内种皮

We got the result: Bufo Raddei Strauch Tadpole (1) Bufo Raddei Strauch Tadpole was capable of lens regeneration, which originated from the epithelial cells at iris dorsal margin by dipigmentation.(2) Dipigmentation of Bufo Raddei Strauch by two methods: one was licked up by amoebocyte, the other one was flowed from the pigment cells.(3) In the lens regeneration cells of Bufo Raddei Strauch profiles of the mitochondria became complicated ribosome manifold, and the endoplasmic reticulum was cisternal dilation. During the process of deferentiation of lens fiber the cell nucleolus became little and chromatin shrinked.(4)The crystalline electrophoresis of Bufo Raddei Strauch Tadpole lens regeneration showed that during lens regeneration P-crystalline was expressed earliest then was γ-crystalline and α-crystallin, this result is consistent with newt.

结果表明：花背蟾蜍蝌蚪(1)具有晶状体再生的能力，其晶状体的再生来源于虹膜背缘的上皮细胞；(2)虹膜背缘去色素是转分化的前提，主要有两种方式：一种也是最主要的方式是在晶状体被摘除后虹膜外侧产生巨噬细胞，巨噬细胞吞噬虹膜色素上皮细胞中的色素颗粒；另一种方式是虹膜色素上皮细胞自身释放色素颗粒；(3)电镜观察发现虹膜色素上皮细胞转分化过程中线粒体有明显变化，核糖体增多，粗面内质网增多，晶状体纤维分化过程中细胞核逐渐凝聚；(4)SDS-PAGE电泳发现，花背蟾蜍蝌蚪晶状体再生中相关蛋白表达顺序与有尾类蝾螈相似，蛋白表达顺序依次是β-晶状体蛋白、γ-晶状体蛋白和α-晶状体蛋白，并且后两种蛋白几乎同时表达。

Under light microscope, on H-E stain section, four types cells can be distinguish: cells with weak basophilic fibrillar elements; cells with acidophilic granular substance; cells with strong basophilic fibrillar elements and ciliated cells. In the basal lamina region under gland epithelium, there are a few connective tissue; Surface view of the hypobranchial gland could be see by scanning electron microscope, there are cilia and different kinds of secretions distributed. Ultrastructure of the hypobranchial gland could be understand by transmission electron microscope, supporting cells, sensory cells and seven types gland cell were observed to form the glandular epithelium; cells with much rough endoplasmic reticulum,smooth muscle fiber and nerve endings were found beneath glandular epithelium, among basal lamina region.

光镜下H-E染色切片中腺上皮区仅可以区分出四种类型的细胞：弱嗜碱性纤维样细胞、强嗜碱性纤维样细胞、嗜酸性颗粒分泌细胞和纤毛细胞等；腺上皮下的基底膜区有少量结缔组织存在；扫描电镜下可以观察到鳃下腺表面的纤毛及腺细胞的分泌物等情况；透射电镜下观察到腺上皮中有支持细胞、感觉细胞和7种类型的腺细胞；近基底膜区观察到富含粗面内质网的细胞；基底膜间为薄层疏松结缔组织，内含肌细胞及神经末梢等结构。

In some cases , there are two or more epithelium types which transmigrate each other . Visceral muscle , mucous gland and other components within the cyst wall can be found in some cases .

结果：囊肿壁上皮可为单层或复层扁平，立方，柱状上皮；一些病例可见两种以上上皮组织成分，并相互移行；某些病例，在囊肿壁内可含有黑色素，软骨，平滑肌，粘液腺等多种组织成分。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

The results shown that:(1) Bufo raddei Strauch tadpole was capable of lens regeneration, which originated from the epithelial cells at dorsal iris margin by depigmentation.(2) Depigmentation of Bufo raddei Strauch during lens regeneration was by two methods: First, pigment granules initially dispersed all over the cytoplasm were moved towards the periphery of the cell, and then were directly taken up by the amoeboid cells. Second, pigment granules in a cell were first crowded in a mass, surrounded by a membranous structure in the cytoplasm, which were eventually discharged as a mass from the cell.

结果表明：花背蟾蜍蝌蚪(1)具有晶状体再生的能力，其晶状体的再生来源于虹膜背缘的上皮细胞；(2)虹膜背缘去色素是转分化的前提，主要有两种方式：一种也是最主要的方式是在晶状体被摘除后虹膜外侧产生巨噬细胞，巨噬细胞吞噬虹膜色素上皮细胞中的色素颗粒；另一种方式是虹膜色素上皮细胞自身释放色素颗粒；(3)电镜观察发现虹膜色素上皮细胞转分化过程中线粒体有明显变化，核糖体增多，粗面内质网增多，晶状体纤维分化过程中细胞核逐渐凝聚。

The results showed that: with the regression analysis of Betula platyphylla, Pinus koraiensis, Abies nephrolepis, Picea koyamai var koraiensis, and Betula costata, there was a significant negative correlation between neighborhood interference index and the growth (0.40~0.89) and the correlation extent depended on the neighborhood influence zone and slope. The neighborhood influence zone corresponding to maximal regression coefficient R~2 was the neighborhood influence radius. Based on the linear subsection regression between neighborhood interference index and influence zone of five species, the neighborhood interference index increased with the increasing zone and there was a previous increasing trend in a certain scope, while the increasing trend slackened beyond the zone. The neighborhood influence zone corresponding to transition point was the neighborhood influence radius. The neighborhood interference index positively correlated with the neighborhood influence zone and the range of R~2 was 0.40-0.64. With the analysis of tree cores of Juglans mandshurica, Fraxinus mandshurica at middle and lower slope in a plot which was fostered in 1995, the distance which the growth release farthest neighborhood and object stub was judged, thus the neighborhood interference radius was inferred. According to the studies, we found that the neighborhood interference radius was different among different trees, even if the neighborhood interference radius of the identical tree species also had the difference at different slopes.

