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The typical apoptotic morphological features appeared in MUTZ-1 cells treated with 4mmol/L VPA for 72hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dose-dependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dose-dependent manner, the cells were arrested at G0/G1 phase.
结果显示:VPA对MUTZ-1细胞的生长抑制作用呈现时间和剂量依赖性;经4mmol/LVPA处理MUTZ-1细胞72小时后,细胞呈现典型的凋亡形态特征,光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体;透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加,胞装内大小不规则的染色质团块;流式细胞术结果表明,细胞凋亡率随着VPA浓度的增加而逐步增高,G0/G1期细胞比例随着VPA浓度的增加而逐渐增多,S期细胞比例逐渐减低,细胞被阻滞在G0/G1期。
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Genetic diversity analysis within and between the green peafowl and the blue peafowl populations demonstrated that the expected heterozygosity of two peafowl populations were 0.2482 and 0.2744, respectively. The inbreeding index, Reynolds' genetic distance and gene flow between the two populations were 0.078, 0.0603 and 3.896 respectively. These results indicate that the heterozygosity and the genetic diversity of these two peafowl populations were very low, and suggest a tendency towards intermixing.
蓝孔雀和绿孔雀群体间和群体内的遗传分析结果表明,绿孔雀和蓝孔雀两个群体的期望杂合度分别为0.7422和0.6943,群体间的遗传分化系数为0.078, Reynolds'遗传距离和基因流分别为0.0603和3.896,结果显示这两个孔雀群体的杂合度和遗传多样性水平都很低,且有相互混杂的趋势。
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Based on the analysis of Sea Surface Height Anomaly from satellite observation, it is proved that the oscillation with period of 70~210 days is dominating in two branches of the eastward countercurrent in Subtropical North Pacific: Subtropical Countercurrent and Hawaiian Lee Countercurrent.
根据对卫星观测的海平面高度资料的分析,进一步证实了在北太平洋副热带两支向东的流(副热带逆流和夏威夷背风逆流)所在的区域内,海平面高度的70~210天周期振荡是主要的低频变化。
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Comparison between the velocity distributions at the different flow rate conditions showed that increasing the flow rate was help to improving the velocity distribution. The periodic mean field on r-6 plane at different axis positions showed that the flow within the vaned diffuser, especially within the inlet region, was periodic unsteady when impeller located at the different circumferential position, just as the flow within the vaneless diffuser.
有叶扩压器内平均流场的分析表明,在定常流动的假设下,不同流量工况下有叶扩压器的内部流动也呈现为沿轴线向后盘方向推移流动状况逐渐恶劣的分布规律,表明二元叶片构成的平行直壁有叶扩压器对轴向不均匀流动的掺混作用仍然不大。
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Results: The direct signs (such as mural nodule of cystic lesion, solid neoplastic component of solid type、 mixed type tumors enhanced markedly; vascular void signal show with in the tumors) and indirect sign (solid neoplastic component of the tumors close to or adhesion to a pia mater) have some specific manifestations on MR imaging...
结果:血管母细胞瘤的直接征象(如囊肿型的瘤结节,实质性、混合性肿瘤的实质部分明显强化;瘤内出现血管流空信号)及间接征象(肿瘤实质部分邻近软脑膜或与软脑膜有粘连)具有一定的特异性。结论:MR对诊断血管母细胞瘤具有较高的价值。
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After rational predigestion of the heat exchanger of the thermoelectric generator''s physical model in this paper,by using the Fluent software,adopting the SIMPLE arithmetic and k-εmodel,the 3D inner flow of the heat exchanger of the thermoelectric system was simulated numerically.
本文在对温差发电系统的冷端换热器的物理模型进行了合理的简化处理后,应用Fluent通用软件,采用Simple算法和标准k-ε湍流模型,对温差发电系统的冷却端换热器内的流场进行了三维数值分析。
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The results show that dynamic softening is easy to occur in these ingots and flow stress becomes steady finally; the material constants of hot deformation are procedural parameters that stress-level coefficient α, strain rate sensibility exponent m and deformation activation energy Q are all increased with strain increasing, while stress exponent n is decreased gradually; the values α and m of the alloy homogenized are the biggest and the value n is the smallest, dynamic softening effect of the alloy is the most obvious, which can deform evenly; influence of strain on the value Q of the alloy homogenized is a little and the average value of Q is only 176.5kJ/mol, so the alloy is easy to deform; the alloy matrix shows the regular recrystallization grains when the strain is 50%; dislocation cell structure re-formed in the grains with increase of strain.
结果表明:经不同处理的Al-1Mn-1Mg合金均易发生动态软化并最终呈现稳态流变特征;热变形材料常数是过程量,随应变量的增加,应力水平参数α、应变速率敏感性指数m和热变形激活能 Q 随之增大,而应力指数 n 则逐渐减小;均匀化退火后,铝合金的α和m值最大而n值最小,动态软化效果最明显且变形均匀,在该状态下,铝合金的 Q 值受变形量影响小,平均仅为176.5kJ/mol,易进行热变形;当应变量为0.7时,基体呈现规则的再结晶晶粒组织,随着应变量的增加,晶内重新形成了位错胞结构。
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To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.
1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry,FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时,对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后,Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR,RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时,Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope,LSM)观察北豆根总碱(0、40μg/ml)作用24小时后,Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。
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The mean velocity distributions in turbulent boundary layers of smooth surface and two different riblet surfaces are measured with IFA300 constant-temperature anemometer in low-speed wind tunnel to validate the full development of turbulent boundary laye. Friction velocity and wall friction stress of turbulent boundary layer are obtained with the least-squares fitted curve of the sublayer velocity distribution based on Spalding formula, and virtual origin position of riblet surface can be acquired accurately.
在低速风洞中来流速度一定的情况下使用IFA300恒温热线风速仪测量了光滑表面和两种不同尺寸的脊状表面湍流边界层平均速度分布剖面,并验证了试验段湍流发展的充分性;通过应用Spalding壁面公式使用最小二乘法精准拟合了实验测量的边界层内层速度分布曲线,得到了湍流边界层壁面摩擦速度并进一步求得湍流壁面摩擦应力,较准确地计算出脊状表面的虚拟原点位置,并通过与对数律公式拟合结果比较分析,证实了该方法更加准确有效。
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Starting from the differential equations which describe the gas-solid flow characters in the raceway of blast furnace,and using the similarity transformation technique to derive the similarity criterion,a cold model was set up to simulate the gas and coke movement in the raceway.
从描述回旋区内气固流运动特性的微分方程出发,应用相似转换法导出相似准则,建立模拟回旋区内煤气及焦炭运动的冷态模型。冷态试验采用了大米、小米、绿豆等作为模型的填充料层;应用HG-100K高速摄像机记录了模型内回旋区的大小与颗粒运动轨迹;并详细分析了影响回旋区大小的主要因素
- 推荐网络例句
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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.
随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。
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But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.
不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。
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Closes the eye, the deep breathing, all no longer are the dreams as if......
关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。