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内生芽孢

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The results showed the population of endophytic bacteria from oilseed rape was very abundant.These bacteria species is the first reported in oilseed rape, and Mycoplana bullata、Acinetobacter calcoaceticus、Pantoea dispersa、Pantoea ananas、Enterobacter cowanii、Pseudomonas fulva、Pseudomonas parafulva are new record species of endophytic bactria..

通过16S rDNA 序列系统发育分析对PCR-RFLP 划分的主要类群进行了鉴定,共鉴定出内生细菌16 种,分别是,分别为:蜡状芽孢杆菌、泡状枝动菌、醋酸钙不动杆菌、鲍氏不动杆菌A。

The main groups from PCR-RFLP analysis were identified by 16S rDNA gene sequence phylogenetic analysis.16 species were obtained, they were: Bacillus cereus、Mycoplana bullata 、Acinetobacter calcoaceticus 、Acinetobacter baumannii 、Chryseobacterium indolthetium、Acidovorax avenae、Pantoea ananas、Pantoea agglomerans、Pantoea dispersa、Pantoea spp.、Enterobacter cowanii、Sphingobacterium multivorum、Pseudomonas putida、Pseudomonas fulva、Pseudomonas parafulva and Pseudomonas spp., respectively.

通过16S rDNA 序列系统发育分析,5 个菌株分别被鉴定为蜡状芽孢杆菌、乙酸不动杆菌、成团肠杆菌、恶臭假单胞杆菌、成团泛菌属,作为内生细菌存在于油菜体内属首次报道。

The metabolites of endophytes fungus was developed with 2D-TLC, and antibacterial active components was detected and tracked by bioautography of Bacillus subtilis model and TTC coloration. Result: The results showed that the development system screening was easy, and antiblastic spot was accurate and limpid.

以二维薄层色谱将金钱松内生真菌代谢产物样品展开,以枯草芽孢杆菌为指示菌,氯化三苯四氮唑为显色剂,采用培养基覆盖生物自显影技术对其抗细菌活性成分进行检测、追踪。

It was identified to be Bacillus cereus and proved to be able to colonize inside of tea-plant.

分离到一株能在茶树体内内生定殖并对氯氰菊酯降解效能高的菌株TR2,经鉴定为蜡状芽孢杆菌。

Bacillus cereus strain 905 isolated from wheat is an endophytic beneficial bacterium that can promote plant growth.

蜡样芽孢杆菌905(Bacillus cereus 905)是从小麦体内分离获得的一株植物有益内生细菌。

The endophytic bacteria E1R-J is isolated from the root of winter wheat,which has the better effect to take-all,powdery mildew and rust of wheat.

细菌E1R-J是一株对小麦全蚀病、小麦白粉病和小麦条锈病有拮抗作用的小麦内生芽孢杆菌,笔者对其产菌的发酵培养条件进行了初步研究。

Experimental eventuate gives birth to solution inside further research the function with enzymatic cellulose of TB2 of amylaceous gemma bacili and application laid a foundation.

实验结果为进一步探究内生解淀粉芽孢杆菌TB2纤维素酶的功能和应用打下了基础。

Effect of entophytic bacteria strain E1R-J on cabbage and radish seed germination, seedling growth was studied in plates, pot and outdoor field experiment.

通过萝卜、白菜的种子萌发试验、幼苗盆栽试验以及小区试验,对小麦内生枯草芽孢杆菌E1R-J菌株的促生作用进行了研究。

Result3 strains of endophytic fungi had growth peak when the pH value was 6.5-7.5,and could grow within pH of 4.0-8.5. Their acid-alkali adaptability was strong,in which the P-3 adaptability was the strongest. P-1and P-2 had the stronger inhibition to the Bacillus subtilis,Escherichia coli and Staphylococcus aureus,and P-3 only had the little inhibition to Escherichia coli. The 3 endophytic fungi all had inhibitory effect on Podosphaera leucotrlcha,Alternaria mali,and Gymnosporangium yamadae Miyabe,in which,P-1 had the better inhibitory ability.

结果3株内生真菌在pH值6.5~7.5出现生长最高峰,在pH值4.0~8.5都可以生长,酸碱适应性很强,其中P-3的适应性最强。P-1和P-2对枯草芽孢杆菌、大肠杆菌、金黄色葡萄球菌都有较强的抑制能力,而P-3只对大肠杆菌有很微弱的抑制能力。3株内生真菌对苹果锈病病原菌、苹果白粉病病原菌、苹果落叶病病原菌都有抑制作用,其中P-1的抑制能力较强。

The control effects were above 66%. According morphologic, physiological and biochemical characteries, strain YN-55, NN-3, YN-4 and YN-10 were identified.Some conditions for culture YN-55, NN-3, YN-4 and YN-10 were studied in shake flask (100ml in 150ml flask).

本文根据形态特征和生理生化性状,初步鉴定内生细菌YN-55为假单胞杆菌属铜绿假单胞菌,NN-3为芽孢杆菌属枯草芽孢杆菌,YN-4、YN-10为芽孢杆菌属凝结芽孢杆菌。

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