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We used Aeromonas hydrophila which we separated from the diseased Paralichthys olivaceus to made a inactivated whole cell bacterin .We injected this vaccine in the mice and the Paralichthys olivaceus .Both the mice and the Paralichthys olivaceus gained a good protection of 80% and 70%.The fish with dipping immersion gained a good protection of 85%,and the incidence of disease was 6%.We detected the antibody level in the antiserum by agglutination reaction and indirect ELISA which seemed to be immunogenic crucially.We anatomized the mice then we found that all the purtenance of the immunzied mice are healty.All of this proved that the vaccine is effective.

本实验将所分离到的嗜水气单胞菌用终浓度为0.2%~0.5%的甲醛对菌体进行灭活,制备了灭活疫苗,并优化灭活疫苗的灭活条件;将制备的灭活疫苗分别注射小鼠和牙鲆,均取得了较为理想的效果,其中小鼠的免疫保护率达到80%,牙鲆的免疫保护率达到70%;采用渗透压差法大规模浸泡免疫牙鲆也取得了很好的效果,浸泡免疫过的牙鲆发病率仅为6%,攻毒后免疫保护率为85%;通过应用间接ELISA方法检测受免动物的抗血清,并将牙鲆的抗血清注射新西兰白兔,从而获得兔抗鱼血清,更便于检测,结果都表明动物体内产生了较高的抗体水平;剖检攻毒后的动物,发现免疫过的动物内脏健康无异样,表明该疫苗安全有效,从而为防治牙鲆腹水症开辟了一条新途径,也为进一步制备其基因工程疫苗奠定了基础。

Rosiglitazone can decrease the expression of RBP4mRNA in 3T3-L1adipocyte that used dexamethasone induced into insulin resistance model, and can improve the expression of GLUT4mRNA. 3. Rosiglitazone can increase the glucose intake in 3T3-L1 adipocyte. Chapter 2 Superficial Observed the Expression of Rebinding Protein 4 in OLETF rats OBJECTIVE Compare the level of the expression RBP4 between OLETF rats and LETO rats in adipose tissue; observe the function that rosiglitazone interfere in the expression of RBP4; observe the relationship between RBP4 and GLUT4, then approach the possible mechanism that RBP4 induce to insulin resistance.

第二章罗格列酮对OLETF鼠RBP4的影响,以及RBP4在OLETF鼠内脏脂肪表达的初步观察比较自发性2型糖尿病OLETF鼠与同家系LETO鼠脂肪组织视黄醇结合蛋白(RBP4)水平;观察罗格列酮对RBP4的干预作用;观察脂肪组织RBP4与GLUT4水平的相关性,从而探讨RBP4导致胰岛素抵抗的可能机制。

Autologous haematopoietic stem cell transplantation has been used to treat SSc,and phase Ⅰ-Ⅱ studies have shown that it can result in sustained improvement of skinthickening and stabilisation of organ function,delay progression and prevent recidivation.

自体造血干细胞移植术治疗重症SSc患者,Ⅰ和Ⅱ期临床研究证明其能够有效改善皮肤硬化、增厚,且能够稳定内脏器官的功能,延缓疾病的进展,防止复发,疗效较稳定。

Objective To establish an artificial somatic-autonomic reflex arc in rats and observe the following distributive changes of neural fibers in the bladder.

目的 观察体神经-内脏神经人工反射弧建立后,大鼠膀胱肌间神经丛分布的改变以及神经肌肉接头处的变化。

Spina bifida ; neurogenic bladder ; artificial reflex arc ; magnetic resonance imaging ; spinal nerve root

脊柱裂;神经性膀胱;人工体神经-内脏神经反射弧;磁共振成像;脊神经根

METHODS: Neonatal rats from the model and electroacupuncture groups were used to establish rat models of chronic visceral hypersensitivity by the saccule stimulation method.

模型组和电针组乳鼠采用球囊刺激法制备内脏高敏感性大鼠模型。

And how does the visceral malaise affect the behavioral response to the intake of sapid solution?

内脏不适是如何影响正常大鼠对各种不同的味觉物质摄食的?

Over the years, these birds have learned to scavenge fish guts and undersized fish tossed back by fishing boats.

这些年来,这种鸟类学会了以捕鱼船丢弃的鱼内脏和小鱼为食。

Secondly,we successfully isolated microsatellite sequence of W.pigra using two methods and develop its SSR markers application in W.pigra.The detail contents and results are listed below: 1.Growth aquaculture habits and quality assessment research(1) Using the methods of paraffin-embedded myocardial specimens and photomicrography,we dissected and analyzed the visceral organ of W.pigra,including of cavity,swallow,esophagus,crop,bowel,rectum,parasomal sac stomach,testicle,seminal vesicle,vasa deferentia duct,ejaculatory ball,prostate gland and skin.

本研究首先从蚂蟥内脏结构、皮肤表皮细胞类型的分布等形态学研究开始,进一步研究了蚂蟥的生理、生长、繁殖习性和药材中农残、重金属的积累,并对不同炮制品、不同种质的蚂蟥的内在质量进行系统的评价。

The serous membrane that lines the walls of the abdominal cavity and folds inward to enclose the viscera.

腹膜腹腔壁周围的浆膜,向内折迭以保护内脏

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Lao Qiu is the Chairman of China Qiuyang Translation Group and the head master of the Confucius School. He has committed himself to the research and promotion of the classics of China.

老秋先生为中国秋阳翻译集团的董事长和孔子商学院的院长,致力于国学的研究和推广。