内融合

METHODS: Models of ALIF, PLIF and TLIF were simulated. After examination as normal group, the samples were randomly divided into three groups (n=5). Besides anterior, posterior and transforaminal lumbar interbody fusion include anterior, bilateral pedicle screw fixation was performed.

模拟临床手术建立前路椎间融合术、后路椎间融合术、椎间孔入路椎间融合术3种椎间融合术模型，先将所有标本作为正常对照组进行测试，然后将标本随机分为3组，每组5个标本，制成单纯椎间融合组，分别采用3种椎间融合术测试后，在此基础上再附加双侧椎弓根螺钉内固定进行测试。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在囊胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

All iliac hone graft fused well and a great deal of bony callus was found the vertebral body surface. Lucency area was in seen surrounding the hone graft, titanium and AC-AFF on the X-ray films, and there wan new bone formation in the interface of internal union and bone. The central region of titanium and AC-AFF became vague and new bone formation was found in the intracavitary aim of implants by CT scan. And port of the new hone transited the parietal reticulation and connected with normal ones at titanium and AC-AFF.

髂骨植骨组融合情况良好，融合界面上有较多的骨痂生成。X线摄片见植骨块、钛网、AC-AFF中空区域模糊且有骨组织生长，内植物周围无透光带存在，植骨界面上有骨桥形成；CT扫描在钛网及AC-AFF组可见内植物腔内形成的新骨通过其四壁的网眼结构与周围骨质相接，说明所有标本均达到骨性融合，但其中以AC-AFF组形成的新骨数量为多，且较为成熟。

METHODS: A total of 200 volunteers who need to receive spinal fusion in the Affiliated Hospital of Qingdao University Medical College were selected, including 102 cases received anterior cervical subcorpectomy combined with iliac bone implantation fusion following decompression, and 98 cases received posterior laminectomy for decompression combined with intertransverse process fusion. Meantime, 223 normal adults were served as the control group.

随机选择青岛大学医学院附属医院住院患者中需行脊柱融合的自愿受试者200例，其中行颈椎前路椎体次全切除减压自体髂骨植骨融合钛板内固定者102例，腰椎后路椎板切除减压横突间植骨融合内固定者98例，以223名健康正常人作对照。

Within 6 months, all 40 patients with cervical plates had fusions, and the cervical lordotic alignment was increased (2.51±6.75), and without plates and bolt loose or break. Of the 30 patients without plates the cervical lordotic alignment were decreased (1.33±3.43)(P.01), 2 patients had not fusions within 6 months (the fusion rate was 93.3%).

植骨融合率和颈椎生理曲度：植骨加钛板内固定组术后6个月内植骨融合率100%，颈椎生理曲度维持良好，较术前增加(2.51±6.75)°，无钢板和螺钉松动或断裂；单纯植骨组术后6个月内植骨融合率93.3%，颈椎生理曲度较术前减少(1.33±343)°，两组比较差异有显著比(P.01)， 2例术后3个月假关节形成。

［Objective］ To analyze the outcome of internal fixation for occipitalization with atlantoaxial joint dislocation by posterior decompression and occipitocervical fusion ［Method］ From December 2005 to June 2007,8 patients with occipitalization and atlantoaxial joint dislocation received removal of the posterior arcus of atlas and the enlargement of the posterior edge of the foramen magnum after skull traction performing for an average of 135 daysAll patients were operated on by posterior craniocervical fusion using cervifix internal fixation system and autologous ilium graftsThe clinical efficacy after operation was analyzed by Japanese Orthopaedic Associationneural function score ［Result］ All the patients were followed up from 6 months to 2 years, average of 15 monthsNo complication was foundAtlantodental interval was 5~9 mm before and 4~6 mm after skull tractionAtlantoaxial joint dislocation didn't completely reducedThe neurological defects were improved to some extents according to the JOA scoreImageology showed all patients had full decompression and bony fusionThe loosening or broken internal fixation was not found ［Conclusion］ Posterior decompression and fusion is a feasible method for the treatment of occipitalization with atlantoaxial joint dislocation,and the clinical effect is satisfactory

分析后路减压枕颈融合内固定术治疗合并寰枢关节脱位的寰椎枕骨化临床疗效。［方法］2005年12月至2007年6月间，对8例合并寰枢关节脱位的寰椎枕骨化患者在行颅骨牵引治疗一段时间（12~16 d，平均135 d）后采用枕骨大孔后缘扩大，寰椎后弓切除减压取自体髂骨枕颈融合Cervifix系统内固定术，手术后采用日本骨科学会神经功能评分分析临床疗效。［结果］8例患者随访6个月~2年，平均为15个月。8例患者无一例出现术后并发症，术前寰齿前间隙为5~9 mm，经颅骨牵引后为5~7 mm，寰枢关节脱位未能完全复位。手术前后JOA评分示神经症状均有不同程度恢复，影像学检查示枕颈区减压充分植骨区获得骨性融合，无一例出现内固定松动或断裂。［结论］合并寰枢关节脱位的寰椎枕骨化患者术前仔细评估影像学改变，采用颅骨牵引一段时间后行后路减压枕颈融合内固定术的治疗方案是合理可行的，且临床效果满意。

There was no pseudarthrosis or graft block fracture.

碎屑移植物能够容易的和骨形态蛋白混合而加速融合。28就象这个系列中显示的一样，移植物很快改建在3月内融合。

RESULTS AND CONCLUSLON: A total of 2 of 6 autogenous bone grafting cases suffered from graft bone dislocation, 1 suffered from pseudoarthrosis formation, and 1 suffered from donated ilium. Six of 21 autogenous bone graft with cervical plate-screw fixation suffered from loss of intervertebral height, and 3 suffered from pain of donated ilium.

结果与结论：6例单纯植骨患者中2例发生植骨块脱出，1例发生融合节段假关节形成，1例发生髂骨供区疼痛；21例椎体间自体髂骨植骨内固定患者中6例发生椎间高度丢失，3例发生髂骨供区疼痛；65例椎体间钛网植骨融合内固定患者中11例发生内植物下沉，椎间高度丢失，1例发生内固定断裂。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。