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Methods Based on the model of human umbilical vein endothelial cell,this study adopted Rose Bengal Stain,cell ELISA,immunocytochemistry techniques to investigate the effect of APS on lymphocyte-endothelium adhesion and the molecular mechanism.

以体外培养的人脐静脉内皮细胞为模型,应用蛋白染料染色、细胞ELISA、免疫细胞化学等方法,研究黄芪多糖对淋巴细胞与血管内皮细胞粘附的影响及其分子机制。

Methods:Based on the model of human umbilical vein endothelial cell,this study adopted Rose Bengal Stain, cell ELISA, immunocytochemistry techniques to investigate the effect of berberine on lymphocyte-endothelium adhesion and the molecular mechanism.

以小檗碱处理人淋巴细胞或人脐静脉内皮细胞后,用蛋白染料染色法研究对淋巴细胞与内皮细胞粘附的作用,用细胞ELISA、免疫细胞化学染色法研究对细胞表面粘附分子表达的影响。

Methods:Based on the model of Human umbilical vein endothelial cell,Rose Bengal Stain was used to study the influence of herbage containing serum of HLJDT on LPS or TNF-α induced neutrophil-endothelium adhesion by pretreated HUVEC4h、12h、24h.

中文摘要:以体外培养的人脐静脉内皮细胞为模型,应用蛋白染料染色法,观察HLJDT含药血清及其与LPSINF-α共同处理HUVEC4h、12h、24h后,对中性粒细胞与血管内皮细胞粘附的影响。

The model of ECV-304 cell oxidative stress injury was established by hydrogen peroxide (H2O2).Then EPZ-contained blood serum was taken as experimental drug. The adherence of monocytes to endothelial cell were measured by method of rose Bengal. The total RNA of cells was extracted. The intercellular adhesion molecule-1 (ICAM-1),vascular cell adhesion molecule-1 (VCAM-1) and MCP-1 mRNA expression in cells were detected by RT-PCR.MCP-1 protein expression were detected by ELISA.

采用过氧化氢建立离体培养的血管内皮细胞(ECV-304)氧化应激损伤模型,取健康大鼠每日灌服不同剂量的鲜姜有效部位(200,400,800 mg·kg-1)或阳性对照药洛伐他汀(40 mg·kg-1),取含药血清作为受试药物,用孟加拉玫红染色法测定单核细胞与内皮细胞的黏附力;逆转录聚合酶链反应测定细胞内细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子(VCAM-1)以及单核细胞趋化蛋白(MCP-1)的mRNA表达水平;酶联免疫吸附法测定MCP-1蛋白表达水平。

Results There is the antibody ofⅧ-R:Ag and the Weibel-Palade body in the cell plasm of the endothelial cell of the radial artery and the saphenous vein undergoing coronary artery bypass.The cell cultivated was confirmed the endothelial cell.

结果 人冠状动脉搭桥材料桡动脉和大隐静脉内皮细胞胞浆中有Ⅷ因子相关抗原的抗体,电镜下可见胞浆中有W-P小体,证实培养的细胞为血管内皮细胞。

The parallel plate flow chamber apparatus was established to study the relationship between the endothelin metabolism of capillary endothelial cells and duration of shear stress action.

应用平行平板流动腔装置研究毛细血管内皮细胞内皮素代谢与剪切作用时间的相关关系。

To investigate the mechanism of acupuncture on cerebral ischemic vascular diseases,we have studied the expression of endothelial functional corelated genes in the rats cerebral microangium .

为了进一步探讨针刺治疗缺血性脑血管疾病的机理,从脑微血管内皮细胞的角度,进行了针刺对MCAO大鼠脑微血管内皮功能相关基因表达影响的研究。

On the other hand, we also analyzed and identified the proteins with changed expression upon the stimulation with carbachol in the cultured human coronary artery endothelial cells.

在10一7mol.L一'5一HT预收缩平衡的内皮完整的脐静脉血管环上, ATPlo一'mol 。L一'可以诱发用的血管的内皮依赖性舒张反应。

RESULTS:①Endothelial chimerism was common in renal transplantation.

结果:①肾脏移植物中血管内皮细胞替代现象存在较为普遍,17例移植肾出现内皮细胞替代。

RESULTS:①Endothelial chimerism was common in renal transplantation. Seventeen cases developed endothelial chimerism.

结果:①肾脏移植物中血管内皮细胞替代现象存在较为普遍,17例移植肾出现内皮细胞替代。

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