内浆

The re was no significant difference between the contents of SP and VIP of lung tissue in experiment group and those in control group.4. Ultrastructurally, after infected with IBV, lots of grume were observed on the surface of trachea tissue and the cilia of the trachea were massed.

免疫电镜观察结果显示，大多数肾小管上皮细胞胞浆内均见有SP及VIP阳性颗粒，在上皮细胞间还可见有DNES细胞；肺脏中异嗜性白细胞颗粒也常呈SP及VIP阳性反应，肺脏内也可见DNES细胞。

While 30μmol·〓 FRCRSFa and 10μmol·〓 E-4031 were dialysed intracellularly via the patch-pipette, FRCRSFa significantly decreased 〓 exchange current and there was no the further decrease after adding FRCRSFa in the perfused solution with the same concentration, whereas E-4031 dialysed had no effect on 〓 exchange current, but a obvious increase of it occurred when adding E-4031 to the perfused solusion with the same concentration.

分别将30μmol·〓的FRCRSFa和10μmol·〓的E-4031加入微电极内液中，FRCRSFa显著抑制了〓交换电流，当在细胞外灌流液中加入同样浓度的FRCRSFa时无进一步抑制；而胞浆内的E-4031对〓交换电流无显著作用，而在灌流液中加入同样浓度的E-4031后，却显著增强了〓交换电流。

The expression of elastase mRNA was found in most hepatic cytoplasm. The level of its expression varies in different hepatocytes situated in the same liver lobule.

多数肝细胞的胞浆内有弹力蛋白酶mRNA的表达，其强度因小叶内肝细胞部位的不同而有所差异。

Mitochondria was relatively little in size. Round primary lysosome with high electron-densed granules and secondary lysosome with high or low electron-densed granules were seen frequently. DCs contained many rough endoplasmic reticulum, the Golgi apparatus and ribosomes. The vacuoles with flocculent electron-densed granules were rare. Some special granules in cytoplasm were seen, whose surface like earphone were covered with a membrane. High electron-densed contents in the granules were near one side and the other side was bright. The nucleus became markedly small in volume, nephroid or hoofed in shape. The nucleus had little euchromatin and lots of heterochromatin under nuclear membrane.

子宫内膜癌组织DC超微结构特征如下：细胞形态不规则，与正常子宫内膜组织DC相比，胞膜较光滑，胞膜表面树突状胞浆突起显著减少，部分突起呈粗短状；胞质中线粒体相对少，圆形而电子密度高的初级溶酶体和不规则形且电子密度高低不一的次级溶酶体多见；高尔基体、粗面内质网、核糖体丰富；含微量絮状电子致密物的胞饮小泡显著减少；胞质中可见形态特殊的颗粒，该颗粒外周膜包裹，略呈圆形，中间部位稍弯曲，如耳机状，颗粒中由高电子致密物居于一侧，而另一侧则呈透亮状；胞核显著减小，居于胞质一侧，常呈肾形或马蹄形，核内常染色质较少，异染色质多边集于核膜下。

No mature organelle was found except for some rough endoplasmic reticulum and mitochondria.

细胞浆内有丰富的粗面内质网和线粒体，其他细胞器很少见。

Positive staining of Stro-1 was found in 7.6% of SCAP. Morphologically, PDLSCs were fusiform-shaped, with many short and long ramifications under a scanning electron microscope, and the secreted extracellular matrix were around them.

透射电镜下可见，第1～3代牙周膜干细胞及根尖牙乳头干细胞生长良好，胞浆内线粒体，高尔基体和粗面内质网丰富，随着培养时间的延长，分泌的细胞外基质增多。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Results: we estalished ideal copd models in stort time. there existed the expression of nos in both the normal lung tissue and the lung tissue of copd,mainly orienrared in the cytoplasm of cell.the immunohistochemical result indicated that inos increased significantly ,in comparison with the control group, in bronchiolar mycoderma epithelial and enos remakably decreased in pulmonary blood vessel.

结果：联合应用烟熏和ppe在较短的时间内建立了较理想的copd模型；一氧化氮合酶在正常肺组织与copd的肺组织均有表达，主要定位在细胞浆内；免疫组化结果表明模型组inos在支气管黏膜上皮呈强阳性表达，肺血管enos表达明显减弱，与健康对照组比较有显著性（p.01）。

According to analysis of flow field and velocity distribution in the mix tank,the results indicate that:using draft-tube in the mix tank,the axial flowing of liquid can be increased,the circumgyration around the impeller will be improved,and the strong mixed effect can be gained.

通过对有、无导流筒的搅拌槽内流场和速度的对比分析表明：导流筒强化了搅拌槽内上下液体的整体轴向流动，在近浆区域改善了液体的跟转，有强烈的混合效果，并且，在较为理想的流场分布下，有导流筒的搅拌槽功率消耗比无导流筒搅拌槽节省12%，其结果将为搅拌槽的整体结构优化提供有效途径。

Although ERβ was also seen in these cells,there was irregularity of its distribution.Weak to moderate cytoplastic staining of ERβ in epithelial cells of lobules and ducts were fonud.

ERβ在小叶上皮细胞和导管上皮细胞的细胞核内检测到，但是ERβ在上皮细胞中的分布并无规律，而且在细胞浆内也可以检测到弱到中等强度的染色。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。