内生植物

These five strains were identified as Bacillus cereus、Acinetobacter calcoaceticu、Pantoea agglomrans、Pseudomonas putida and Pantoea ananas by 16S rDNA gene sequence phylogenetic analysis, respectively. These bacteria species is the first reported in oilseed rape rape.

本研究显示从油菜内生细菌中可筛选到拮抗作用好的菌株，并且内生细菌可产生具有抑菌活性的代谢物，可以作为对多种植物病原真菌具有抑菌活性物质的筛选资源。

Three developing systems were used for chromatography of TLC. UV 254nm, 366nm and anisaldehyde as a chromogenic agent were used for detecting compounds of these strains.

本研究设计4种内生菌发酵培养基，对89株分离自傣药植物且具有高抗菌活性的内生菌及5株潜在放线菌新种进行摇瓶发酵，发酵液的提取物用3种展层系统作薄层色谱展层，用UV(254nm、366nm)和茴香醛试剂进行检测。

In this research, swainsonine contents of three Oxytropis species were determined by the high performance liquid chromatography coupled to mass spectrometry. The population genetic diversity was also studied and the swainsonine related molecular markers were screened by ISSR. In vitro fungi cultures from the individual plant of O. glabra were performed and microbiological morphology of the colony and the conidiophore were investigated. The 5.8S rDNA/ITS sequences of fungi amplified from total DNA in both plants and fungal endophytes were studied. These sequences were analysed and compared, and they were also compared to the 5.8S rDNA/ITS sequence of fungal enduphyte Embellisia sp. L12 from the O.lambertii, O.sericea and A.mollisimus distributed in USA.

本研究采用高效液相色谱-质谱联用法测定了三种棘豆植物的苦马豆素含量；通过ISSR分子标记技术对三种棘豆种群遗传多样性及其苦马豆素相关标记进行了研究；将小花棘豆植物单株进行体外真菌培养，研究了菌落和分生孢子的微生物形态学特性，从植物总DNA和相同植株培养的真菌总DNA扩增真菌的5.8SrDNA/ITS区，进行了DNA序列分析与排序；联系单株小花棘豆的苦马豆素含量测定和内生真菌ITS序列分析结果，与从美国分布的二种棘豆(O.lambertii、O.sericea)和一种黄芪(A.mollisimus)中分离的内生真菌埃里砖格孢属Embellisia sp.L12株得到的结果进行了比较。

The endophyte is surrounded by a membrane of apparent host plant origin .

内生植物是明显地被起源于寄生植物的膜包围的。

The results show that the nodules of Podocarpus plant are created by the endophytic bacteria.

本项目对5种罗汉松属植物的根瘤结构进行了系统观察，检测其内生细菌在宿主和豆科植物上的侵染致瘤能力，并对根瘤中的内生细菌进行了鉴定。

Methods The antibacterial and antifungal activities test of fungal endophytes from mangrove plants were used by paper disc method. Results Sixty strains of endophytic fungi were isolated from Aegiceras corniculatum, Excoecaria agallocha, Kandelia candel, Bruguiera gymnorrhiza, Avicennia mariana, Heritiera littoralis.

从红树林植物桐花树、海漆、秋茄、木榄、白骨壤、银叶树中分离到60株内生真菌，对其代谢产物进行抗菌检测，通过滤纸片法来检测内生真菌代谢产物抗细菌和抗真菌活性。

The primary studies on insecticidical and fungicidical components of endophytic fungus in Celastrus angulatus have been carried out from September, 2000 to June, 2002.The results are as follows: Taking PDA and Czapek as separating culture medium, 39 strains of endophytic fungus were separated from the bark, phloem and xylem of the root and twig of Celastrus angulatus.

作者于2000年9月至2002年6月对杀虫植物苦皮藤中内生真菌的杀虫杀菌活性物质进行了初步的研究，取得了以下结果：采用常规的内生真菌分离方法，以马铃薯葡萄糖培养基、Czapek培养基为分离用培养基，从苦皮藤根和侧枝的表皮、韧皮部和木质部中分离得到39株内生真菌。

The results indicated that the separation probability and the quantity of endophytic bacteria varied with seasons and organs.

研究发现内生菌具有丰富的生物多样性，对一种植物而言，分离到的内生真菌和细菌达数种至数十种，有的多达数百种。

At same time,studies have much progress in classification and identification of endophytes,especially after the application of molecular biology techniques in it.

与此同时，植物内生菌分类鉴定的研究也有一定的进展，特别是在分子生物学技术应用到其中后。基于这点，就最近10年来有关植物内生菌分类鉴定的文献所使用的技术进行了分析和讨论。

With the development of research in the field of endophytes, the role of secondary metabolites of endophytes in drug discovery has been greatly enhanced in the past few years.

随着对植物内生菌的研究日益深入，内生菌次生代谢产物的研究趋向活跃。研究表明，内生菌具有丰富的次生代谢产物多样性。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。