内皮细胞

Hepatic sinusoid capillarization plays an important role in occurrence of portal hypertension. The fenestra of endothelial cells in hepatic sinusoid reduces and the diameter of fenestra decreases. The formation of basement membrane impaires the substance exchange between blood and hepatic cells,which results in oxygen deficiency, metabolic product accumulation and cell necrosis.

肝窦毛细血管化在硫代乙酰胺诱导的肝硬化门脉高压发生中起重要作用，肝窦内皮细胞窗孔减少并减小，内皮细胞下基底膜形成使肝细胞与外界物质交换减少，导致细胞缺氧、代谢产物聚集，肝细胞坏死，最终导致门脉高压的发生。

Objective To study the mechanisms and treatment of ischemia /reperfusion injury, expression of intracellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) were measured, the effect on suppression of ICAM-1 and VCAM-1 by the pyrrolidine dithiocarbamate were investigated.

目的 为研究心肌缺血-再灌注损伤的机制和治疗途径，检测血管内皮细胞缺氧/再给氧后细胞间黏附分子- 1(intracellular adhesion molecule1，ICAM- 1)和血管细胞黏附分子- 1(vascular cell adhesion molecule1，VCAM-1)的表达，探讨抗氧化剂吡咯烷二硫氨基甲酸酯(pyrrolidine dithiocarbamate，PDTC)对血管内皮细胞表面细胞黏附分子表达的抑制作用。

The expression of Bcl-2mRNA and P53mRNA in hippocampus after 16Hz, 130dB infrasound treatment In 1 day group, Bcl-2mRNA and P53mRNA were expressed lightly in CA1-CA3 areas in hippocampus and dentate gyrus. Blood vessels dilated lightly. In 7d group, Bcl-2mRNA and P53mRNA were more expressed in areas mentioned above. Dilation of capillaries and small vessels was more obvious. Bcl-2mRNA and P53mRNA were strong positive in endothelial cells of vessels. In 14d group, Bcl-2mRNA and P53mRNA expression was obviously increased in pyramidal cells of hippocampus and granular cells of dentate gyrus, showing deep blue staining. a The endothelial cells in some vessels were also strong positive for Bcl-2mRNA and P53mRNA. Dilation of capillaries and small vessels was lessened. In 21d group, Bcl-2mRNA and P53mRNA expression was decreased in pyramidal cells. In 28d group, little change about the expression and distribution of Bcl-2mRNA and P53mRNA was observed. Lots of Bcl-2mRNA and P53mRNA were scattered in cortex of temporal lobe around hippocampus and callosal gyrus area. Dilation of capillaries and small vessels was lessened obviously. The expression of Bcl-2mRNA and P53mRNA in hippocampus after 16Hz, 90dB infrasound treatment.

次声作用后Bcl-2mRNA和P53mRNA在海马的表达，16Hz、130dB次声作用1d组：两者在海马〓区和齿状回轻度表达，海马内血管轻度扩张；7d组：两者表达在上述区域较1d增多，微血管和小血管扩张较显，血管内皮细胞内可见呈强阳性表达的Bcl-2mRNA和P53mRNA；14d组：海马锥体细胞和齿状回颗粒细胞内Bcl-2mRNA和P53mRNA阳性表达明显增多，呈深兰色，在部分血管内皮细胞内两者强阳性表达，微小血管扩张状态较7d减轻；21d组：锥体细胞胞浆内Bcl-2mRNA和P53mRNA表达减弱；28d组：上述表达及分布与21d组相比无明显改变，海马周围颞叶皮层和扣带回区域亦可见大量散在分布的Bcl-2mRNA和P53mRNA表达，血管扩张状态已明显减轻。90dB次声作用1d-7d后，较对照组无明显变化，14d-21d后轻度表达，28d后，Bcl-2mRNA和P53mRNA表达较强。

The adherent cells from the embryo heart of E10～E12 were constituted by the difform cadiocyte with affluent kytoplasm and the slender endotheliocyte, which arranged in paralleled regularly and was seen easily to stick on the cadiocytes. CD31 antigen was expressed in the adherent of the heart, but not in the stromal cell of fetal liver from E10～E12, which showed uniform morphous with affluent endochylema.

