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Methods Fifteen dogs were randomly divided into 3 groups. Group A acted as the control group without CPB; group B and group C were subjected to cardiopulmonary bypass and crossclamping the aorta for 90 min. MP or saline was injected intravenously before surgery. Plasma levels of IL-1β、TNF-α、IL-6、IL-8 and IL-10 were measured at the following time points: before MP or saline were administered, aortic declamping, 30 min and 90 min, after aortic declamping. Immediately after the animals was killed, pulmonary arterial rings were obtained and mounted in organ chamber for assessment of endothelial function with endothelium-dependent or non-endothelium-dependent studies.

健康家犬15只,随机分为3组,A组:不进行CPB,作为非体外对照组;B组:麻醉后注射生理盐水10mL,常规全麻和CPB,主动脉阻断90min;C组:麻醉后静脉给予甲泼尼龙5mg/kg,其余处理同B组。B、C组分别在手术前及主动脉开放前、开放后30min和90min 4个时间点采动脉血,放免法测定血浆白介素-1β(IL-1β)、肿瘤坏死因子-α、白介素-6(IL-6)、白介素-8(IL-8)和白介素-10(IL-10)水平;手术结束时,A、B、C 3组均取右肺动脉段,固定于张力传感器,测定血管环内皮依赖性和非内皮依赖性扩张能力的变化。

LPS-induced ICAM- 1 expression of endothelial cells possessed time- dependent and dose-dependent characteristics, and tolerated to LPS chronical stimulation.

LPS刺激和内皮细胞表面ICAM-1的表达具有一定的时间和剂量依赖性,内皮细胞ICAM-1的表达对LPS长期刺激可产生耐受性。

Results Many endothelial cells were observed with higher vigor and adherance to the wall after incubation for 3-5h, and spreaded to form cell masses for 12-24h, and grew densely and converged into single layer which characteristically arranged to cobble-stone shape after incubation for 1-2 weeks.

结果 原代细胞培养3~5h后,大部分活力较高的内皮细胞已开始贴壁,12~24h后,内皮细胞己铺展形成由数个细胞组成的细胞群,1~2w后,细胞呈生长密集状并汇合形成单层,在倒置相差显微镜下,呈特征性"鹅卵石状"或"铺路石状"镶嵌排列生长。

In this study, we investigated the migration of Daudi cells across confluent monolayers of the human vascular endothelium cells.

为此,我们观察了CD40分子激发对Daudi细胞与血管内皮细胞的相互作用及其穿越血管内皮单细胞层的影响。

Objective To investigate the expression of vascular endothelial growth factor and vascular endothelial growth factor receptor in lymphoma and the effect of curcumin on them.

目的 探讨淋巴瘤细胞血管内皮生长因子、血管内皮生长因子受体的表达及姜黄素对其表达的影响。

AIM: To investigate the changes of structure and function in regenerated endothelial cells after the denudation of endothelium in rat aortic artery.

摘 要 目的:探索大鼠主动脉内膜球囊剥脱后,内皮修复过程中再生内皮细胞结构和功能的改变。

Results: radiation-induced depolymerization of F-actin microfilament stress fibres was dependent on the radiation dose.

电离辐射对血管内皮细胞骨架蛋白F-actin的破坏可能是血管内皮细胞辐射损伤机体机理之一。

While the'endothelial cells were cultured in the media containing CGRP, the expression of ICAM-1 significantly diminished and the expression of NOS and TIMP-2 raised, especially in ECs induced by diamide.

当CGRP作用于正常内皮细胞和联胺诱导启的内皮细胞,对ICAM-1的表达有明显的下调作用,对NOS和T1MP-2的表达则有明显的上调作用。

METHODS: A limb ischemia-reperfusion injury model in rats was established. The animals were randomly divided into three groups: control group, IR group and IPC group. The contents of diamide oxidase, nitric oxide, endothelin-1 (ET-1) and ratio of nitric oxide/endothelin-1 (NO/ET-1) in the plasma and the gut were measured. The leavels of myeloperoxidase, ratio of DNA chain, total nitric oxide synthase, inducible nitric oxide synthase and constitutive nitric oxide synthase in the gut were determined.

雄性Wistar大鼠18只,随机分为对照组,缺血再灌注组和缺血预适应组,每组6只,分别测定血浆和小肠组织二氨氧化酶、一氧化氮、内皮素-1(ET-1)、NO/ET-1比值的含量变化及小肠组织的髓过氧化物酶、DNA双链百分率(ratio of DNA chain %)、总一氧化氮合酶、诱导型一氧化氮合酶、结构型一氧化氮合酶的水平;免疫组化法检测小肠组织的诱导型一氧化氮合酶、内皮型一氧化氮合酶的表达。

To study the effects of weight reduction on endothelium-dependent dilatation and insulin resistance in obese subjects and to explore the relationship between endothelial dysfunction and IR.

目的 观察减重对肥胖者血管内皮功能及胰岛素抵抗的影响,探讨内皮依赖性舒张功能障碍与IR的因果关系。

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