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These five strains were identified as Bacillus cereus、Acinetobacter calcoaceticu、Pantoea agglomrans、Pseudomonas putida and Pantoea ananas by 16S rDNA gene sequence phylogenetic analysis, respectively. These bacteria species is the first reported in oilseed rape rape.

本研究显示从油菜内生细菌中可筛选到拮抗作用好的菌株,并且内生细菌可产生具有抑菌活性的代谢物,可以作为对多种植物病原真菌具有抑菌活性物质的筛选资源。

Total 122 entophytic bacterial strains were categorized preliminarily to 35 groups based on colony morphology. 16S rDNAs were amplified with universal primers of bacteria, all the strains could produce a single band about 1500bp. The PCR products were digested with restriction endoenzyme HaeⅢand Msp Ⅰrespectively, and the different patterns were obtained for each enzyme, by combining all the restriction patterns for the two enzymes, the 122 strains could be grouped into 39 16S rDNA genotypes. The results indicated that endophytic bacteria from oilseed rape were genetically diversity.

分离获得的122株内生细菌,根据菌落形态可以划分为35类;利用细菌通用引物对16S核糖体DNA进行扩增,获得约1.5 kb片段,分别用内切酶HaeⅢ和MspⅠ对扩增产物进行限制性酶切,产生不同的酶切图谱;根据酶切图谱聚类分析结果,所有供试菌株被归为39类,这一结果从遗传上显示油菜内生细菌类群的多样性。

This paper tries to theoretically discuss the west area Civil-run economy , in order to have an integral and general understanding.

西部地区经济的发展关键是要建立生机勃勃的内生机制,这种内生机制的建立有赖于民营经济的发展与完善。

The results show that the nodules of Podocarpus plant are created by the endophytic bacteria.

本项目对5种罗汉松属植物的根瘤结构进行了系统观察,检测其内生细菌在宿主和豆科植物上的侵染致瘤能力,并对根瘤中的内生细菌进行了鉴定。

Result3 strains of endophytic fungi had growth peak when the pH value was 6.5-7.5,and could grow within pH of 4.0-8.5. Their acid-alkali adaptability was strong,in which the P-3 adaptability was the strongest. P-1and P-2 had the stronger inhibition to the Bacillus subtilis,Escherichia coli and Staphylococcus aureus,and P-3 only had the little inhibition to Escherichia coli. The 3 endophytic fungi all had inhibitory effect on Podosphaera leucotrlcha,Alternaria mali,and Gymnosporangium yamadae Miyabe,in which,P-1 had the better inhibitory ability.

结果3株内生真菌在pH值6.5~7.5出现生长最高峰,在pH值4.0~8.5都可以生长,酸碱适应性很强,其中P-3的适应性最强。P-1和P-2对枯草芽孢杆菌、大肠杆菌、金黄色葡萄球菌都有较强的抑制能力,而P-3只对大肠杆菌有很微弱的抑制能力。3株内生真菌对苹果锈病病原菌、苹果白粉病病原菌、苹果落叶病病原菌都有抑制作用,其中P-1的抑制能力较强。

Endophtic bacteria strains were isolated from healthy tobaccos from heavy disease plots in Yongzhou, Ningxiang and Liuyang. By Laboratory dual culture test, seventeen actinomycetes to Phytophthora parasitica var. nicotianae were screened.

同时在湖南永州、浏阳和宁乡等地发病重的田块采集健株共分离到内生细菌120株,经室内平皿对峙培养法测定,其中8株内生细菌菌株在室内有较强的拮抗作用。

Prior research on capital structure by Titman (1988) utilizes structural equation models to find out the determinants of capital structure. In this paper, we use the same methodology and add another endogenous variable – stock return to construct two equations. One regards capital structure and the other one regards stock returns.

Titman (1988)曾利用求解结构方程模式的统计软体–LISREL找寻影响公司资本结构的隐藏因子,本文乃以此篇论文为基础并加以延伸,除了纳入原先的内生变数资本结构外,再加入另一个内生变数–股票报酬率来建立一个双向影响的结构联立方程式,以期研究资本结构与股票报酬率两者是如何交互影响,并是如何由许多因子所共同决定。

At same time,studies have much progress in classification and identification of endophytes,especially after the application of molecular biology techniques in it.

与此同时,植物内生菌分类鉴定的研究也有一定的进展,特别是在分子生物学技术应用到其中后。基于这点,就最近10年来有关植物内生菌分类鉴定的文献所使用的技术进行了分析和讨论。

The result of the antibacterial and antifungal assay shows that thirty five strains of the endofungi (58.3% of total tested strains) have antibacterial activities, the active strains mostly belong to sterile groups (51.4% of active strains), Penicillium (8.6%), Aspergillus (8.6%), Fusarium(8.6%); forty nine strains (81.7% of total tested strains) have antifungal activites, most of them belong to sterile groups (44.9% of active strains), and Gloeosporium (17.1%).

结果 共有35株内生真菌具有抗细菌活性,占待侧菌株总数的58.3%,其中以不产孢类、青霉属、曲霉属、镰孢属为主,分别占活性菌株总数的 51.4%、8.6%、8.6%、8.6%;共有49株内生真菌具有抗真菌活性,占待侧菌株总数的81.7%,其中以不产孢类和盘长孢属为主,分别占活性菌株总数的44.9%、17.1%。

The optimized conditions for isolation and culture of endophytic bacteria isolated from grasses in alpine area in this study. The different tissues of grasses were immersed in 0.1% of SDS for 15 min, 3% of NaClO for 3 min, 0.1% of mercuric chloride for 10 min, 75% of alcohol for 1 to 2 min. The endophytic bacteria were isolated after aseptic grinding and diluted to 10^(-3) and using TSA culture medium for 5 to 7 days under 28℃.

通过对高寒牧草内生细菌的分离培养方法研究,得出了优化的分离培养条件,即牧草不同组织器官用0.1%SDS浸泡15min、3%NaClO浸泡3min0.1%升汞浸泡10min、75%酒精1~2min处理后(各步间均用无菌水冲洗3~4次),研磨并稀释至10^(-3),涂布于TSA培养基上,置28℃恒温条件下培养5-7d,可从高寒牧草组织中分离获得数量、种类较多的内生细菌。

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The dynamic variation in content of cndogenous abscisic acid,indoleacetic acid,gibberellinsand isopentenyladenodines of normal juvenilefruit and dropped fruit was measured with enzyme linked immunosorbent assayin the course of physiological fruit drop of 'Shuizhang Longan.

应用酶联免疫法测定了水涨龙眼生理落果期间正常幼果和脱落幼果中内源激素ABA、IAA、GAs及iPAs含量的变化。

Zillions of code examples on the web.

网上数量巨大的代码例程。

MARY, QUEEN OF SCOTS 1542-1587 Oil on panel, 79.190.2 (311/8 × 351/2) By an unknown artist after miniature by Nicholas Hilliard, inscribed, c.

1542年,作为苏格兰国王詹姆斯五世和法国吉斯家族的玛丽之女,玛丽尚在襁褓之中便继承了父亲的王位。