内胞浆

OPN Immuno-electron microscopy show the gold markers in thecraniopharyngioma cell of the ameloblast type be located on the endoplasmicreticulum that the swollen oncocyte afterbirth strach, from endoplasmic reticulumcreation behind secrete the outside of the afterbirth; But didnt see the gold markers inthe craniopharyngioma cell of the squamous type.

OPN免疫电镜显示成釉细胞型颅咽管瘤中金标OPN颗粒的产生位于肿瘤细胞胞浆的内质网上，由内质网产生后分泌到胞外；而在鳞状细胞型颅咽管瘤细胞中未见金标OPN颗粒，少量可见颗粒仅见于肿瘤间质中的血管内皮细胞上。

2 The result of electron microscope cytochemistry stain: The positive production of ACPase was present black lead phosphate deposit with high electron-dense under light microscope, located in cytoplasm or apophysis mainly. Lysosome was located in cytoplasm in the earlier of differentiation, then was present in apophysis with it grew.

4.2 电镜酶细胞化学染色结果：电镜下ACPase阳性产物为电子致密度高的黑色磷酸铅沉淀，主要分布于神经元的胞浆中或突起内，神经元分化的初期，溶酶体集中分布于胞质内，随突起的长出，可见在突起内出现。

The result showed that there was no expression in the normal control group and an apparent expression of ERK1/2 and Elk in the asthma group. A rather dense expressing of ERK1/2 was at bronchiole and mucous membrane, sub mucous membrane, smooth muscle, cytoplasm and nuclei of the out layer of the smooth muscle cell and an expression of positive fiber at submucous membrane.

结果发现正常肺内没有发现ERK1/2和Elk的表达，而哮喘时肺内有明显的ERK1/2和Elk表达，ERK1/2较密集表达在小支气管和细支气管的粘膜层、粘膜下层、平滑肌层和平滑肌外层细胞的胞浆和胞核中，也可见粘膜下层有阳性纤维表达。

In the normal group, electron microscopy showed normal chondrocytes were oval, and cells and cell membrane were intact. In cytoplasm, there were abundant rough endoplasmic reticulum, dictyosome, and mitochondria with intact nucleus, and even chromatin. In the model group, chondrocytes exhibited obvious pyknosis and their shape was irregular. The areolae around cells disappeared; cell organs in cytoplasm coagulated to flaps which showed electron dense and was uneasy to observe; the shape of nucleus showed irregularity, and caryotin gathered densely.

电镜下正常对照组软骨细胞呈椭圆形，细胞及胞膜完整，包质内可见丰富的粗面内质网、高尔基体、线粒体，细胞核完整，染色质分布均匀；模型对照组软骨细胞明显固缩且外形不规则，细胞周晕消失，胞浆内细胞器凝成高电子密度的片状物不易分辨，细胞核不规则，染色质浓聚，散裂于核中。

OVCs were isolated by density ladder centrifugation in the 4th week, and then OVC's morphology was observed under transmission electromicroscrope and immunocytochemistry were performed to detect the expression of ICAM-1. Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast.

结果 透射电镜下观察到OVCs超微结构的改变为核大，核/浆比大，核仁小，胞浆内细胞器少，可见少量内质网和小线粒体，整个细胞呈幼稚、未分化状态；ICAM-1免疫细胞化学检测阳性；损伤肝组织HE染色和ICAM-1免疫组化检测显示，肝损伤初期，Hering管周围OVCs增生并且ICAM-1阳性表达，随着肝损伤的加重，OVCs继续增生，并且，OVCs沿增生的肝纤维向肝小叶迁移，同时，ICAM-1阳性表达也继续增多，并逐渐向肝小叶弥散分布；透射电镜下观察到，肝损伤初期，OVCs在Hering管周围增生，肝纤维随之增生，增生的OVCs与肝纤维黏附生长。

OVCs were isolated by density ladder centrifugation in the 4th week, and then OVC's morphology was observed under transmission electromicroscrope and immunocytochemistry were performed to detect the expression of ICAM-1. Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast. In initial stage of damaged liver, OVCs and the expression of ICAM-1 mostly distributed around Hering duct, and then gradually increased and expanded toward hepatic lobule, shown by staining paraffin sections with HE, immunochemistry and transmission electromicroscrope. Immunocytochemistry indicated that ICAM-1 expression on OVCs was positive.

