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In addition, baculovirus are noninfectious to vertebrates, and their promoters have been shown to be inactive in mammalian cells. Futhermore, the expression level of some recombinant proteins is up to 1000 mg/L. Finally, recombinant proteins are soluble and antigenically, immunogenically and functionally similar to their authentic counterparts. In this article, we used the transfer vector pBlueBac of AcNPV (Autographa californica Nuclear Polyhedrosis Virus) to construct the recombinant baculovirus AcNPV-GM-CSF by in vivo recombinantion.

本文中,我们首先利用苜蓿夜蛾核型多角体病毒带β-Galactosidase 基因标记的非融合蛋白基因转移载体pBlueBac,通过细胞内同源重组的方式将hGM-CSF基因成功地插入病毒AcNPV的基因组中。hGM-CSF基因编码信号肽和完整的氨基酸序列。hGM-CSF基因在感染重组病毒的草地夜蛾培养细胞Sf9中得到表达,感染后的Sf9细胞培养液能刺激人骨髓细胞在体外形成典型的集落,表达水平可达2.7〓CFU/ml。

Conclusionthe posterior pedicle screw fixation and interbody fusion in treatment of lumbar disc herniation is one effective way.

经后路椎弓根钉内固定加椎间植骨融合是目前治疗腰椎间盘突出症有效的方法之一。

[Objective]To evaluate the effectiveness of posterior lumbar interbody fusion in the treatment of upper lumbar disc herniation.

[目的]探讨后路减压植骨融合内固定术治疗高位腰椎间盘突出症的临床疗效。

Objective: To evaluate the clinical effect of posterior lumbar interbody fusion and internal fixation for the treatment of adult lumbar spondylolisthesis.

目的:探讨腰椎后路椎间融合(Posterior lumbar interbody fusion,PLIF)及内固定治疗成人腰椎滑脱的临床疗效及体会。

Conclusion]Posterior lumbar interbody fusion is a feasible operation to upper lumbar disc herniation ,with significant therapeutic effect.

结论]后路减压植骨融合内固定治疗高位腰椎间盘突出症是一种切实可行的手术治疗方式,手术疗效明确。

Interspinous process device is a kind of non-fusion implant for posterior spinal surgery. It is classified by static system and dynamic system according to its characteristics.

腰椎棘突间内固定器是脊柱外科后路非融合置入物的一类,根据其特性及作用特点可分为静态系统和动态系统两类。

The operation of posterior discompression reduction-fixation and intertransversal fusion is a satisfying procedure.

后路减压复位内固定及植骨融合术治疗腰椎滑脱症是满意的手术方法。

The operation of posterior discompression reduction-fixation and intertransversal fusion is a satisfying procedure.

后路减压复位内固定及植骨融合术治疗腰椎滑脱症是一种满意的手术方法。

Key words] spondylolisthesis;posterior discompression;reduction-fixation;intertransversal fusion procedures;vertebral reduction rate;JOA evalustion system

关键词]腰椎滑脱症;后路减压;复位内固定;植骨融合术;椎体复位率;JOA评价系统

The authors reviewed 10 years of data obtained in patients who underwent cervical laminectomy without fusion for intradural tumor resection and who had normal spinal stability and alignment preoperatively.

作者回顾了近10年的硬膜内肿瘤切除术颈椎椎板切除后未行融合术的病人且术前病人脊椎稳定性与对位正常。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?