内皮下的

The other parts of the body beneath the skin as something other than bones, even if there is subcutaneous bleeding, do not go outside the drum skin, packets, but rather to the formation of subcutaneous soft tissue inside the mass.

在下方的多不是骨，皮机构的，其他部分即使有皮下出血，不要外出败鼓皮丸，包，而是内的皮下软组织群众形成。

Under light microscope, on H-E stain section, four types cells can be distinguish: cells with weak basophilic fibrillar elements; cells with acidophilic granular substance; cells with strong basophilic fibrillar elements and ciliated cells. In the basal lamina region under gland epithelium, there are a few connective tissue; Surface view of the hypobranchial gland could be see by scanning electron microscope, there are cilia and different kinds of secretions distributed. Ultrastructure of the hypobranchial gland could be understand by transmission electron microscope, supporting cells, sensory cells and seven types gland cell were observed to form the glandular epithelium; cells with much rough endoplasmic reticulum,smooth muscle fiber and nerve endings were found beneath glandular epithelium, among basal lamina region.

光镜下H-E染色切片中腺上皮区仅可以区分出四种类型的细胞：弱嗜碱性纤维样细胞、强嗜碱性纤维样细胞、嗜酸性颗粒分泌细胞和纤毛细胞等；腺上皮下的基底膜区有少量结缔组织存在；扫描电镜下可以观察到鳃下腺表面的纤毛及腺细胞的分泌物等情况；透射电镜下观察到腺上皮中有支持细胞、感觉细胞和7种类型的腺细胞；近基底膜区观察到富含粗面内质网的细胞；基底膜间为薄层疏松结缔组织，内含肌细胞及神经末梢等结构。

The fatty streak, consisting of lipids and lipoid proteins, located in the intima of the vessel with the overlying endothelium intact, is the earliest form of atherosclerosis.

脂纹在正中，位于完好血管内膜的内皮下，其成分为脂类和脂蛋白，是最早期的动脉粥样硬化。

Result of HE and special dye1.1 result of HE More cells are found in subsequent cholesteatoma. Cholesterol crystal, both sequestrum and neogenesis bone , gland metaplasia, Polykaryocyte are found in subepidermis of subsequent cholesteatoma.

结果1、HE染色结果：可见后天性胆脂瘤上皮的细胞层次增多，上皮内及上皮下有不同程度的急性、慢性炎细胞浸涧；上皮下有时可见胆固醇肉芽肿、死骨与新骨形成、腺体化生等病理改变。

Firstly,all patients have been excided the upper ductus choledochus,the liver gross duct,left right liver duct besides one centimeter of liver duct that lies upwards the hepatolith.Secondly, we have sutured the excided bile duct, which has been shaped acetabuliform,lifted one part of jejunums,intered non-port one subcuneously,and then inosculated gall with enteron by Roux-en-Y way.Lastly,we have partly excised the ill liver in regular way.Results 26 ones have been follow-up for 1～10 years.The rate is 81 percents,average for 6 years.

32例肝内胆管结石均接受皮下盲袢型胆肠吻合术，其中结石位于右肝7例（22%），左肝9例（28%），两肝均有16例（50%）；结石合并肝胆管狭窄24例（75%），全部病例均切开胆总管上部、肝总管、左右肝管，并切开肝内胆管狭窄部以上1cm肝管，将切开的胆管做必要的缝合，形成碟状，取一段空肠上提，盲袢埋置皮下，再做胆肠Roux-en-Y吻合，病肝做规则性肝部分切除术。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

The subcutaneous anteromedial aspect of the tibia is an excellent source for such grafts.

位于皮下的胫骨前内侧面是提供此种植骨极好的部位。

Results:(1) CBC possesses a natural trabecular structure. Its components is similar to the mineral of bone.(2) CBC has great cellur biocompatibility. It can promote cell adhesion, proliferation and secretory while needn t modification.(3) In nude mice, the ectopic osteogenesis of MCCAB was obvious which was better than that of CBC alone and the new bone formation increased in pace with time. In the MCCAB, both intramembranous and cartilaginous osteogeneses were seen but the former dominated.(4) When MCCAB was autotransplanted intramuscularly, the osteogenesis was much better than that of negative control groups.(5) The MCCAB can repair autogenous calvaria defects effectively and the effect was much better than that of CBM alone and blank group. Conclusion:(1) CBC possesses good biocompatibility, osteoconduction and porous structure, can be used as scaffold material for constructing of tissue engineering bone.

结果：(1)CBC具有天然的多孔网状结构，最大程度地保持了骨矿的微结构特征，可以作为骨组织工程的支架材料；(2)CBC细胞亲和性好，不需作修饰即能促进成骨细胞的粘附、增殖和分泌活动；(3)MSO/CBC复合人工骨在裸鼠皮下异位成骨作用明显，兼有膜内成骨和软骨内成骨的特点，成骨效果明显优于单纯CBC植入组；(4)MSO/CBC复合人工骨兔自体肌袋异位成骨作用明显，血管化充分，成骨效果明显优于单纯CBC植入组；(5)MSO/CBC复合人工骨修复兔颅骨临界性缺损效果好，明显优于单纯植入CBC组和空白对第四旱区大学硕士学位论文一照组，可以作为骨移植替代材料用于骨缺损畸形修复。

The RF electricity of ice adopts radio frequency technique of the RF dual pole. The frequency can make the water element of the cell which at the certain depth shake at a high speed, it can produce the quantity of heat at 45-60℃ through the quick circumvolved friction. Because the collagen fibre is shrinked, the wrinkle can be tighted. Then the light pyrolysis stimulates collagen and elasticity fibre to become more and arrange once again so as to reach the function of dispelling wrinkle and shrinking pore.

冰电除皱系统采用RF双极射频技术，发射的频率可以在皮下的特定深度内使细胞内的水分子产生高速震动，通过这种高速的旋转摩擦从而产生热量45-60℃，胶原纤维收缩，即刻松弛的皮肤、皱纹被拉紧，随后，真皮层中产生的热效应使胶原增生、重新排列、数量增加，同时修复老化受损的胶原，达到即刻紧肤、长效除皱抗衰老的效果。

Methods Cut the scar along it's length with operation knife to form a scar-skin which thickness is of about 0.8~1.2mm,then cut off the scar tissues under the scar-skin,replant the scar-skin in the same place,enswathed the wound with proper pressure.

沿瘢痕纵轴在瘢痕内切口，手术刀锐性剥离，形成0.8～1.2mm厚的瘢痕皮，切除瘢痕皮下的瘢痕组织，将瘢痕皮原位回植，适当加压包扎。5～10天拆线，应用肤康霜或硅酮霜3～6个月。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！