结果 透射电镜下观察到OVCs超微结构的改变为核大，核/浆比大，核仁小，胞浆内细胞器少，可见少量内质网和小线粒体，整个细胞呈幼稚、未分化状态；ICAM-1免疫细胞化学检测阳性；损伤肝组织HE染色和ICAM-1免疫组化检测显示，肝损伤初期，Hering管周围OVCs增生并且ICAM-1阳性表达，随着肝损伤的加重，OVCs继续增生，并且，OVCs沿增生的肝纤维向肝小叶迁移，同时，ICAM-1阳性表达也继续增多，并逐渐向肝小叶弥散分布；透射电镜下观察到，肝损伤初期，OVCs在Hering管周围增生，肝纤维随之增生，增生的OVCs与肝纤维黏附生长。

Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast. In initial stage of damaged liver, OVCs and the expression of ICAM-1 mostly distributed around Hering duct, and then gradually increased and expanded toward hepatic lobule, shown by staining paraffin sections with HE, immunochemistry and transmission electromicroscrope. Immunocytochemistry indicated that ICAM-1 expression on OVCs was positive.

结果 透射电镜下观察到OVCs超微结构的改变为核大，核/浆比大，核仁小，胞浆内细胞器少，可见少量内质网和小线粒体，整个细胞呈幼稚、未分化状态；ICAM-1免疫细胞化学检测阳性；损伤肝组织HE染色和ICAM-1免疫组化检测显示，肝损伤初期，Hering管周围OVCs增生并且ICAM-1阳性表达，随着肝损伤的加重，OVCs继续增生，并且，OVCs沿增生的肝纤维向肝小叶迁移，同时，ICAM-1阳性表达也继续增多，并逐渐向肝小叶弥散分布；透射电镜下观察到，肝损伤初期，OVCs在Hering管周围增生，肝纤维随之增生，增生的OVCs与肝纤维黏附生长。

Electron microscope showed finely granular cytoplasm in group after bFGF stimulation. Most cells lost cytoplasmic organelles. The cells of control group had vast vacuolation of organelles.

电镜下对照组细胞胞浆内有大量肿胀的细胞器，而bFGF组细胞胞浆呈均一细小的颗粒，密度一致，细胞器大部分消失。

Confocal laser scanning microscopy used to localize the hsp70 protein in oocytes and embryos revealed that the distribution of hsp70 in the ooplasm of immature and mature oocytes is unaffected by exposure to decreased or elevated temperatures and that this protein was closely associated with the meiotic spindle indicating its possible role in stabilizing this structure, hi eight-cell embryos derived under control conditions, hsp70 was evenly distributed in the cytoplasm but appeared as aggregates in some embryos exposed to elevated temperature.

此蛋白均匀地分布在两种细胞的胞浆中。此形态特点与减数分裂期纺缍体有关，由此表明hsp70在中期Ⅱ期，具有稳定这种结构的作用。在对照组8细胞胚中，hsp70均匀地分布在胞浆中，而在处理组一些胚胎内，hsp70则发生凝集。

The cell in acidic situation presented a degenerative chang-ment,such as an increased lipid drop and empty cavity in the cell plasma,a py-knotic nuclei,the dilatation of the endoplasmic reticulum and Golgi saccules,mi-tochondrial swelling the number of ribose decrease,and the ribose dissociated inthe plasma,a lot of swallowing cavity,the fibric material decreased accompany-ing with the acid being stronger.

随培养基pH逐渐下降，上述过程因受酸性阻抑而延缓，细胞增殖速度也受影响，处于酸性环境中的软骨细胞呈退行性变化，表现为细胞浆内脂质颗粒与小泡增多、细胞核皱缩、核仁浓染、内质网线粒体膨胀、核糖体数目减少，且与粗面内质网结合的少，游离在胞浆中的多。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。