&，求解了第一部分中建立的数学模型，分析了微循环系统动力学的若干重要问题，表明在微动脉自律运动周期内，间质流体压力将两次改变符号，因而某些理论中十分强调的间质流体压力是略高于或略低于大气压力的问题显得并不重要；微动脉自律运动能导致周期性的淋巴形成，间质总应力在这一过程中起着重要作用；微循环局部调节可以以动态平衡的方式在一定范围内满足代谢的需要。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。