原代心脏贴壁细胞由体积较大、胞浆丰富、呈不规则形的心肌细胞和细长梭形、胞浆较少的内皮细胞构成，容易看到心脏内皮细胞贴附在心肌细胞表面，呈均一的细长梭形排列，表达CD31抗原。

The HBsAg was located in the intervillous space (21 placentas) and...

HBsAg主要分布于蜕膜血管内皮细胞（18例）、蜕膜细胞（21例）、滋养层细胞（8例）、绒毛间质细胞（8例）和绒毛血管内皮细胞（5例）的胞浆和绒毛间隙（21例）中。

Keratin antibody IgM 3B4 specifically against Candida albicans in vitro. METHODS: The different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 were incubated with Candida albicans yeast phase suspension on condition that profited germ tube formation of Candida albicans to observe the action of natural monoclonal anti??keratin antibody IgM 3B4 to Candida albicans germ tube formation.And the different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal anti??

将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4分别与白念珠菌酵母相悬液在有利于芽管形成的条件下共同孵育，倒置显微镜下计数白念珠菌总数以及白念珠菌芽管数；将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4与白念珠菌酵母相和人角质形成细胞混悬液、白念珠菌酵母相和人口腔黏膜上皮细胞混悬液、白念珠菌酵母相和胎儿脐静脉内皮细胞混悬液共同孵育，分别计数人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞上粘附的白念珠菌数。

At 4 wk, the cells in the liver bud migrated into the domain of transversum mesenchyme, where endothelial cells began to form vascular structures, and branched to form a number of hepatic cords.

结果：3wk时靠近肝芽的间充质中的血管内皮细胞多，而肝芽内未见血管内皮细胞及造血细胞：4wk时，肝索形成处可见较多正在形成的新生血管增多，肝索间开始出现少量的造血细胞：5wk时肝索间出现原始的肝血窦结构，血窦内造血细胞的数量较4wk明显增多。

The study ~s conducted to elucidate the mechanism by which the expression of adhesion molecules on human endothelial cells ~s up-regulatecL Methods Human endothelial cells derived fium umbilical venisQ-IUVEC were coincubated in viny whh native human sennn albtuiiin, HSA modified v~ith advanced glycation end products or RPMI-1640 culture medium. The expression of adhesion molecule intereellular adhesion molecule-l(7ICAM-l), vascular cell adhesion molecule-l and E-selectin was determined by irnrnunofluorescen staining and flow cytometer analysis.

分离正常人脐静脉内皮细胞，将晚期糖基化终产物修饰的人血清白蛋白、人血清白蛋白以及RPMI-1640完全培养基与HUVEC在体外共同培养，用荧光单克隆抗体染色，流式细胞仪定量检测内皮细胞表面细胞间粘附分子-1（ICAM-1）、血管细胞间粘附分子-1（VCAM-1）、E-选择素的表达。

Methods:Based on the model of human umbilical vein endothelial cell,this study adopted Rose Bengal Stain, cell ELISA, immunocytochemistry techniques to investigate the effect of berberine on lymphocyte-endothelium adhesion and the molecular mechanism.

以小檗碱处理人淋巴细胞或人脐静脉内皮细胞后，用蛋白染料染色法研究对淋巴细胞与内皮细胞粘附的作用，用细胞ELISA、免疫细胞化学染色法研究对细胞表面粘附分子表达的影响。

CD34+ cells were harvested from G-CSF mobilized adult blood and umbilical cord blood and incubated onto fibronectin/gelatin-coated Petric dishes in the presence of recombinant human vascular endothelial cell growth factorand basic fibroblast growth factor.

毛宁为证明出生后血液循环中存在内皮细胞的前体细胞，从G-CSF动员的成人外周血及脐带血中分离CD34+细胞，将之接种于纤连蛋白与明胶铺板的培养皿上，培养体系中加入重组人内皮细胞生长因子和重组人碱性成纤维细胞生长因子。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