内源

The direct interaction of copper ion with CuZnSOD was studied by fluorescence spectroscopy, and a type of 1∶1 stable complex has been generated, which was certified by the quenching experiment of intrinsic fluorescence. The process of the generated complex shown by dynamic analysis of fluorescence quenching, consisted of two steps, in which the first step was a quick association process of double molecules to generate a loose complex, and the second step was a slow process of single molecule, where the loose complex was isomerized into the compact complex.

通过荧光光谱方法研究了这种相互作用，内源荧光的猝灭实验表明Cu2＋与CuZnSOD形成1∶1型稳定配合物；荧光猝灭的动力学分析表明配合物形成过程由两个独立步骤完成：第一步是双分子快速缔合过程，形成了结合疏松的配合物，第二步是单分子慢速过程，即松散的配合物&异构化&成为结合紧密的配合物。

It was shown here that lentivirus containing FSP27siRNA can effectively knockdown FSP27 expression in 3T3-L1 cells.

结果显示，该siRNA可以高效地抑制内源小鼠FSP27的表达，并且siRNA插入基因组中，形成稳定表达。

As endogenous jasmonates exist in enantiomers in nature and at level of nanogram/per gram fresh weight, it is very challenging work to develop rapid and accurate method for qualitative and quantitative analyses of JAs. This review paper summarizes the recent advances in the analysis of jasmonates including chromatography, capillary electrophoresis, chromatography-mass spectrometry and immunoassay.

内源茉莉酸类激素通常以多种手性异构体存在，且含量超微，因此，对内源性茉莉酸类激素进行准确的定性、定量分析具有很大的难度，研究高效分离富集、高灵敏度检测以及分离与检测联用等分析方法，对加速植物激素分子作用机理研究具有重大意义。

In this paper the Guinong No.5selection of Rosa roxburghii Tratt was used as material for studying the changes of the contents of ZRs ,IAA,ABA,starch,total water soluble sugar and total nitrogen at the flower bud differentiation stage.

在对贵农5号刺梨花芽分化进行观察的基础上，对花芽分化期刺梨花芽和叶芽中内源激素的赤霉素(GA1+3)、玉米素核苷、生长素、脱落酸和淀粉、可溶性总糖、总氮含量变化进行了研究。

Rosa roxburghii Tratt ; Flower bud differentiation ; Endogenous hormones ; Carbohydrate ; Nitrogen nutrition

刺梨；花芽分化；内源激素；碳水化合物；氮素营养

Moreover, the increase of contents of IAA, ABA and JA would inhibit the elongation of stolons, whereas, promote tuber formation.

而内源IAA、ABA及JA含量的增加则会抑制匍匐茎的伸长及促进块茎的形成。

The results of its intrinsic fluorescence spectroscopy and fluorescence phase diagram showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed a partially folded intermediate of Bacillus amyloliquefaciens a-amylase during its unfolding procedure, which followed a three-state model; the result of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed some stable hydrophobic regions, which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid, in the partially folded intermediate of Bacillus amyloliquefaciens a-amylase; and the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed the distribution of Trp residues in Bacillus amyloliquefaciens a-amylase in different denaturation solution, with the maximum number (8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciens a-amylase molecule could be quenched by potassium iodide; and the results of their protein electrophoresis and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciens a-amylase formed during the whole unfolding procedure of Bacillus amyloliquefaciens a-amylase induced by guanidine hydrochloride.

内源荧光光谱和荧光相图结果表明，当变性液中盐酸胍浓度约为1.0 mol/L时，芽孢杆菌a-淀粉酶的去折叠过程中出现一个部分折叠中间体，其去折叠过程符合&三态模型&；荧光探针结果表明，在溶液中盐酸胍浓度约为1.0 mol/L时，中间态芽孢杆菌a-淀粉酶分子中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；荧光猝灭研究给出了不同程度变性的淀粉液化芽孢杆菌a-淀粉酶中的Trp的分布情况，结果表明中间态芽孢杆菌a-淀粉酶分子中能够被碘化钾猝灭的位于分子表面的色氨酸残基数目达到最大的8个；蛋白电泳和体积排阻色谱结果表明，在盐酸胍诱导的芽孢杆菌a-淀粉酶分子的整个去折叠过程中，不会以共价键或非共价键形式形成芽孢杆菌a-淀粉酶分子之间的集聚体或集聚体沉淀。

Endogenous hormone ABA and GA3 played an important role in seed dormancy and germination. ABA was the key factor for seeds germination, the reduction of ABA was the initiatory factor for embryo development, while the increased GA3 benefited physiological after-ri pening.

内源激素ABA和GA3在滇重楼种子休眠与萌发过程中起着重要作用，ABA是引起休眠的关键因素，ABA含量的降低是滇重楼种胚发育的始动因子，GA3有助于种子的生理后熟。

At present, financing has become an actual problem for the high-tech enterprises in China and has been restricting their persistent and quick development. On the one hand, many high-tech enterprises are founded by some scientific researchers or patentees and are characterized by small scale and nonstandard management, so their capital demands can't be satisfied by internal self-cumulative financing. On the other hand, these high-tech enterprises have to face high technical, market, financial and management risks at their incubation, start-up and growth stages;it's hard for them to acquire capital by external financing.

目前，融资难已成为制约我国高新技术企业持续、快速发展的现实问题：一方面，许多高新技术企业是由部分科研人员或专利发明人组建而成，企业规模较小，管理不规范，无法通过自我积累式的内源融资来满足企业资金需求；另一方面，由于高新技术企业在种子期、初创期和成长期面临着较大的技术风险、市场风险、财务风险和管理风险，很难通过外源融资获取资金。

The relationship between embryonic development and changes of endogenous hormones in litchi ovules was studied.

以兰竹荔枝胚胎发育时期的正常胚珠与败育胚珠为试验材料，研究了胚胎发育与内源激素的关系。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。