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内生孢子

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In this research, swainsonine contents of three Oxytropis species were determined by the high performance liquid chromatography coupled to mass spectrometry. The population genetic diversity was also studied and the swainsonine related molecular markers were screened by ISSR. In vitro fungi cultures from the individual plant of O. glabra were performed and microbiological morphology of the colony and the conidiophore were investigated. The 5.8S rDNA/ITS sequences of fungi amplified from total DNA in both plants and fungal endophytes were studied. These sequences were analysed and compared, and they were also compared to the 5.8S rDNA/ITS sequence of fungal enduphyte Embellisia sp. L12 from the O.lambertii, O.sericea and A.mollisimus distributed in USA.

本研究采用高效液相色谱-质谱联用法测定了三种棘豆植物的苦马豆素含量;通过ISSR分子标记技术对三种棘豆种群遗传多样性及其苦马豆素相关标记进行了研究;将小花棘豆植物单株进行体外真菌培养,研究了菌落和分生孢子的微生物形态学特性,从植物总DNA和相同植株培养的真菌总DNA扩增真菌的5.8SrDNA/ITS区,进行了DNA序列分析与排序;联系单株小花棘豆的苦马豆素含量测定和内生真菌ITS序列分析结果,与从美国分布的二种棘豆(O.lambertii、O.sericea)和一种黄芪(A.mollisimus)中分离的内生真菌埃里砖格孢属Embellisia sp.L12株得到的结果进行了比较。

Based on phenotypical and biochemical properties as well as its 16S rRNA gene sequence, the bacterium was identified as Paenibacillus polymyxa. In vitro antifungal spectrum assay vindicate that it was active against a broad range of phytopathogens and inhibited the growth of thirteen fungal pathogens.

采用对峙培养法筛选拮抗内生细菌,根据菌株形态、生理生化特性结合16S rRNA基因序列分析鉴定菌株;采用硫酸铵沉淀法提取抗菌粗蛋白,研究其对水稻恶苗病菌菌丝生长和孢子萌发的影响。

Based on morphological characteristics,351 strains of endophytes were isolated and classified into 7 orders,8 familes,and 29 genera.

的叶、枝和干皮,从中分离获得内生真菌351株(其中23株未产生孢子),经显微形态观察分类鉴定为7目8科29属。

Endospores can remain viable for several centuries and are resistant to heat, desiccation, and x-rays.

内生孢子可以存活几个世纪,能够耐热、忍受干燥以及抵御 X-射线损害。

Endospores are formed within the cell, one in each parent cell, and are surrounded by a thick coat containing dipicolinic acid.

内生孢子是在细菌细胞内形成的,且每一个母细胞只形成一个,形成后被含有2,6吡啶二羧酸的厚壁所包围。

Th is pulmonary infection is due to Coccidioidomycos is , as evidenced by the two large spherules filled with endospores.

粗球孢子菌病肺部感染灶,可见两个大球体充满内生孢子

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