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One endophytic bacteria strain LP-5 which could significantly inhibit some phytopathogenic fungi was isolated from xylem of pear. According to morphology, physicochemical test, and analysis of the 16S rDNA sequence and specific amplified fragment, the strain was identified as Bacillus amyloliquefaciens.

从梨树木质部分离到1株具有强烈抑菌活性的内生细菌LP-5,通过形态观察、生理生化测定、16S rDNA序列分析及特异基因片段扩增,将其鉴定为解淀粉芽孢杆菌。

Biocontrol efficiency of Bacillus subtilis B6 strain and Trichoderma virid strain T23,in combination,was 82.22% in the greenhouse experiment,and 20.3% and 48.9% higher than treated groups single with B6 and T23 respectively.

在对甜瓜枯萎病温室防效试验的研究中发现,内生枯草芽孢杆菌B6菌株与绿色木霉T23菌株复合处理的相对防效达82.22%,比B6和T23的单独处理分别提高32.8%和146.7%。

The inhibitory effect was strongest in King B2 medium, the optimum temperature for the highest antimicrobial activity was 33℃, and the optimal pH9.0. In the antagonistic test of BEB2 to the banana wilt disease, the inhibition was obvious with different in vitro methods, and the control effect of pot experiment was good.

结果发现,香蕉内生拮抗细菌BEB2为枯草芽孢杆菌;不同温度、pH值和培养基下的BEB2发酵液对香蕉枯萎病菌生长的抑制能力具显著差异,在King B2培养基上抑菌作用最强,最适抑菌温度为32 ℃,最适抑菌pH值为9.0。

Endophytic Bacillus subtilis strain B47,which was isolated from the stem of tomato, was an efficient biological control agent on tomato bacterial wilt. A strain resistant to streptomycin derivative of wild strain B47 was used to study the colonization ability and fluctuation of populations in soil and tomato organs, and its biological control on tomato bacterial wilt.

从番茄茎分离的内生枯草芽孢杆菌菌株B47对番茄青枯病有较好的防治作用,利用该菌株的抗链霉素突变菌株,研究其在土壤和番茄植株根,茎中的定殖能力及其对番茄青枯病的防治作用。

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