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In this research, swainsonine contents of three Oxytropis species were determined by the high performance liquid chromatography coupled to mass spectrometry. The population genetic diversity was also studied and the swainsonine related molecular markers were screened by ISSR. In vitro fungi cultures from the individual plant of O. glabra were performed and microbiological morphology of the colony and the conidiophore were investigated. The 5.8S rDNA/ITS sequences of fungi amplified from total DNA in both plants and fungal endophytes were studied. These sequences were analysed and compared, and they were also compared to the 5.8S rDNA/ITS sequence of fungal enduphyte Embellisia sp. L12 from the O.lambertii, O.sericea and A.mollisimus distributed in USA.

本研究采用高效液相色谱-质谱联用法测定了三种棘豆植物的苦马豆素含量;通过ISSR分子标记技术对三种棘豆种群遗传多样性及其苦马豆素相关标记进行了研究;将小花棘豆植物单株进行体外真菌培养,研究了菌落和分生孢子的微生物形态学特性,从植物总DNA和相同植株培养的真菌总DNA扩增真菌的5.8SrDNA/ITS区,进行了DNA序列分析与排序;联系单株小花棘豆的苦马豆素含量测定和内生真菌ITS序列分析结果,与从美国分布的二种棘豆(O.lambertii、O.sericea)和一种黄芪(A.mollisimus)中分离的内生真菌埃里砖格孢属Embellisia sp.L12株得到的结果进行了比较。

The endophyte is surrounded by a membrane of apparent host plant origin .

内生植物是明显地被起源于寄生植物的膜包围的。

The results show that the nodules of Podocarpus plant are created by the endophytic bacteria.

本项目对5种罗汉松属植物的根瘤结构进行了系统观察,检测其内生细菌在宿主和豆科植物上的侵染致瘤能力,并对根瘤中的内生细菌进行了鉴定。

Arbuscular Mycorrhiza is one of the most broad mycorrhizal, it can enhance plants' ability of absorbing.

丛枝菌根是分布最广泛的一类内生菌根,它可以促进植物对水分的吸收和利用,提高植物的抗旱能力。

The primary studies on insecticidical and fungicidical components of endophytic fungus in Celastrus angulatus have been carried out from September, 2000 to June, 2002.The results are as follows: Taking PDA and Czapek as separating culture medium, 39 strains of endophytic fungus were separated from the bark, phloem and xylem of the root and twig of Celastrus angulatus.

作者于2000年9月至2002年6月对杀虫植物苦皮藤中内生真菌的杀虫杀菌活性物质进行了初步的研究,取得了以下结果:采用常规的内生真菌分离方法,以马铃薯葡萄糖培养基、Czapek培养基为分离用培养基,从苦皮藤根和侧枝的表皮、韧皮部和木质部中分离得到39株内生真菌。

A number of anti-plant pathogenic fungi natural compounds with diverse structures have been isolated from endophytic fungi.

植物内生真菌的代谢产物中存在一系列具有多样性结构的抗植物病原真菌的活性化合物。

The botanical properties of the host plant and the endophyte stromata without sexual structures indicate that this symbiotum should be a brand new host-endophyte association.

宿主植物的特殊性和菌株的子座形成能力显示了这是一个禾本科植物-内生真菌的新组合。

With the development of research in the field of endophytes, the role of secondary metabolites of endophytes in drug discovery has been greatly enhanced in the past few years.

随着对植物内生菌的研究日益深入,内生菌次生代谢产物的研究趋向活跃。研究表明,内生菌具有丰富的次生代谢产物多样性。

Diversity of endophytic fungi in leaves of Ginkgo biloba was studied in order to understand diversity state and succession change of endophytic fungal communities in this endemic plant. Healthy leaf samples were collected from five main planting locations of G. biloba in Jiangsu and Shandong provinces separately in spring, summer and autumn.

为了解我国特有植物银杏的叶部组织中内生真菌菌群的多样性和演替变化,分别于春、夏、秋季从江苏和山东两省的五个主要银杏栽培地采集健康叶片进行了内生真菌的研究。

Diversity of endophytic fungi in leaves of Ginkgo biloba was studied in order to understand diversity state and succession change of endophytic fungal communities in this endemic plant. Healthy leaf samples were collected from five main planting locations of G. biloba in Jiangsu and Shandong provinces separately in spring, summer and autumn.

摘 要:为了解我国特有植物银杏的叶部组织中内生真菌菌群的多样性和演替变化,分别于春、夏、秋季从江苏和山东两省的五个主要银杏栽培地采集健康叶片进行了内生真菌的研究。

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