内皮

The platelets were labeled by FITC injected by jugular artery. Adherence of platelets to endothelial cells in pial vessel was observed by fluorescent microscope. The expression of GPⅢa protein was identified by CD61 histochemistry.

荧光显微镜下观察活体软脑膜微血管中血小板对内皮细胞的粘附，用CD61抗体经免疫组化方法观察缺血再灌注沙土鼠脑组织血管内血小板和内皮细胞上GPⅢa糖蛋白的表达。

This hindrance is thought to be due to limited proliferative capacity of the endothelial layer.

产生这一障碍的原因是内皮细胞层的功能受到抑制或内皮细胞的增殖受到抑制而无法修复损伤。

Methods Pterygial samples were extracted and collected and the pterygial endothelial cells and pterygial fibroblasts were cultured alone, conditional and co-cultured to form different culture systems. The methods included that to select the suitable intensity of ultraviolet by MIT, to detect the changes of curves of growth about two kinds of cells by MIT and to explore the developments of protein and RNA of vascular endothelial growth factor and fibroblast growth factor-basic in three culture systems under ultraviolet whose intensity is 20 mJ/cm^2 by ELISA and RT-PCR.

收集翼状胬肉标本，采用血管内皮细胞和成纤维细胞单独培养、条件培养和共同培养的方法构建体系，用MTT法选择紫外线照射细胞的适宜强度；采用MTT法绘制强度20mJ/平方公分紫外线照射下细胞生长曲线；采用ELISA和RT-PCR检测紫外线照射下3种体系中细胞上清液和细胞中血管内皮细胞生长因子和成纤维细胞生长因子的蛋白和RNA含量变化。

Expression of ET-1 in pulpitis was stronger than that of normal pulp.

结论与正常牙髓组织相比，炎症牙髓组织中内皮素-1表达增强，提示内皮素-1在牙髓炎发展过程中起一定作用。

Transmission electron microscope results revealed vascular endothelial cells with normal morphology in the control group and tumor cells with apparent nucleoli in the Ad-GFP group, while in the Ad-aMMP-2 group, some vascular endothelial cells exhibited chromatin pycnosis in the nucleus, forming apoptotic bodies.

透射电镜观察对组血管内皮细胞形态正常；Ad-GFP组瘤细胞大，核仁明显；Ad-aMMP-2组部分血管内皮细胞核内染色质固缩，聚积成团块状形成凋亡小体。

Objective: Culture, purify and identify the bovine retial endothelial cells in vitro.

第一部分牛视网膜血管内皮细胞的体外培养目的：体外培养纯化并鉴定牛视网膜血管内皮细胞。

Methods:(1) Silica column chromatography and LH-20 were used in phytochemistry study.(2) Based on the extraction ratio of arctiin, the best extraction procedure was acheived under the direction of L9(34) orthogonal experimental design. From 10 types of resins, the one with the maximal binding ability for Arctii was selected and silica-column choramatograpy was used in its seperation and purification procedure.(3) The animal diabetic experimental model in Wistar rats was established with streptozotocin and many biochemical factors were used to indicate the pharmacological effects of arctiin, including glycosylated hemoglobin, serum creatinine, seralbumin, monoxide nitrogen, glycerin trilaurate, cholesterol, HDL-ch, LDL-ch, Blood urea nitrogen, creatinine, SOD, ET, MDA, total Urine protein and albuminuria.

(1)采用硅胶柱层析、聚酰胺层析、LH-20等方法对牛蒡子的化学成分进行研究；(2)采用正交实验设计方法，以牛蒡子苷为指标，确定了牛蒡子乙醇回流提取的最佳工艺；对10种类型大孔吸附树脂进行筛选，筛选出对牛蒡子苷吸附效果最佳的树脂；采用硅胶柱层析法，对牛蒡子苷进一步分离纯化；(3)建立实验性糖尿病大鼠模型，以GLU、TC、TG、HDL-ch、LDL-ch、TB、ALB、BUN、CREA、GHbAlc、NO、SOD、ET、MDA和尿液中TB、ALB为指标，研究牛蒡子苷药理活性；(4)以差速消化法纯化Wistar大鼠的主动脉内皮细胞并进行传代培养，测定牛蒡子苷对高糖条件下RAECs活性、RAECs释放LDH、MDA和NO的变化以及RAECs表达内皮型一氧化氮合酶表达的影响。

Being as a NO donator,L-Arg siguitlcanfly enhanced the ability of TNF-α which increasing permeability of strial capillary cndothelial cells;permeation rate to Evans blue beightened significantly(P＜0.01),which obviously rose up when the concentration of Evans blue added.L-NMMA,NO inhibitor,was able to weaken the ability of TNF-α which increasing permeability of strial capillary endothelial cells:permeation rate to Evans blue decreased siguificantly (P＜0.01),which obviously fell down when the concentration of L-NMMA added.

一氧化氮(nitric oxide，NO)供体L-Arg能增加TNF-α作用下耳蜗血管纹微血管内皮细胞的通透性，使伊文思蓝的通透率升高，差异具有统计学意义(P值均＜0.01)，且随着L-Arg浓度的增加内皮细胞对伊文思蓝的通透率也明显增加。

Inhibition of early steps of platelet adhesion to the ischemic endothelium and the subendothelial matrix may offer a novel and safe treatment strategy in acute stroke.

在血小板黏附在血管内皮细胞及内皮下基层的早期进行抑制可能提供一种新的安全的治疗缺血性卒中的治疗方法。

Inhibition of early steps of platelet adhesion to the ischemic endothelium and the subendothelial matrix may offer a noel and safe treatment strategy in acute stroke.

在血小板黏附在血管内皮细胞及内皮下基层的早期进行抑制可能提供一种新的安全的治疗缺血性卒中的治疗方法。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新