内皮

NO may be one of the important molecular regulators to permeability of strial capillary endothelial cells by TNF-α,and changes of F-actin content probably relate to the regulation in these cells.

TNF-α能增加耳蜗血管纹微血管内皮细胞的通透性，NO可能是TNF-α调控内皮细胞通透性的重要分子机制之一；耳蜗微血管内皮细胞中F-acfin含量的改变可能与其通透性的调控有关。

Methods Strial capillary endothelial cells in guinea pig cochlea was dissociated and cuhureed to establish a model for its permeability in vitro.,The animals were divided into TNF-α,TNF-α+L-arginine,TNF-α+NG-minomethyl-L-arginine and control groups.

体外分离培养豚鼠耳蜗血管纹微血管内皮细胞并建立内皮细胞通透性体外模型，观察TNF-α、TNF-α连同L-精氨酸(L-arginine，L-Arg)或L-单甲基精氨酸(NG-monomethyl-L-arginine，L-NMMA)对内皮细胞通透性的影响。

These include physical characteristics, such as repulsive forces resulting from the electronegative charge of both platelet and endothelial cell membranes, and biochemical attributes, such as the synthesis and release of inhibitors of platelet activation. Platelets respond rapidly to alterations of endothelial cells and to exposure of subendothelial structures by attaching firmly to the site of the lesion.

正常情况下，循环中的血小板与血管壁内皮细胞层紧密接触但不发生黏附，是因为血管内皮细胞具有一定的活性功能，包括一些物理特性如血小板和内皮细胞膜均带有负电荷产生相互排斥作用以及生化作用如合成和释放血小板活化抑制剂。

Immunofluorescence assay was used to detect endothelial cell uptake and absorption acetylated low density lipoprotein and ulex europaeus agglutinin-Ⅰ. EPCs quantitative detection of surface markers was performed by flow cytometry: CD133, CD34 antigen expression and KDR.

通过免疫荧光法观察内皮细胞吸收乙酰化低密度脂蛋白和荆豆凝血素Ⅰ情况，流式细胞仪定量检测血管内皮祖细胞表面CD133、CD34和KDR抗原表达阳性率来鉴定血管内皮祖细胞。

It becomes evidently that endothelial progenitor cells are involved in postnatal reendothelization and vasculogenesis. It is suggested that endothelial progenitor cells will have clinical application prospects in vascular regeneration and treatment of heart and vascular diseases.

但近年的研究证实，内皮祖细胞参与出生后的内皮修复和血管新生过程，提示内皮祖细胞在血管再生和心血管疾病治疗中具有很好的应用前景。

Ox-LDL can inhibit the proliferation of EPC and promote the apoptosis of the cells by down-regulation of bcl-2 expression, which may contribute to vasculogenesis and reparation of blood vessel endothelium.

氧化型低密度脂蛋白通过下调bcl-2的表达诱导内皮祖细胞凋亡、抑制内皮祖细胞增殖，这可能影响血管内皮的修复及新生血管的形成。

When cultured in three-dimensional matrix, the cells were elongated and migrated into the gel, with a phenotype similar to that in the process of angiogenesis and vasculogenesis in vivo .

在二维平面培养中，原代心脏血管内皮细胞呈铺路石样排列；而在三维胶原培养模式中，内皮细胞呈长梭状形态，并迁入胶原培养介质中，和体内血管新生及血管生成过程中的内皮细胞活化表型相似。

ET and NO are the biology regulatory factors produced by vascular endothelial cell.ET is a peptide with vasoconstrictive and vasodilator effect produced by endothelial cells,and NO as a powerful endothelium-derived relaxing factor,has a strong role in dilation of blood vessels.

ET和NO是血管内皮细胞产生的生物调节因子，ET是由内皮细胞产生的一种多肽，具有缩血管和扩血管作用，而NO为一种强有力的血管内皮细胞舒张因子，具有强烈扩张血管作用。

However, higher concentration of CRP may increase the expression of protein and mRNA of inflammatory factor in endothelial cells; 2 Higher concentration of CRP may participate in the development of inflammatory process in endothelium by the mechanism that CRP induce cells apoptosis and activate signal pathway involved in cell proliferation. 3 CRP is the member of pentraxins family, in which two faces of pentamer form a special bi-phase structure: one face is ligand recognition phase; the other face is effector phase.

总结： 1CRP是一种保守蛋白，在正常生理水平下对内皮细胞没有明显的诱导炎症发生的作用，仅在高浓度条件下有可能诱导细胞炎症相关蛋白及mRNA的表达； 2高于生理水平的CRP对内皮细胞的致炎作用主要通过诱导细胞凋亡及激活细胞增殖相关蛋白信号通路的表达； 3CRP是一种双面五聚体蛋白，结构具有特殊的二相性，能够分别结合内皮细胞和E-LDL。

At least 0.1μg/ml of endotoxin can induce the morphologic changes and the decrease of F-actin in HUVECs. Endotoxin can injury HUVECs only when its concentration reaches 0.4μg/ml and incubates over 12 hours. It suggests there is a different molecule mechanism in VEC from the endotoxin-CD14 pathway in CD14 positive cells.

内毒素直接损伤血管内皮细胞须较大浓度(＞0.4μg/ml)，但在较低浓度下(0.1μg/ml)可直接引起内皮细胞的一些形态学变化和F-actin含量的变化，初步推测血管内皮细胞可能存在着与通常认识的内毒素—内毒素结合蛋白—CD14途径激活细胞不同的分子机制。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。