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CaN Aβ gene silencing can reduce myocardial hypertrophy in cultured cells, si1280 (21bp) of CaN Aβ gene is the most effective target site for siRNA. The method of intrapericardial injection of plasmid, microbubbles and erzymes can improve transfection efficiency of non-viral plasmid with satisfying targeted transfection. But the scope of transfected myocytes is still limited. CaN Aβ shRNA expressing plasmid transfection in vivo by pericardial injection results in decreased CaN Aβ protein expression of small part of myocytes, and CaN Aβ mRNA only shows decreased trend. The dosage of non-viral vector and the parameters of ultrasound energy should be optimized in further study.

结论RNAi技术抑制CaN Aβ基因表达可有效减轻Ald诱导的心肌细胞肥大程度;CaN Aβ基因中si1280(21bp)片段为实现RNAi的更有效片段;微泡+酶类心包腔内注射超声导入的方法可有效改善非病毒载体在体心肌的转染效率,同时具有一定的靶向性,但总的转染范围仍然有限;采用这一方法进一步进行&一肾一夹&心肌肥大模型大鼠在体心肌细胞的CaNAβ的RNAi干预,发现心肌肥大大鼠心外膜下局部心肌细胞CaN Aβ蛋白水平降低,CaN AβmRNA水平虽有下降趋势,但无统计学差异,提示质粒的用量及超声导入的参数有待进一步研究使其优化。

Immunofluorescence staining shows that, the CaN Aβ protein expression of intrapericardial injection group decreased in some myocytes under epicardium, while other groups show no decreased fluorescence.

三在体心肌CaN AβsiRNA表达质粒转染后,转染各组与心肌肥大模型组CaN AβmRNA水平比较无差异(P>0.05),但免疫荧光结果显示心包腔内途径CaN Aβ干扰组较心肌肥大模型组心外膜下部分心肌荧光减弱。

We conducted a trial to clarify the therapeutic role of intrapleural streptokinase.

我们进行了一项试验,以阐明胸膜腔内应用链激酶的治疗作用。

BACKGROUND Intrapleural fibrinolytic agents are used in the drainage of infected pleural-fluid collections.

背景在感染性胸腔积液引流中,应用了胸膜腔内溶纤维蛋白制剂。

Objective:To investigate the clinic effect of intrapleural urokinase in treatment of pleural thickening and loculated effusions by tuberculous pleural effusion.

目的 :探讨胸膜腔内注入尿激酶(urokinase,UK)对结核性渗出性胸膜炎所致胸膜肥厚和粘连包裹性积液的影响。

The patient received treatment with ceftazidime and intrapleural instillation of urokinase to promote drainage of empyema.

我们使用静体注射抗生素合并肋膜腔内投与尿激酶以加强肋膜积液引流来治疗病婴。

Intrapleural pressure is usually negative pressure.

胸膜腔内压通常为负压。

It may be an effective intravesical treatment agent for superficial bladder cancer.

大蒜素可能是一种有效的表浅膀胱癌腔内治疗药物。

It may be an effective intravesical treatment agent for superficial bladder canˉcer.

其可能作为一种表浅膀胱癌的腔内治疗药物而应用于临床。

Objective To summarize the experience of intraperitoneal repair of the giant abdominal incisional hernia using Composix kugel patch.

目的:总结Composix kugel自膨式补片腹膜腔内修补腹壁巨大切口疝的经验。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。