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The portal of radiocapal joint was in the triangle of tendon of extensor carpi radialis muscle and long abductor muscle of first digit.Lateral to accessory cephalic vein and superficial branceh of radial nerve.The arthroscopic portals in intercarpal joint were in the both sides of extensor carpi radialis muscle.

桡腕关节的进镜部位在关节前方正中偏内侧,在腕桡侧伸肌腱内侧和腕斜伸肌腱之间的三角区,再向内为副皮下静脉及桡神经浅支,腕间关节在腕桡侧伸肌腱的两侧均可。

Small intestinal biopsies showed subepithelial collagen deposition with varying degrees of villous atrophy and varying numbers of intraepithelial lymphocytes. Four patients had previous biopsies showing enteropathic changes without collagen deposition. Seven cases were associated with collagenous colitis and 1 also had features of lymphocytic colitis. Three patients also had collagen deposition in gastric biopsies. One case was associated with lymphocytic gastritis. Celiac disease (CD, gluten-sensitive enteropathy) was documented in 4 patients. Five patients made a clinical improvement with combinations of a gluten-free diet and immunosuppressive therapy. Two patients died of complications of malnutrition and 1 of another illness. Clonal T-cell populations were identified in 5 of 6 cases tested. Four of these patients improved clinically after treatment but 1 has died. Collagenous sprue evolved on a background of CD in 4 cases. There was no history of CD in others and these cases may be the result of a biologic insult other than gluten sensitivity.

小肠活检表现为上皮下胶原沉积,绒毛萎缩程度不一,上皮内淋巴细胞浸润数量不等。4例患者以往曾做过活检,表现为其他肠病改变,并没有胶原沉积。7例患者合并胶原性结肠炎,且1例还有淋巴细胞性结肠炎的特征。3例患者胃活检也发现有胶原沉积。1例患者合并淋巴细胞性胃炎。4例患者有乳糜泻(CD,谷蛋白敏感性肠病)病史。5例患者经无麸质饮食和免疫抑制治疗后,临床症状有所改善。2例患者因营养不良合并另一种疾病而死亡。6例患者做了T细胞受体基因重排,其中5例发现有克隆性T细胞群,这5例中有4例治疗后临床症状加重,且1例死亡。4例患者在CD的基础上病发胶原性口炎性腹泻;其余患者无CD病史,他们的发病也许是生物源性损害,而非谷蛋白敏感。

2The anterior cutereous branches of the obturator artery penetrate deepfascia of thigh and epimysium of the adductor muscles closing against the lower rami of pubisor at its outer margin.Pudendal-thigh skin flap pedicled on it may be elevated to repairrectovaginal fistula or small skin defects of perineum.As its piercing point is much higher andthe location of pedicle is more fixed because of restriction of pubic rami,it is not suited toreconstruct new vagina.Moreover,it is not appropriate naming the obturator aretry(or themedial femoral circumflex artery)the pudendal-thigh artery.

闭孔动脉前皮支紧邻耻骨下支或于其外侧缘,穿大腿深筋膜和内收肌肌膜浅出,以之为蒂可以形成皮下蒂阴股沟岛状皮瓣,用于阴道直肠瘘或会阴部较小皮肤缺损的修复,但由于闭孔动静脉前皮支浅出点位置较高,并且由于耻骨下支的阻挡和限制,致使血管蒂的位置较为固定,以之为蒂形成的阴股沟皮瓣不适宜用于阴道再造;另外,将闭孔动脉命名为阴股沟动脉,也有欠妥帖。

Methods The tissues of cervical carcinoma with HPV16/18positivity was injected into the hypodermis of nude mice to make tumors and transmission,then Ad-p53gene was injected into the tumors to investigate the effect,while DDP,BLM and normal saline were injected into the tumors to as control groups.

将含HPV16/18型感染的宫颈鳞癌组织注入裸鼠皮下成瘤、传代,将以缺陷型腺病毒为载体的P53基因(Ad-P53)瘤内注射,同时用抗癌药DDP、BLM以及生理盐水作对照,观察P53基因治疗宫颈癌的有效性。

In our study, we demonstrated that adult Balb/c mice were susceptible to TOS when infected intracerebrally or subcutaneously s.c.

