内融合

OBJECTIVE: To compare the initial stability of lumbar spine following ALIF, PLIF or TLIF in combination with bilateral pedicle screw fixation.

目的：比较前路椎间融合术、后路椎间融合术、椎间孔入路椎间融合术加用双侧椎弓根螺钉内固定后腰椎即刻稳定性的差异。

Finally,the application of our proposed method to pix...

结果表明，相比于传统小波域内的图像融合算法，该方法得到的融合结果具有更良好的视觉质量和更优的量化指标，体现出更强的融合性能。

Cases with bone graft fusion were Resected lateral part of lesion vertebral lamina and process;2 cases were treated with contralateral interanticular process and vertebral lamina bone graft fusion; 2 cases with intravertebral fusion were treated by laminectomy and arthrectomy of one side by posterior route and USS implantating; Others cases were routine internal fixation.

行植骨融合术2 例，切除患侧椎板外半和椎间相应关节突；行对侧椎板关节突间植骨融合2例，行后路半椎板和一侧小关节切除USS 植入，椎间融合2例，余采用常规手术内固定。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Fusion with spinal internal fixator was used to treat instability of the lumbar spine. Several clinical studies have indicated that the fusion may accelerate degeneration at adjacent levels. The DYNESYS, a dynamic spinal internal fixator, is therefore designed to maintain intersegmental stability and prevent adjacent segment from degeneration.

脊椎内固定器合并融合术，主要用於治疗腰椎不稳定问题，但临床文献指出使用融合术后，加速邻近椎节之退化；因此，动态脊椎内固定器DYNESYS被开发出来解决此问题，以提供椎节间稳定度与避免邻近椎节发生加速退化情形。

Extracellular calcium is required for normal progression through the initial recognition-alignment phase of myogenesis. A net calcium influx into fusion-competent myoblasts is a requisite step in membrane fusion.

细胞与细胞之间识别和并列的初期阶段，胞外钙离子的内流是正常肌细胞生成过程所必不可少的，钙离子进入具有融合潜能的成肌细胞内，从而使细胞膜发生融合。

Objective:To compare the outcome of posterolateral fusion and interbodyfusion for lumbar spondylolisthesis.

目的：比较横突间植骨融合内固定与椎间植骨融合内固定治疗腰椎滑脱症的效果。

Treatment of spinal tuberculosis1methods between february 1998 and august 2000 , 12 patients with spinal tuberculosis were operated with anterior vertebrectomy , bone grafting and plating1 evaluation including bone fusionrate , correction of deformity and status of spinal lesion was carried out1results in an average of ten months ,spinal tuberculosis was completely cured and solid bone fusion had taken place in all 12 patients1 the time for bone fusion averaged 518 months and the average angle of correction of kyphosis was 16°1conclusions anterior plating following vertebrectomy and bone grafting offers a complete removal of lesion and decompression of the spinal canal as well as reconstruction of spinal stability1 early rehabilitation and an increased curative rate can be expected through this procedure1

目的 探讨经脊柱前路结核病灶清除的同时植骨内固定的可行性和必要性，以及对脊柱结核的治疗效果。方法1998 年2月~2000 年8 月采用脊柱前路病椎切除加植骨，并采用orion或z-plate 钢板进行椎体固定。共治疗12 例脊柱结核，观察植骨融合及矫正畸形情况和结核病灶愈合情况。结果经平均10 个月的随访，12 例患者脊柱结核均治愈，植骨与受骨区全部骨性融合，融合时间平均为518 个月，后凸矫正角度平均16°。全组病例切口均一期愈合。无手术并发症。结论本方法可经脊柱前路作较彻底的病灶清除，并较好地进行脊柱矫形和椎管减压，完成脊柱稳定性重建，有利于患者早期离床活动和脊柱结核治愈率的提高。

In contrast, deletion of the propeptide yields mature but inactive NK, the inclusion bodies of NK or denatured rNK couldn't be renatured in any condition, but with the aid of exogenously purified propeptide or Ser221Ala proNK, the inactive mature protein could be renatured by an intermolecular pathway in vitro.

此外，我们利用融合基因表达系统在大肠杆菌中高效表达了谷胱甘肽S-转移酶／NK和硫氧还蛋白／NK两种融合蛋白，经体外复性、纯化，均获得活性蛋白。以上结果显示，pro肽在体内体外，通过分子内或分子间的方式均能帮助NK分子的折叠与再折叠，作为一种分子内的分子伴侣，pro肽为NK分子正确折叠所必需。

But it can only be used when each of evidence is independent, in practical applications there are many evidences can not satisfy this condition, so how to improve this theory has become to a focus problem; More over, multi source information fusion needs data of multi platform, so how to unify data of multi platform to the same time space so as to fuse is an important factor influencing the performance of fusion.

但D-S理论只适用于证据独立的情况，实际应用中往往需要处理不独立的情况，因此对证据理论的研究和改进是摆在众多学者面前的一个重点问题；另外，多源信息融合要利用多平台信息，如何将各传感器信息统一到同一的时间空间范围内进行融合是关系到融合性能的一个重要因素。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。