结果表明：通过对白桦、红松、冷杉、红皮云杉和枫桦树种进行邻体干扰指数与生长量回归分析发现，邻体干扰指数与生长量呈显著负相关(0.40～0.89)，相关程度因邻体影响范围和坡位而异，R~2达到最大值所对应的影响范围即为邻体影响半径；通过对白桦、红松、冷杉、红皮云杉和枫桦树种进行邻体干扰指数与影响范围的线性分段拟合，邻体干扰指数随影响范围的增加而增加，其在一定的范围内上升较快，而超出该范围后上升的幅度变慢，变化的转折点所对应的影n向范围可以判定为邻体影响半径，线性回归显著(0.40～0.64)；通过对1995a抚育的胡桃楸、水曲柳样树进行研究，分析邻体树种的中坡位和下坡位的生长锥芯，判断产生生长释放的最远邻木与基株树桩所对应的距离，推断邻体影响半径；根据研究发现，邻体影响半径因树种不同有一定的差异，即使同一树种的邻体影响半径在不同坡位也有一定的差异。

The compounds were identified by physical and spectroscopic techniques.Result:Six compounds were isolated and identified as 4,4′-biisofraxidin(1),esculetin(2),fraxetin(3),scoparone(4),isofraxidin(5),scopoletin(6),respectively.Conclusion:Compound 1 is a novel natural product.

结果：首次从草珊瑚干燥全草中分离得到6种具有抗菌活性的香豆精类化学成分，分别为4，4′-双异嗪皮啶(4，4′-biisofraxidin，1)，秦皮乙素(esculetin，2)，秦皮素(fraxetin，3)，滨蒿内酯(scoparone，4)，异嗪皮啶(isofraxidin，5)，东莨菪内酯(scopoletin，6)。

These three internal fixations of femoral peritrochanteric fracture were differ. For example, Gamma nail is less harmful, better clinical treatment, shorter in shape and stronger in anti-hip inversion. DHS, DCS have a better treatment and are stronger in anti-hip inversion but more harmful and excessive bleeding, and HCS, which is named Minimally Invasive Surgery, is the least harmful, less bleeding, suitable for old patients and the patients of tolerance of poor operation, but poor in anti-hip inversion.

3种不同方法内固定治疗转子间骨折具有各自的特点，Gamma钉具有手术损伤小及良好的临床疗效力臂短对抗髋内翻的能力强；滑动加压鹅头钉具有良好的临床疗效及相对坚强的抗内翻能力，但手术损伤相对较大，失血量较大；空心加压螺钉固定的手术损伤最小，属于微创手术方法，可经皮完成，手术出血少，适用于高龄及手术耐受性差的患者，但抗髋内翻的能力最差。

Results Observation of 35 746 grain of airborne fungi spores and 1 188 grain of fungi spores for one year revealed the following species of fungi: Ustilaginales, Aternaria, Uredinales, Clodoporium cladosporioides, Cladosporium, Pithomyces, Cladosporium macrocarpum, Aspergillus niger, Aspergillus versicolor and Aspergillus fumigatus. Thirth-nine of 85 infants and young children with asthma (45.9%) showed positive skin test and 108 of 260 older children with asthma (41.5%) were positive for the skin tests; total positive rate was 42.61%.

结果 经1年的调查，曝片观察到的35 746个真菌孢子和曝皿观察到的1 188个菌落，分属于4个亚门60个种属，常见菌种有黑粉菌、交链孢菌、锈菌、枝孢芽枝菌、芽枝菌、皮思菌、大孢枝孢菌、黑曲菌、杂曲菌和烟曲菌。85例婴幼儿哮喘患儿，39例真菌变应原皮内试验阳性（45.9％），260例儿童哮喘患儿，108例皮试阳性（41.5％），总阳性率42.6％。

RESULT: The K or K of laetrile, paeoniflorin and paeonol had no significant difference at concentration of 19.2~304.8, 18.4~329.5 and 7.8~125 μg/mL respectively or in four different regions of rat intestinal tract, the absorption quantity of laetrile, paeoniflorin and paeonol was proportional to its concentration respectively and the phenomena were not observed saturable. Except for Paeonol, the K and K of laetrile and paeoniflorin had no significant difference at pH value 5.0~7.4. The K or K of laetrile and paeoniflorin from the extration which contains one kind of active components was significantly less than that of same components from GuizhiFuling Capsule content.

结果：苦杏仁苷、芍药苷、丹皮酚分别在19.2~304.8、18.4~329.5、7.8~125 μg/mL浓度范围内，吸收量与浓度呈线性关系，无高浓度饱和现象，K、K值基本保持不变；除丹皮酚外，在pH 5.0至pH 7.4范围内苦杏仁苷、芍药苷的吸收不受pH影响；3成分在各肠段的K、K均无明显差异；仅含有上述3成分中一种成分的各个提取物的苦杏仁苷、芍药苷的K、K值显著小於由配方药材提取得到的胶囊内容物的相应成分的K、K值。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。