我们的研究证实,当BALB/c小鼠被神经适应性的托斯卡纳病毒感染脑内或皮下时,它表现为易感的。

Histological structures of bursas of Fabricius were observed at the different developmental stages from chicken embryoes to chickens. The results were as follows. The bursa of Fabricius began to develop on the 13th day of post-hatching and its cell aggregated into the nodosity clique underneath the epithelium mucosae. On the 15th day of post-hatching, those cell cliques were surrounded by the flat epithelium and formed the basic structure of folliculus lymphaticus. The number of lymphocytes in the folliculus lymphaticus increased gradually, and the medulla and cortex of a majority of folliculus were not discriminated obviously. Until on the 7th day for chickens, the medulla and cortex of folliculus formed completely and the number of folliculus increased significantly. From 21 to 35 days old, the folliculus lymphaticus transformed from polygon into rectangle. Mucosa epithelial cells increased gradually and their cytoblasts arranged compactly into aline in the basilar part on the 35th day.

本研究观察了从鸡胚到出壳后的不同生长发育阶段法氏囊的组织结构,结果表明:胚胎13日龄时,法氏囊开始发育,粘膜上皮下形成结节状细胞集团;胚胎15日龄,聚集的细胞集团形成初始的淋巴滤泡;胚胎18日龄到出壳后1日龄,滤泡内淋巴细胞数量增多,多数滤泡髓质和皮质界限仍不明显;7日龄雏鸡法氏囊髓质皮质分界清晰,滤泡数量显著增多;21日龄到35日龄,滤泡由多边形逐渐转变成长方形;在35日龄时,粘膜上皮细胞逐渐增多,排列紧密,细胞核紧密排列成直线位于基底部。

Methods Surgical specimens/cell suspensions wore transplanted into the various tissues of BALB/c node mice (subcutis, cellular orthotopic injection into renal capsule, perinephrium, surgical orthotopic implantation into renal capsule). Tumorigenicity and metastasis were subsequently cvaluated. Immunohistochemical analysis was employed to determine the expression of VFGF, bFGF, P16, Bcl-2 and C-met in the metastatic and non-metastatic RCC xenograft.

采用皮下移植法、细胞悬液原位注射法、肾周筋膜内法、组织块原位移植肾包膜下法对BALB/c裸小鼠接种RCC组织块,观察各种方法的成瘤及转移情况,并采用免疫组化方法初步对比分析了VEGF、bFGF、P16、Bcl-2、C-met在裸小鼠体内的转移性和非转移RCC的表达情况。

appropriately preoperational evaluation and correct operation during and after operation could decrease the incidence rate of flap necrosis and subcutaneous fluidify of the elderly breast cancer patients.

通过合理的术前评估和正确的术中术后操作可以降低老年乳腺癌患者术后皮瓣坏死和皮下积液的发生率,同时积极的术后治疗可以短期内取得良好疗效。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank,选择包含人主要T细胞抗原表位序列的人PSCA基因片段,应用异种化抗原设计技术,保留人T细胞抗原表位,设计异种化PSCA融合抗原片段;(2)根据核酸序列按中心模板法设计引物,应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因,以PCR、限制性酶切和DNA序列测定法进行鉴定:(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点,采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4),并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中,转染293T细胞,借助免疫荧光+流式细胞术考察插入片段表达效率,最终选定PSCA_3片段进行下一步研究;(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下,插入pVAX1-neo-IRES—GM/B7载体中,构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB,并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况;(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞,制备小鼠前列腺癌模型,并采用股四头肌肌肉注射+电脉冲法(Electroporation,EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB,同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照,通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率,来评价该DNA疫苗在小鼠体内的抑瘤效果。

Local production of reactive oxygen species after subepithelial immune complex deposition leads to the formation of lipid peroxidation adducts, which ultimately alter the composition of the glomerular basement membrane.

上皮下免疫复合物沉积后生成的活性氧簇局部产物导致脂质过氧化物生成的内收,并最终使肾小球基底膜的组成发生改变。

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