内胞浆

Results The experiment of immunohistochemical double-staining showed there were cardinal red granules in violet cytoplasm of VEGF-C positive TAM, and VEGF-C was weakly positive in intratumoral tissue, and strongly positive in peritumoral tissue.

结果 免疫组织化学双重染色显示：VEGF-C阳性表达的TAM中可见在紫黑色胞浆内有深红色颗粒存在，且VEGF-C在肿瘤内呈弱阳性表达，而在肿瘤周围间质的TAM中表达呈强阳性。

The re was no significant difference between the contents of SP and VIP of lung tissue in experiment group and those in control group.4. Ultrastructurally, after infected with IBV, lots of grume were observed on the surface of trachea tissue and the cilia of the trachea were massed.

免疫电镜观察结果显示，大多数肾小管上皮细胞胞浆内均见有SP及VIP阳性颗粒，在上皮细胞间还可见有DNES细胞；肺脏中异嗜性白细胞颗粒也常呈SP及VIP阳性反应，肺脏内也可见DNES细胞。

Results After oral administration of ST pinocytosis vesicles began to increase in endothelial cells of blood vessel in 1 hour,apocrine of ependymal cells began to increase and myelin sheath degeneration appeared in 2 hours,extensive myelin sheath degeneration and mitochondrion degeneration and incisure and vacuoles in nucleus of neuron were found in 4 and 8 hours.

结果 ST灌胃后1 h血管内皮细胞吞饮囊泡开始增多，2 h组室管膜细胞的顶浆分泌增多，部分神经髓鞘变性；4 h和8 h组神经纤维广泛变性，神经元线粒体变性、核切迹和空泡出现；16 h组神经元胞浆内线粒体变性更加明显，并出现脂褐素。

Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有按摩明显的阻抑作用，加药48h后增殖的抑制率可达43%以上；(2)ATP处理的U937细胞周期发生改变，G1期细胞数按摩明显增多，ATP作用24h时G1期前出现亚二倍体峰——凋亡峰，凋亡细胞数为3.4%，作用48h时凋亡细胞数增多为22.7%；(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜，逐渐向核膜外移动，进入胞浆内再移向质膜内外面，紧贴质膜外面再逐步脱离细胞体，进入细胞基质中，成为游离的凋亡小体。

Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used. Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有明显的阻抑作用，加药48h后增殖的抑制率可达43%以上；(2)ATP处理的U937细胞周期发生改变，G1期细胞数明显增多，ATP作用24h时G1期前出现亚二倍体峰——凋亡峰，凋亡细胞数为3.4%，作用48h时凋亡细胞数增多为22.7%；(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜，逐渐向核膜外移动，进入胞浆内再移向质膜内外面，紧贴质膜外面再逐步脱离细胞体，进入细胞基质中，成为游离的凋亡小体。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果：1合成的CS-PAA磁性聚合物微球呈球形，大小均一，TEM测定其干态下粒径为80nm左右，磁化曲线证实具有超顺磁性，具有一定的PH敏感性，固载表柔比星后，水溶液性状稳定，无沉淀物，DLS测定直径约200nm左右，测定载药率为15％，较文献报道高，包封率为20％。2肉眼观察试验组大鼠膀胱表面呈褐色，可见大量的Fe粒子聚集，普鲁士兰染色法显示，试验组大鼠膀胱壁内有大量的Fe粒子，分布至膀胱壁全层，与对照组大鼠相比，试验组大鼠的肝、脾内的Fe粒子聚集量明显降低；HPLC测定结果与Fe染色相同；高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高，其他组大鼠血生化指标没有明显变化。3MTT法发现，高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组，FCM发现试验药物组可引起明显的肿瘤细胞凋亡，试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C，试验组细胞Bcl-2蛋白减少，Bax蛋白变化不明显，Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P＜0.01)，其中高剂量组的抑制率最高。

The distribution of fibronectin in human oocyte and 2-cell to morulae embryos was also investigated, and the possible roles of fibronectin in fertilization, the development of early embryo and implantation were also discussed.

应用免疫组织化学方法和图像分析技术研究整合素β_3、FN和ER、PR在输卵管粘膜内的表达和变化；采用间接免疫荧光技术对FN在人卵母细胞和早期胚胎内的表达分布进行观察；光镜观察输卵管粘膜上皮细胞的形态学变化。结果：1。整合素β_3在人正常输卵管粘膜上皮纤毛细胞的胞浆内表达。

Positive staining of oxytocin and vasopressin were in the cytosol of the neurons and the former gave less intensity,many positive varicosities for vasopressin were also noticed in neurite.

催产素的免疫阳性产物主要在细胞核周围的胞浆即核周质内，而加压素的免疫阳性物质除了在核周质内有很强的反应外，在突起内也可见很强的免疫反应。

Stimulated astrocytes with Acetyl-β-Methylchline Chloride , Caffeine , Thapsigargin and carbonyl cyanide p- trifluomethoxphenylhydrazone by different types of combination, and observed their effects on 〓.

发现星型胶质细胞内的钙库包括：〓敏感和non—〓敏感的两种内质网钙库，可分别被MCh和CAF激活；线粒体钙库参与胞浆内Ca2+缓冲系统。

Some cell dropped into the cavity and became free. Thrombosis or part organization could be seen. The internal elastic layer became thin, disappear or broken. In internal and middle layer existed fibroblasts, fibrocytes and collagen. Some of the wall indicated hyaline change, soomth muscle cell decreased greatly. The massive inflammatory cells invaded the middle and external layer. There were many foam cells in the capsule tissue. Cytoplasm was filled with fatty tissue and cholesterol. some cavities were full of thrombosis. Some thrombosis was fibrosis, the bottom was organization. The surface of the thrombosis existed red blood cell and librae.(2)Elatic fibrila staining: the internal elastic menbrane almost completely disappeared, the intact internal elastic menbran could be seen in the new small vessels.

动脉瘤管壁厚薄明显不均，全层或局部区域显著变薄向外膨出，内皮细胞空泡变性或坏死脱落，部分内皮细胞剥离并突入管腔成游离状，可见血栓形成及部分血栓机化；内弹力板变薄、消失或突然中断；在内膜及中膜部位主要为纤维母细胞、纤维细胞和大片胶原；部分动脉瘤壁呈均质状玻璃样变，平滑肌细胞明显减少；中膜和外膜可见大量的炎性细胞浸润；瘤壁组织有纤维母细胞、纤维细胞、大片胶原成分及较多泡沫细胞，胞浆内充满脂类物质及胆固醇结晶；部分动脉瘤腔内充满血栓，有的血拴已经纤维化，血栓基部机化，血栓表面有红细胞和纤维素。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。