内皮

AIM: To observe the effect of endothelin receptor antagonist (BQ123) on calcification in rat vascular smooth muscle cells in vitro. METHODS: Calcification of cultured rat VSMCs was produced by incubation with β-glycerophosphate.

目的：在离体钙化的血管平滑肌细胞上，观察内皮素受体阻断剂对血管平滑肌细胞钙化的影响，探讨内皮素促进细胞钙化的信号转导和分子机制。

METHODS:Calcification of cultured rat VSMCs was produced by incubation with β-glycerophosphate. Calcium content, Ca2+ deposition and alkaline phosphatase activity were analyzed to estimate the extent of calcification.

目的：在离体钙化的血管平滑肌细胞上，观察内皮素受体阻断剂对血管平滑肌细胞钙化的影响，探讨内皮素促进细胞钙化的信号转导和分子机制。

Microarray technique was applied to look into the gene expression profiles. Ox-LDL induced HAEC apoptosis by inducing many redox- and apoptosis-related genes. Sal B prevented the ox-LDL induced endothelial damage and even induced endothelial cell proliferation.

本计划亦探讨丹参酚酸 B 抑制氧化低密度脂蛋白所造成内皮细胞的损伤与细胞附著分子表现的作用机转，亦引入微矩列技术以探讨基因表现差异之谱型，氧化低密度脂蛋白诱导许多氧化还原与细胞凋亡相关基因，丹参酚酸 B 可保护内皮细胞损伤甚至促进其增生。

Human umbilical venous endothelial cells were cultured, and were exposed to different concerntrations of HCY with/or not with CuSO4; Cell counting of detached cells were performed with a hematocytometer; the cell survival was monitored by MTT assay and the cellular injury was evaluated by LDH release; Hoechst 33258 staining was used to observe nuclear morphological changes and the apoptosis was measured by DNA agarose gel electrophoresis ;the percentage of apoptosis was quantified by flow cytometry.

体外培养人脐静脉血管内皮细胞，用不同浓度的HCY伴／不伴生理浓度Cu~(2+)与内皮细胞作用；细胞计数板检测脱落细胞率；MTT法及乳酸脱氢酶释放率的测定来衡量细胞的存活与损伤；Hoechst 33258荧光染色观察细胞核形态变化和DNA琼脂糖凝胶电泳检测细胞凋亡，并采用流式细胞术定量测定细胞凋亡率。

By comparison, the hemogenic endothelium is proposed to characterize the generation of hematopoietic stem cells from mature endothelium in the PSp/AGM region.

在卵黄囊和PSp/AGM区，造血和内皮细胞的发育关系分别表现出不同的特点：二者在卵黄囊共同来源于成血血管细胞；在AGM区则更多表现为生血内皮的模式。

Furthermore, couples of molecules, including Scl, Flk1 and Runx1, are involved in formation and subsequent differentiation of the hemangioblast or hemogenic endothelium during embryonic hematopoiesis.

造血和血管内皮相关分子的表达和调控作用的研究，不仅体现了造血和内皮在发育中的密切关系，同时也加深了对造血发育调控的认识。

Heterogenic DM/posterior stroma layer is a good carrier for culturing corneal endothelium, which provides a donor material for endothelium cells transplantation.

角膜内皮细胞能够在异种DM／基质载体上良好生长，该载体有可能成为培养内皮细胞应用於临床移植的供体材料。

Methods: The human endothelial cells of umbilical vein and rat microvascular endothelial cells were obtained by methods of perfusion/digestion and histiocytic attachment using typeⅠcollagenase (0.1%). The morphologic characteristics of endothelial cells were observed under inverted microscope.

采用0.1%Ⅰ型胶原酶消化灌注法和组织块贴壁法分别获得人脐静脉内皮细胞和大鼠微血管内皮细胞，每天在倒置相差显微镜下进行观察，并用免疫荧光法分别进行鉴定。

Objective To investigate changes of vWF in vascular endothelial cells with acute hypoxia and assess its significance in the study of vascular injury and hypercoagulability.

目的 观察急性缺氧时内皮细胞中血管性血友病因子的变化及其与血液高凝状态之间的关系，确定其在反映血管内皮细胞损伤和血液高凝状态研究中的价值。

Objective To investigate changes of vWF in vascular endothelial cells with acute hypoxia and assess its significance in the study of vascular injury and hypercoagulability. Methods vWF in vascular endothelial cells and in blood were measured by immunohistochemical staining image analysis and ELISA technique.

目的 观察急性缺氧时内皮细胞中血管性血友病因子的变化及其与血液高凝状态之间的关系，确定其在反映血管内皮细胞损伤和血液高凝状态研究中的价值。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新