内融合

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

为克隆表达牛肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化，从市售北方黄牛十二指肠组织中提取总RNA，以RT-PCR方法扩增其cDNA片段，将此片段克隆于pUCmT载体中，经过特异性限制性内切酶酶切分析片段正确后，进行全序列分析。

Is the chimeric protein of human epidermal growth factor and PE40 which is recombinant protein of the translocated subunit and killing subunit of Pseudomonas exotoxin A.

EGF-PE4 0是由人表皮生长因子基因与绿脓杆菌外毒素 A的跨膜亚单位和毒力亚单位( PE4 0 )基因融合，在大肠杆菌内表达的重组毒素。

RESULTS We successfully obtained human caspase-3 gene, the genes and expression vectors of reversed caspase-3 and its fusion protein with part of the translocation domain of Pseudomonas exotoxin A. Compared with the control group, the expression of both genes caused in a similar degree the decrease of living cell numbers and massive occurrence of apoptosis.

结果成功获得了caspase-3基因，构建了重构型caspase-3基因和重构型caspase-3与绿脓杆菌外毒素转膜结构域部分肽段的融合蛋白基因及其表达载体，与对照组相比，在转染后1～4 d内，两种基因的表达均导致HeLa细胞存活数减少，大量细胞发生凋亡，且二者活性相当。

［Objective］To discuss the methods of fenestration decompression,PLIF and internal fixation for degenerative lumbar instability.

：［目的］探讨开窗减压、椎间植骨融合内固定治疗老年退变性腰椎不稳。

After the nucleus of the sperm has united with the nucleus of the egg, the result of their union, the zygote, begins a process of internal division.

受精以后数小时内，也就是精子的细胞核与卵子的细胞核融合以后，所形成的这个受精卵开始内部的分裂过程。

One great biological puzzle is why membrances frequently fuse intracellularly, but rarely intercellularly.

一个生物学的大迷团是为什么膜层经常在细胞内而很少在细胞间融合。

But the interest protein was still expressed intracellularly. In this study, we have constructed expression vectors. Bovine IL2 has been expressed in Pichia pastoris, but in a fused protein form existing intracellularly. The effection of signal peptide and its procession on foreign protein secretion have been studied.

总之，本研究通过不同构建策略的表达载体的构建，实现了目的蛋白在真核体系中的表达，但以融合蛋白的形式存在于胞内，通过不同信号肽和信号肽加工位点的构建，探讨了影响目的蛋白有效分泌的原因。

Objective To explore the effects of intraventricular injection of EphB2-Fc on activation of inherent neural stem cells after cerebral cortex infarction.

目的探讨脑室内注入EphB2-Fc融合蛋白对大脑皮层梗死后内源性神经干细胞活化的影响。

People in rural areas are evenless likely to mix than those in large cities such as Johannesburg.

那些生活在农村地区的人也不比生活在像约翰内斯堡那样的大城市里的人更喜欢融合。

In comparison, the Core CPU can perform 4 DP FLOPS/cycle and then some: a 128 bit multiply, a 128 bit add, a 128 bit load, a 128 bit store and then perhaps an ALU or fused compare and jump instruction in the last dispatch port.

Core可以在一个周期内完成4个双精度浮点操作以及其他一些指令，具体地说就是：一个128bit浮点乘法，一个128bit浮点加法，一个128bit的内存读取，一个128bit的内存写入，或许还有一个ALU指令或者一个Macro-ops融合之后的比较条件跳转指令。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。