内胞浆

The typical apoptotic morphological features appeared in MUTZ1 cells treated with 4 mmol/L VPA for 72 hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dosedependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dosedependent manner, the cells were arrested at G0/G1 phase.

结果显示： VPA对MUTZ1细胞的生长抑制作用呈现时间和剂量依赖性；经4 mmol/L VPA处理MUTZ1细胞72小时后，细胞呈现典型的凋亡形态特征，光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体；透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加，胞浆内大小不规则的染色质团块；流式细胞术结果表明，细胞凋亡率随着VPA浓度的增加而逐步增高，G0/G1期细胞比例随着VPA浓度的增加而逐渐增多，S期细胞比例逐渐减低，细胞被阻滞在G0/G1期。

Continuous static load with the same intensity and load amount will cumulatively damage microstructure of skeletal muscle. Observing in general histology, where connecting with perimysium muscle fibre nucleuses increase and muscle fibre denaturalization is familiar.

5在骨骼肌静力性负荷所致损伤过程中胞浆、线粒体和肌浆网均出现明显的钙含量异常增加现象，但并未发现线粒体、肌浆网对钙摄取能力降低，说明肌细胞膜损伤、通透性增加引起的Ca2+内流是导致肌细胞内钙异常增加的主要原因。

The induced hmscs-tert were examined by oil red-o staining, alkaline phosphates staining and von kossa staining. results the hmsc-tert was negative for cd34, cd45, cd44, cd106 and cd166. after the induction, accumulated cytoplasmatic red lipid droplets were clearly visible in the adipose cells by oil red o staining, and alkaline phosphates-positive pitchy grains formed in the intracytoplasm and calcium nodes were found by von kossa staining in the osteoblasts.

结果 hmsc-tert细胞表面抗原检测cd34、cd45、cd44、cd106、cd166均为阴性；脂肪细胞定向诱导后油红o染色见胞浆内有红色脂肪颗粒，成骨细胞定向诱导后见胞浆内有碱性磷酸酶染色阳性黑褐色颗粒，von kossa染色见有钙结节形成。

The frequencies and intensities of the pulse producing bio-effects were dispersive and intervallic.

同时，能够在细胞胞浆Ca^2＋浓度的时-频域内产生生物学效应的电磁波脉冲频率和脉冲强度是离散的和间隔的，这意味着，ELF脉冲电磁波能够在细胞胞浆Ca^2＋浓度的时-频域内产生生物学窗效应。

Their shapes were diversiform such as nummular, elliptic and oval etc. Positive neurons had one or several tubers and its axon was short. This experimentation results showed that development level of eNOS immunopositive neurons approach the adult rabbit when it is a young rabbit.

eNOS阳性神经元呈棕褐色，免疫阳性物质主要位于胞浆内，胞浆染色均匀，胞核处着色浅；神经元胞体形态各异，有圆形、卵圆形、椭圆等多种形状，神经元突起有一个或者数个，轴突较短。

The pseudopodial movement could be inhibited by calcitonin (100 μg*L－1). Lacuna formation could be found in cocultured specimens. There were fibrils on the walls of lacunae. The MGCs demonstrated positive reaction with Gomori stain. Conclusion: The results demonstrated that MGCs had the same morphological features and the bone resorption ability as the osteoclasts.

结果：倒置相差显微镜下可见多核巨细胞胞核较多（20个以上），胞浆周边不规则，有伪足样突起；胞浆内可见较多大小不等的空泡；降钙素（100 μg*L－1）可抑制多核巨细胞的伪足样运动；多核巨细胞与灭活的骨片共同培养时可见骨吸收陷窝形成，扫描电镜下可见吸收陷窝底面有原纤维；Gomori染色时可见多核巨细胞的酸性磷酸酶呈阳性。

Pathologic examination included: tubulointerstitial lesion which was the main pathologic presentation, tubular epithelial cell becoming flat, TEC necrosis and wither, departure between the cytoplasm and basis membrane, drops of protein in cytoplasm, broken basic membrane of TEC, inflammatory cells infiltrating in tubulointerstitium, tubulointerstitium fibrosis, plasm cell infiltration.

