内融合

The method of designing artificial knee joints through surface matching between outer face of the replaced knee joint and femoral surface of the massive allogenic bone is proposed to simplify the clinic operation.

提出了依据手术切除部位膝关节的外表面与异体骨的股骨端面的曲面匹配结果来设计人工膝关节面的方法，简化了手术方案；完成了髓内针﹑融合笼及其辅助部分的研制；对髓内针的定位位置和方向，髓内针和融合笼的联接方式进行了深入的研究，提出了相应的解决方案。

The approach first extracts mesh regions to be fused by approximating offset curves on meshes, then parameterizes the regions using harmonic mapping with holes, and the smooth mesh fusion is finally obtained by topology combination and fusion control.

首先利用网格上的近似等距线算法来抽取出待融合区域，然后对两个待融合区域进行带内孔的调和映射参数化，最后通过拓扑合并和融合控制来实现网格的光滑融合。

The effect of tat p53 on the growth of parasites was determined by counting the parasitemia changes between pre and post incubation of the fusion protein with the parasite culture for 12 h. results: tat p53 fusion protein could be transduced into p.f parasites, locating at trophozoit and late schizont stage, whereas p53 protein could not.

结果：tat p53融合蛋白可以突破红内期疟原虫各层细胞膜，进入虫体细胞内，并可根据荧光观察到融合蛋白主要分布在疟原虫滋养体和晚期裂殖体，而p53蛋白不能进入疟原虫内。

The effect of TATp53 on the growth of parasites was determined by counting the parasitemia changes between pre and postincubation of the fusion protein with the parasite culture for 12 h. RESULTS: TATp53 fusion protein could be transduced into P.f parasites, locating at trophozoit and lateschizont stage, whereas p53 protein could not.

结果：TATp53融合蛋白可以突破红内期疟原虫各层细胞膜，进入虫体细胞内，并可根据荧光观察到融合蛋白主要分布在疟原虫滋养体和晚期裂殖体，而p53蛋白不能进入疟原虫内。

The samples were smeared on slides and stained with Giemsas stain. The stained smears were observed microscopically and photographed. It showed that except typical trophozoites in binary fission. the following trophozoites of abnormal morphs were also observed. for instance abnormal trophozoites with binary fission; enlarged trophozoites with round and irregular shaped eosinophilic bodies in the cell plasma; enlarged cells containing round and irregular shaped eosinophilic bodies and flagella; enlarged cells which contain 6 or 8 nuclear-like bodies and flagella, 3 or 4 embryonic forms of daughter trophozoite in one mother trophozoite; 4 trophozoite embryonic forms with fused plasma 3 trophozoites with fused plasma;4 trophozoites with fused plasma; a pair of trophozoite with binary fission fused with another trophozoite;2 pairs of trophozoite with binary fission fused each other; a trophozoite with 1 nuclear only.

结果 除观察到典型的营二分裂法繁殖的贾第虫滋养体外，还可见到多种形态异常的虫体，包括；虫体呈非典型二分裂；滋养体胀大，胞质中有团块状和不规则形状的嗜酸性物质；在胀大的贾第虫细胞内含有团块状和不规则形状的嗜酸性物质以及鞭毛；在胀大的贾第虫细胞内含有6或8个核状物以及鞭毛；在一个母体细胞中含有3或4个子体细胞的雏形；胞质互相融合的4个滋养体的雏形；胞质互相触合的3个滋养体，胞质互相融合的4个滋养体；1对营二分裂的滋养体与另一个滋养休互相融合；2对营二分裂的滋养体互相融合；仅有1个细胞核的滋养体。

The most of the product became soluble from inclusion body by lowering induction temperature. At the same time the gene was cloned into vector pMAL-p2X, which was translated in E. coli TB1 and expressed a fusion protein. We optimized the induction time and achieved high expression. But there was no obvious product secreted in the periplasm extract. Almost all fusion protein is expressed soluble in cytoplasm.

利用融合型表达载体pTYB11-EFE融合型表达，对诱导剂IPTG的浓度和诱导温度进行了优化，通过降低诱导温度使产物由包含体变成可溶性融合型产物；利用融合型分泌表达载体pMAL-p2X构建了表达载体pMAL-p2X-EFE-7~#，对诱导时间进行了优化，获得了较高的表达量，但发现产物没有明显的分泌表达，绝大部分是以可溶形式存在于胞内。

This plas mid w as transformed into E.coli BL21 strain. The major band of about 31000 fusion prot ein could be detected by SDS-PAGE analysis and the fusion protein is more than 34% of total cellular protein.The novel hCT analogue was confirmed by Western B lotting analysis.

该基因被克隆到融合型表达载体pGEX-2T中，重组融合型表达载体nCT/pGEX-2T转化E.coliBL21，培养表达，SDS-PAGE分析可见Mr约31000的融合蛋白带，融合蛋白占细胞内总蛋白的34%。

This plas mid w as transformed into E.coli BL21 strain. The major band of about 31000 fusion prot ein could be detected by SDS-PAGE analysis and the fusion protein is more than 34% of total cellular protein.

该基因被克隆到融合型表达载体pGEX-2T中，重组融合型表达载体nCT/pGEX-2T转化E.coliBL21，培养表达，SDS-PAGE分析可见Mr约31000的融合蛋白带，融合蛋白占细胞内总蛋白的34%。

Firstly, every wavelet base is employed to fuse the multi-source image based on local energy rule, and then a set of the primary fusion images are obtained. Next, these primary fusion images are fused to obtain final fusion image within spatial domain based on local varianc's weighted average rule.

该方法首先用每个小波基对源图像进行基于局部能量的融合，得到多幅初级融合图像，然后对初级融合图像在空间域内再进行基于局部方差加权平均融合，得到最终融合图像。

Group 1 with fixation of facet joint screw had a significant increase in stiffness for extension, bilaleral bending, and colockwise rotation.

经侧后方斜向植入的单枚椎间融合器加上小关节突螺丝钉内固定融合术，在提供充分椎管减压的同时，具有损伤小、脊柱后部稳定性好、术中对马尾及神经根牵拉轻的优点，可以比较理想地取代后路双枚融合器椎间融合术。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。