MM肾损害主要以小管-间质病变为主，病理表现为肾小管上皮细胞扁平，存在不同程度变性、坏死或萎缩，胞浆与基膜分离，胞浆内可见蛋白滴，甚至小管基膜断裂，间质炎性细胞浸润、纤维化，部分有淀粉样物质沉积，较少见浆细胞浸润。

RESULTS AND CONCLUSION: Osteoblast was fusiformed-shaped and had plentiful processes. Nucleus was orbicular-ovate and leaning to lateral side. Soma was large, and plasma was abundant. Alkaline phosphatase staining suggested that a great number of gray-black particles were observed in plasma, and some region was darkly stained.

结果与结论：成骨细胞呈梭形，多突起；细胞核呈卵圆形，偏于一侧；胞体大，胞浆丰富；碱性磷酸酶染色可见胞浆中含有大量的灰黑色颗粒，某些部位染成黑色，定量分析细胞内的碱性磷酸酶、骨钙素水平明显高于成纤维细胞；细胞免疫组织化学染色表明其主要合成Ⅰ型胶原。

ISHH showed that many positive particles were seen in plasma of many T cells surrounded the blood vessels during 5 dpi～30 dpi and also a little positive particles were seen in plasma of kupffer cell during these time.

原位杂交显示：着染部位主要出现在坏死灶或血管周围的成团或散在的淋巴细胞的胞浆内，少数出现在枯否氏细胞的胞浆内。

Results 1、 Generally, we can see the original blue and white, shiny, no cracks in the articular surface of the cartilage after the stress increases gradually yellow, surface roughness, cracks appear; when the pressure decreases, the yellowing, rough, the color of the fracture restore gradually and become shiny.2、the shiny smooth surface can be seen under a light microscope, formation, cell distribution, tidy, clear the level of cartilage at the articular surface stress increases, the surface roughness changes, defects, disordered cells, uneven dyeing ; when the articular surface of the pressure gradually decreased, the cartilage gradually repair and the surface of cells at the surface appear only disorder.3、immunohistochemical observation can be seen throughout the observation period, cartilage cells are type Ⅱ collagen expression and expression after 3 weeks gradually weakening, when the seventh week begin to strong gradually.4、 electron microscopy shows that when stress increases the articular surface, the cartilage cells became flat, the cytoplasm in the endoplasmic reticulum, Golgi apparatus decreased with collagen disorders; and when stress decreases the articular surface, cartilage cells gradually returned normal, cytoplasm in the endoplasmic reticulum, Golgi body gradually restore quantity; collagen fibers with a gradual rules.

结果：①大体观察可见到原本蓝白色、有光泽、无裂纹的软骨在关节面压力增大后，逐渐呈灰黄色，表面粗糙，出现裂隙；当压力逐渐减小后，变黄、粗糙、有裂隙的软骨颜色逐渐恢复，变得有光泽②光镜下可见表面光滑、平整，细胞分布均匀、整齐，层次清楚的软骨在关节面压力增大后，表面变粗糙、缺损，细胞排列紊乱、染色不均；当关节面压力逐渐减小后，软骨表面逐渐修复，细胞仅在表层排列紊乱③免疫组织化学观察可见整个观察期内软骨细胞胞浆内均有Ⅱ型胶原表达，术后3周内表达逐渐变弱，从第7周时开始逐渐变强。④电镜下可见当关节面压力增大后，软骨细胞逐渐变扁，胞质中内质网膜、高尔基体减少，胶原排列紊乱；当关节面压力减小，软骨细胞形态逐渐恢复正常，胞质中内质网膜、高尔基体数量逐渐恢复；胶原纤维排列逐渐有规则。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。